FastQCFastQC Report
Thu 9 Feb 2017
SRR2727216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792231
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28670.1599682183825634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28190.1572899921940866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23830.1329627709820888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA450.003826630320.5555572
AAGACGG6550.019.7709925
CAAGACG6750.019.1851844
ACGGACC7100.018.58
CGCAAGA7500.017.7599982
TAGAGTG2850.016.8771935
GACGGAC7700.016.8181827
CGGACCA7700.016.577929
GGGTTAG1606.2937033E-1016.18751
GCGCAAG8000.016.1874981
TAAGACT2750.016.1454544
ATACCGT4150.016.0481936
TACCGTC4200.015.8571447
ACCGTCG4000.015.7249998
GTAAGAC1659.749783E-1015.696973
TTAGAGT3100.015.51612954
GGACCGT1557.215931E-915.51612956
TGTGCGA1101.4526902E-515.13636410
GCAAGAC9800.015.102043
GTATACT1353.975456E-715.0740754
Produced by FastQC (version 0.11.5)