##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727218.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1115047
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.411671436271295	31.0	30.0	31.0	27.0	34.0
2	30.661248359934604	31.0	30.0	33.0	27.0	34.0
3	30.742301445589288	31.0	30.0	34.0	27.0	34.0
4	34.58999486120316	35.0	35.0	37.0	32.0	37.0
5	34.28123836932434	35.0	35.0	37.0	30.0	37.0
6	34.41341037642359	35.0	35.0	37.0	30.0	37.0
7	34.399697053128705	35.0	35.0	37.0	30.0	37.0
8	34.40346640096785	35.0	35.0	37.0	30.0	37.0
9	35.90888276458302	37.0	35.0	39.0	30.0	39.0
10	35.5552635897859	37.0	35.0	39.0	30.0	39.0
11	35.77149124655732	37.0	35.0	39.0	30.0	39.0
12	35.55256504882754	37.0	35.0	39.0	30.0	39.0
13	35.6795040926526	37.0	35.0	39.0	30.0	39.0
14	36.54116821981495	38.0	35.0	40.0	30.0	41.0
15	36.558241042754254	38.0	35.0	40.0	30.0	41.0
16	36.47505889886256	38.0	35.0	40.0	30.0	41.0
17	36.39771148660101	38.0	35.0	40.0	30.0	41.0
18	36.28786768629484	38.0	35.0	40.0	30.0	41.0
19	36.26620850959646	38.0	34.0	40.0	30.0	41.0
20	36.13110120021847	38.0	34.0	40.0	29.0	41.0
21	35.99629881072278	38.0	34.0	40.0	29.0	41.0
22	35.89771641912852	38.0	34.0	40.0	28.0	41.0
23	35.75848103263809	38.0	34.0	40.0	27.0	41.0
24	35.578919991713356	38.0	34.0	40.0	27.0	41.0
25	35.44285128788293	38.0	34.0	40.0	27.0	41.0
26	35.130143393058766	38.0	33.0	40.0	27.0	41.0
27	34.93922318969514	38.0	33.0	40.0	26.0	41.0
28	34.68972070235604	37.0	33.0	40.0	25.0	41.0
29	34.642661699461996	37.0	33.0	40.0	25.0	41.0
30	34.57072482146493	37.0	33.0	40.0	25.0	41.0
31	34.75735821001267	37.0	33.0	40.0	25.0	41.0
32	34.7541359243153	37.0	33.0	40.0	25.0	41.0
33	34.769302997990216	38.0	33.0	40.0	25.0	41.0
34	34.85774142255887	38.0	33.0	40.0	25.0	41.0
35	34.604555682406215	37.0	33.0	40.0	25.0	41.0
36	34.56334037937415	37.0	33.0	40.0	24.0	41.0
37	34.482976950747364	37.0	33.0	40.0	24.0	41.0
38	34.39446498667769	37.0	33.0	40.0	24.0	41.0
39	34.32252272774152	37.0	32.0	40.0	24.0	41.0
40	34.1103451244656	37.0	32.0	40.0	24.0	41.0
41	34.043937161393195	37.0	32.0	40.0	23.0	41.0
42	33.81790274311307	37.0	31.0	40.0	23.0	41.0
43	33.49405630435309	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	6.0
14	6.0
15	24.0
16	45.0
17	96.0
18	224.0
19	525.0
20	1004.0
21	1955.0
22	3301.0
23	5346.0
24	8529.0
25	12198.0
26	16902.0
27	22873.0
28	29637.0
29	37238.0
30	45668.0
31	54736.0
32	64439.0
33	76017.0
34	89809.0
35	106148.0
36	128826.0
37	157923.0
38	180455.0
39	71111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.68195690405875	23.649137659668156	14.25374894511173	23.41515649116136
2	24.368120805670074	23.43614215364913	29.266927761789415	22.928809278891382
3	25.012488262826587	23.5558680486114	28.209931958025088	23.22171173053692
4	20.669352951041525	18.031885651456843	31.048556697610056	30.250204699891576
5	15.657994685425816	37.79185989469502	32.59916398142859	13.950981438450578
6	35.80432035600293	35.967721539988894	14.203168117577105	14.024789986431067
7	29.479295491580178	31.257606181622837	20.35429896677001	18.908799360026975
8	27.790936166816287	33.92798689203235	19.011844343781025	19.269232597370333
9	27.732014883677547	13.004474250861175	18.27501441643267	40.9884964490286
10	16.812833898481408	26.72873878858918	32.9013933941798	23.55703391874961
11	38.51147081692521	20.970057764381234	20.00068158561926	20.5177898330743
