FastQCFastQC Report
Thu 9 Feb 2017
SRR2727217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559884
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100430.28211593411470715No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT64050.17992159295078153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62870.17660687820164928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54090.15194315320386845No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG38750.10885186146514887No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG37640.10573378233672782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG14900.018.2516774
AAGACGG14400.018.2430555
CTTATAC10300.015.80582637
ACGGACC15550.015.7041818
GACGGAC16150.015.2352947
TAGTACT2450.015.1020414
CGGACCA16100.015.0527959
CGAACGA8400.014.976191516
CGCAAGA16950.014.7345142
ACGAACG8400.014.53571515
TCTTATA16050.014.52336337
AGACGGA17650.014.1501436
AGAGCGA17800.014.030915
GCGCAAG17300.014.008671
TAATACC2700.013.7037044
GTAAACG8550.013.631577527
CAGAGCG18650.013.49061814
GCAAGAC20850.013.4868113
AACGAAC9100.013.41758314
ATACCGT12300.013.3861796
Produced by FastQC (version 0.11.5)