##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727217.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3559884
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.932798372081788	31.0	31.0	33.0	28.0	34.0
2	31.179761756281945	31.0	31.0	34.0	28.0	34.0
3	31.320732641850128	31.0	31.0	34.0	28.0	34.0
4	35.16085805043085	35.0	35.0	37.0	33.0	37.0
5	34.942108225998375	35.0	35.0	37.0	32.0	37.0
6	34.952731324953284	36.0	35.0	37.0	32.0	37.0
7	34.87223881452317	35.0	35.0	37.0	32.0	37.0
8	34.86438715418817	36.0	35.0	37.0	32.0	37.0
9	36.409734699220536	38.0	35.0	39.0	32.0	39.0
10	36.1263709716384	38.0	35.0	39.0	31.0	39.0
11	36.281911152161136	38.0	35.0	39.0	32.0	39.0
12	36.115248699114915	38.0	35.0	39.0	31.0	39.0
13	36.19363945566766	38.0	35.0	39.0	32.0	39.0
14	37.167432421955326	39.0	36.0	40.0	31.0	41.0
15	37.16896955069323	39.0	36.0	40.0	31.0	41.0
16	37.10908220604941	39.0	36.0	40.0	31.0	41.0
17	37.049293460123984	39.0	36.0	40.0	31.0	41.0
18	36.96573483855092	39.0	36.0	40.0	31.0	41.0
19	36.96238079667764	39.0	36.0	40.0	31.0	41.0
20	36.85295279284381	39.0	36.0	40.0	31.0	41.0
21	36.72912656704544	39.0	35.0	40.0	30.0	41.0
22	36.64318556447345	38.0	35.0	40.0	30.0	41.0
23	36.52062117754399	38.0	35.0	40.0	30.0	41.0
24	36.365865573147886	38.0	35.0	40.0	30.0	41.0
25	36.22642647906505	38.0	34.0	40.0	29.0	41.0
26	35.97957770534096	38.0	34.0	40.0	28.0	41.0
27	35.80023843473551	38.0	34.0	40.0	27.0	41.0
28	35.55430514027985	38.0	34.0	40.0	27.0	41.0
29	35.50750389619437	38.0	34.0	40.0	27.0	41.0
30	35.43150900422598	38.0	34.0	40.0	27.0	41.0
31	35.611635379130334	38.0	34.0	40.0	27.0	41.0
32	35.61175251777867	38.0	34.0	40.0	27.0	41.0
33	35.606947024116515	38.0	34.0	40.0	27.0	41.0
34	35.68750863792191	38.0	34.0	40.0	27.0	41.0
35	35.46235579586301	38.0	34.0	40.0	26.0	41.0
36	35.42040527163245	38.0	34.0	40.0	26.0	41.0
37	35.34243924802044	38.0	34.0	40.0	26.0	41.0
38	35.25682466057883	38.0	33.0	40.0	26.0	41.0
39	35.18620662920477	38.0	33.0	40.0	26.0	41.0
40	34.992880947806164	38.0	33.0	40.0	25.0	41.0
41	34.93501164644691	38.0	33.0	40.0	25.0	41.0
42	34.719620639324205	38.0	33.0	40.0	24.0	41.0
43	34.38577717700914	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	4.0
14	13.0
15	51.0
16	118.0
17	259.0
18	485.0
19	890.0
20	1915.0
21	3797.0
22	6896.0
23	11448.0
24	18521.0
25	27816.0
26	40254.0
27	55538.0
28	74271.0
29	96519.0
30	121221.0
31	148514.0
32	179196.0
33	212719.0
34	254893.0
35	306827.0
36	372493.0
37	465658.0
38	639716.0
39	519845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61334582812249	20.79871141868668	13.008092398516355	25.579850354674477
2	19.96556629373317	22.939427239763994	32.11068113455382	24.98432533194902
3	20.888910986987216	23.95280295650083	30.022860295447828	25.135425761064123
4	15.906248630573355	16.366235529022855	34.232323300422145	33.49519253998164
5	14.922143530519532	37.26396702813911	33.33847395027478	14.475415491066562
6	35.01956805334107	35.77692419191187	15.05459166647003	14.148916088277034
7	28.98352867677711	31.56164639072509	20.89829331517544	18.55653161732236
8	27.905403659220358	33.20605390512725	19.705810638773624	19.182731796878773
9	27.803434044480102	13.153827484266342	18.832804664421648	40.20993380683191
10	16.86428546548146	26.300014270127903	32.6081411641503	24.227559100240345
11	37.759067430287054	21.299851343470742	20.62690806779097	20.31417315845123
12	21.785148055386074	25.083429684787482	28.65834954172664	24.473072718099804
13	30.48717317755298	18.89339652640367	24.490320471116476	26.12910982492688