12	21.610568881849822	24.889713169041304	28.52991846980441	24.96979947930446
13	31.320294122131177	19.181702654686305	23.59676318576706	25.901240037415462
14	23.259737033506212	20.231972284576344	23.567526749993498	32.94076393192395
15	26.562916182008472	27.710849856553132	20.495997029721615	25.23023693171678
16	26.145355307892853	25.830121958984687	23.061270063055638	24.963252670066822
17	24.97805025259025	25.644748607009394	23.82706737922258	25.55013376117778
18	24.98343119168968	24.75276826896086	25.10351581592525	25.160284723424216
19	25.692997694267593	25.197861614801887	24.86881718887186	24.240323502058658
20	25.322789084226944	24.10777303557608	24.625957470851002	25.943480409345977
21	26.224634477291094	24.812407010646186	24.56578063525573	24.397177876806985
22	26.420500660510278	24.508563316165148	23.91881239086783	25.152123632456746
23	25.40843569822617	24.510626009486597	24.633311420953557	25.44762687133367
24	25.261894790085083	24.828729192581118	24.493227639731778	25.41614837760202
25	25.94276295079938	24.19862122403809	24.215929911474586	25.642685913687945
26	25.449958611610096	25.094457901774543	24.322024094051642	25.13355939256372
27	26.592780394010298	24.715639789174805	23.703574826890705	24.988004989924193
28	25.460810172127275	25.345389028444544	24.17243398708754	25.021366812340645
29	25.49659341713847	25.29301455454344	24.605868631546475	24.604523396771615
30	26.092980833991753	25.019393801337518	24.040600979151552	24.847024385519177
31	25.98751442764296	25.17741404622406	24.23915763192045	24.595913894212533
32	25.74707613221685	24.69868983101161	23.957017058473767	25.597216978297777
33	25.081812694890885	24.705685051840863	24.34767323709225	25.864829016176
34	25.999173129025056	24.891865544681078	24.697972372465017	24.410988953828852
35	26.014508805458426	24.654745495032945	24.73357625283957	24.597169446669064
36	25.080377777797707	25.171495013214688	24.625688423896033	25.12243878509157
37	25.43381579431181	25.106834061703225	24.90872582052595	24.55062432345901
38	25.519641772947686	24.12122538332465	25.030693773446323	25.328439070281345
39	25.28691615689742	24.614478134105557	25.098672970735763	24.999932738261258
40	25.590849533696787	24.117727772910023	25.58860747573869	24.702815217654503
41	24.478788786481644	24.44192935365056	25.837117179813944	25.242164680053847
42	24.997959727258134	24.48524591340096	25.550940902042697	24.965853457298213
43	24.59304406002617	23.721690655192113	25.86366314603779	25.821602138743927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	71.0
2	71.0
3	75.5
4	80.0
5	80.0
6	170.5
7	261.0
8	269.5
9	278.0
10	397.0
11	516.0
12	516.0
13	998.0
14	1480.0
15	2182.0
16	2884.0
17	2534.0
18	2184.0
19	2184.0
20	2832.0
21	3480.0
22	3652.0
23	3824.0
24	4746.0
25	5668.0
26	5668.0
27	7266.5
28	8865.0
29	10785.0
30	12705.0
31	15559.5
32	18414.0
33	18414.0
34	22370.0
35	26326.0
36	32040.0
37	37754.0
38	43663.5
39	49573.0
40	49573.0
41	55860.5
42	62148.0
43	68311.0
44	74474.0
45	81912.5
46	89351.0
47	89351.0
48	95969.0
49	102587.0
50	104272.5
51	105958.0
52	106036.0
53	106114.0
54	106114.0
55	100816.5
56	95519.0
57	90622.0
58	85725.0
59	78465.5
60	71206.0
61	71206.0
62	60327.0
63	49448.0
64	42971.0
65	36494.0
66	30239.0
67	23984.0
68	23984.0
69	19700.5
70	15417.0
71	12708.5
72	10000.0
73	7561.0
74	5122.0
75	5122.0
