FastQCFastQC Report
Thu 9 Feb 2017
SRR2727218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115047
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT16470.14770677827930123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12520.11228226254139961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG400.001931151423.1255
TATCCCG400.001931151423.1255
TAACGAA1750.019.0285713
GGGTAAG8600.018.51
ATAACGA1800.018.512
GGTATCA8150.018.159511
AACGAAC1950.018.02564214
AAGACGG3400.017.4117645
ATTATAC852.722998E-517.4117643
GTAAGAG9000.017.2666663
CGAACGA1950.017.07692316
ACGAACG1950.017.07692315
CAAGACG3600.016.9583344
GGTAAGA9400.016.5319162
TAGTACA904.4457658E-516.4444454
CGATAAC2050.016.24390410
TAGGGTG803.3824524E-416.18755
TTAACAC803.3824524E-416.18753
CCGATAA2100.015.8571449
CGAGACT2250.015.62222220
Produced by FastQC (version 0.11.5)