14	23.12437708644439	20.473981736483548	24.00016405028928	32.40147712678279
15	25.887079466634304	27.82860340393114	21.17372363818596	25.11059349124859
16	25.573277106782133	25.908428476882957	23.54436268148063	24.973931734854283
17	24.505461413911238	25.729209154006146	24.41006504706333	25.35526438501929
18	24.647235696444042	24.656842751055933	25.84528035183169	24.850641200668335
19	25.259278111309246	25.098542536779288	25.595525022725457	24.04665432918601
20	24.974325006095704	24.133286365510788	25.090227659103498	25.80216096929001
21	25.97564976836324	25.055366972631692	25.077165435727682	23.891817823277385
22	25.975059861501105	24.47717397533178	24.615184090268112	24.932582072899002
23	24.520152903858666	24.41211567567932	25.34998331406304	25.717748106398975
24	24.655915754558293	25.056293969129328	25.26138492153115	25.02640535478122
25	25.353326119615133	24.220452127091782	24.713389537411892	25.712832215881193
26	24.694063064976273	25.50979189209536	25.21073720379653	24.585407839131836
27	26.07048431915197	24.764233890767226	24.410008865457414	24.755272924623387
28	24.525040703573488	25.54881001740506	25.10994740278054	24.81620187624091
29	24.59453735009343	25.566928585313452	25.36995587496671	24.468578189626403
30	25.02157373667232	25.612941320559884	24.98230279413599	24.38318214863181
31	25.147083444292008	25.535354522787824	24.62240342662851	24.695158606291663
32	24.750076126076017	25.1827025824437	24.3717491918276	25.695472099652683
33	24.116207157311866	24.806229641190555	25.19812443326805	25.87943876822953
34	25.392568971348506	25.0669403834507	25.479341461688076	24.06114918351272
35	25.551422462080225	24.794094414312376	25.47363902868745	24.180844094919944
36	24.075953036671983	25.71845037647294	25.39652977456569	24.80906681228939
37	24.66810716304239	25.41973277780961	25.57378274123539	24.338377317912606
38	24.440571659076532	24.521107991159262	25.96463817360341	25.073682176160794
39	24.59341371797508	24.63178575481673	25.772412808956695	25.002387718251494
40	24.936851874948733	24.462033032537015	26.303132349256327	24.297982743257926
41	23.68130534590453	24.820555950699514	26.7901706909551	24.707968012440855
42	24.603582588646148	24.67639394991522	26.57316923809877	24.14685422333986
43	23.79285392445372	23.80518578695261	26.671234231227757	25.730726057365917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	547.0
1	493.5
2	440.0
3	628.0
4	816.0
5	816.0
6	1435.0
7	2054.0
8	2036.0
9	2018.0
10	2756.0
11	3494.0
12	3494.0
13	6443.5
14	9393.0
15	15279.5
16	21166.0
17	18802.0
18	16438.0
19	16438.0
20	18646.0
21	20854.0
22	19459.0
23	18064.0
24	22280.0
25	26496.0
26	26496.0
27	32117.5
28	37739.0
29	43674.0
30	49609.0
31	57651.5
32	65694.0
33	65694.0
34	79083.0
35	92472.0
36	112059.0
37	131646.0
38	150168.5
39	168691.0
40	168691.0
41	186289.5
42	203888.0
43	215647.5
44	227407.0
45	248056.5
46	268706.0
47	268706.0
48	291144.5
49	313583.0
50	318362.5
51	323142.0
52	317065.0
53	310988.0
54	310988.0
55	297516.0
56	284044.0
57	273544.0
58	263044.0
59	243473.5
60	223903.0
61	223903.0
62	187057.5
63	150212.0
64	131848.0
65	113484.0
66	95553.0
67	77622.0
68	77622.0
69	64495.0
70	51368.0
71	43686.5
72	36005.0
73	26527.0
74	17049.0
75	17049.0
76	13485.0
77	9921.0
78	8716.5
79	7512.0
80	5865.0
81	4218.0
82	4218.0
83	3488.5
84	2759.0
85	2451.5
86	2144.0
87	1430.5
88	717.0
89	717.0
90	494.0
91	271.0
92	202.0
93	133.0
94	125.5
95	118.0
96	118.0
97	63.0
98	8.0