76	4017.0
77	2912.0
78	2418.0
79	1924.0
80	1485.0
81	1046.0
82	1046.0
83	784.0
84	522.0
85	468.5
86	415.0
87	287.5
88	160.0
89	160.0
90	103.5
91	47.0
92	33.5
93	20.0
94	16.5
95	13.0
96	13.0
97	7.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1115047.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.12659158226309
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.91543972515885	54.45337722283541
2	8.146008816077563	10.447515607482408
3	2.725953479593696	5.2441831637446175
4	1.3179112991429998	3.380526384871716
5	0.7726702477295679	2.477435470196003
6	0.4650269244914729	1.789235499697235
7	0.32299728306131076	1.449890039713731
8	0.22864361111709794	1.1729708374399945
9	0.1769021613940757	1.0209719388363734
>10	0.8233147845575266	9.210258816748901
>50	0.056879299547910434	2.509639497371256
>100	0.04572017549222277	5.534393699995681
>500	0.0018288057924739957	0.7641824775266949
>1k	7.033868432592291E-4	0.5454193435398887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1647	0.14770677827930123	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1252	0.11228226254139961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.793646366476032E-4
2	0.0	0.0	0.0	0.0	1.793646366476032E-4
3	0.0	0.0	0.0	0.0	1.793646366476032E-4
4	0.0	0.0	0.0	0.0	1.793646366476032E-4
5	0.0	0.0	0.0	0.0	1.793646366476032E-4
6	0.0	0.0	0.0	0.0	2.690469549714048E-4
7	0.0	0.0	0.0	0.0	4.48411591619008E-4
8	0.0	0.0	0.0	0.0	4.48411591619008E-4
9	0.0	0.0	0.0	0.0	7.174585465904128E-4
10	0.0	0.0	0.0	0.0	7.174585465904128E-4
11	0.0	0.0	0.0	0.0	7.174585465904128E-4
12	0.0	0.0	0.0	0.0	7.174585465904128E-4
13	0.0	0.0	0.0	8.96823183238016E-5	7.174585465904128E-4
14	0.0	0.0	0.0	8.96823183238016E-5	8.071408649142144E-4
15	0.0	0.0	0.0	8.96823183238016E-5	8.071408649142144E-4
16	0.0	0.0	0.0	8.96823183238016E-5	8.96823183238016E-4
17	0.0	0.0	0.0	1.793646366476032E-4	8.96823183238016E-4
18	0.0	0.0	0.0	1.793646366476032E-4	8.96823183238016E-4
19	0.0	0.0	0.0	2.690469549714048E-4	8.96823183238016E-4
20	0.0	0.0	0.0	4.48411591619008E-4	8.96823183238016E-4
21	0.0	0.0	0.0	7.174585465904128E-4	8.96823183238016E-4
22	0.0	0.0	0.0	9.865055015618176E-4	8.96823183238016E-4
23	0.0	0.0	0.0	0.0012555524565332223	8.96823183238016E-4
24	0.0	0.0	0.0	0.0015245994115046272	8.96823183238016E-4
25	0.0	0.0	0.0	0.001793646366476032	8.96823183238016E-4
26	0.0	0.0	0.0	0.0027801518680378497	8.96823183238016E-4
27	0.0	0.0	0.0	0.007443632420875533	8.96823183238016E-4
28	0.0	0.0	0.0	0.01730868743649371	8.96823183238016E-4
29	0.0	0.0	0.0	0.030491988230092543	8.96823183238016E-4
30	0.0	0.0	0.0	0.053450661720985754	8.96823183238016E-4
31	0.0	0.0	0.0	0.1337163366207882	9.865055015618176E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATCG	40	0.0019311514	23.125	5
TATCCCG	40	0.0019311514	23.125	5
TAACGAA	175	0.0	19.02857	13
GGGTAAG	860	0.0	18.5	1
ATAACGA	180	0.0	18.5	12
GGTATCA	815	0.0	18.15951	1
AACGAAC	195	0.0	18.025642	14
AAGACGG	340	0.0	17.411764	5
ATTATAC	85	2.722998E-5	17.411764	3
GTAAGAG	900	0.0	17.266666	3
CGAACGA	195	0.0	17.076923	16
ACGAACG	195	0.0	17.076923	15
CAAGACG	360	0.0	16.958334	4
GGTAAGA	940	0.0	16.531916	2
TAGTACA	90	4.4457658E-5	16.444445	4
CGATAAC	205	0.0	16.243904	10
TAGGGTG	80	3.3824524E-4	16.1875	5
TTAACAC	80	3.3824524E-4	16.1875	3
CCGATAA	210	0.0	15.857144	9
CGAGACT	225	0.0	15.622222	20
>>END_MODULE