99	7.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3559884.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.271454265772476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37259596857136	40.907187367212174
2	9.572065288299306	9.624032847394536
3	3.2934736188831173	4.967031252216324
4	1.603156717939432	3.223720785070322
5	0.9170637217008705	2.30510634721422
6	0.6008290466322533	1.8122729963592603
7	0.45827664927661554	1.612676353062658
8	0.32073640086948824	1.2899108246143156
9	0.25680630553605555	1.1619023799526027
>10	1.4394982645746628	12.89523009600293
>50	0.0825520324562455	2.866220812181328
>100	0.06401261092102721	6.769551750447723
>500	0.012155450392444483	4.314282148819622
>1k	0.006553860345621108	5.458338781446178
>5k	1.6804770116977198E-4	0.5097243002118178
>10k+	5.6015900389923994E-5	0.2828109577938946
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10043	0.28211593411470715	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6405	0.17992159295078153	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6287	0.17660687820164928	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5409	0.15194315320386845	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	3875	0.10885186146514887	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGG	3764	0.10573378233672782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8090802958748093E-5
2	0.0	0.0	0.0	0.0	2.8090802958748093E-5
3	0.0	0.0	0.0	0.0	2.8090802958748093E-5
4	0.0	0.0	0.0	0.0	2.8090802958748093E-5
5	0.0	0.0	0.0	0.0	2.8090802958748093E-5
6	0.0	0.0	0.0	0.0	8.427240887624429E-5
7	0.0	0.0	0.0	0.0	8.427240887624429E-5
8	0.0	0.0	0.0	0.0	1.4045401479374046E-4
9	0.0	0.0	0.0	0.0	1.9663562071123666E-4
10	0.0	0.0	0.0	0.0	1.9663562071123666E-4
11	0.0	0.0	0.0	0.0	1.9663562071123666E-4
12	0.0	0.0	0.0	0.0	1.9663562071123666E-4
13	0.0	0.0	0.0	0.0	1.9663562071123666E-4
14	0.0	0.0	0.0	0.0	3.0899883254622906E-4
15	0.0	0.0	0.0	5.6181605917496186E-5	3.3708963550497714E-4
16	0.0	0.0	0.0	3.0899883254622906E-4	4.213620443812214E-4
17	0.0	0.0	0.0	4.213620443812214E-4	4.213620443812214E-4
18	0.0	0.0	0.0	4.213620443812214E-4	4.494528473399695E-4
19	0.0	0.0	0.0	5.337252562162138E-4	4.494528473399695E-4
20	0.0	0.0	0.0	6.179976650924581E-4	4.494528473399695E-4
21	0.0	0.0	0.0	8.70814891721191E-4	4.775436502987176E-4
22	0.0	0.0	0.0	0.0013764493449786566	5.337252562162138E-4
23	0.0	0.0	0.0	0.002134901024864855	7.303608769274505E-4
24	0.0	0.0	0.0	0.002865261901792306	7.303608769274505E-4
25	0.0	0.0	0.0	0.003314714749132275	7.303608769274505E-4
26	0.0	0.0	0.0	0.004859708911863421	7.865424828449466E-4
27	0.0	0.0	0.0	0.014045401479374046	8.146332858036948E-4
28	0.0	0.0	0.0	0.029270616683015515	8.146332858036948E-4
29	0.0	0.0	0.0	0.04969263043402538	8.146332858036948E-4
30	0.0	0.0	0.0	0.08289595953126562	8.146332858036948E-4
31	0.0	0.0	0.0	0.19992224465741018	8.427240887624428E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	1490	0.0	18.251677	4
AAGACGG	1440	0.0	18.243055	5
CTTATAC	1030	0.0	15.805826	37
ACGGACC	1555	0.0	15.704181	8
GACGGAC	1615	0.0	15.235294	7
TAGTACT	245	0.0	15.102041	4
CGGACCA	1610	0.0	15.052795	9
CGAACGA	840	0.0	14.9761915	16
CGCAAGA	1695	0.0	14.734514	2
ACGAACG	840	0.0	14.535715	15
TCTTATA	1605	0.0	14.523363	37
AGACGGA	1765	0.0	14.150143	6
AGAGCGA	1780	0.0	14.0309	15
GCGCAAG	1730	0.0	14.00867	1
TAATACC	270	0.0	13.703704	4
GTAAACG	855	0.0	13.6315775	27
CAGAGCG	1865	0.0	13.490618	14
GCAAGAC	2085	0.0	13.486811	3
AACGAAC	910	0.0	13.417583	14
ATACCGT	1230	0.0	13.386179	6
>>END_MODULE