##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727216.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1792231
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.51234857560214	31.0	30.0	31.0	27.0	34.0
2	30.7635645181899	31.0	31.0	33.0	27.0	34.0
3	30.856177021823637	31.0	30.0	34.0	27.0	34.0
4	34.707760327770245	35.0	35.0	37.0	32.0	37.0
5	34.42735060380051	35.0	35.0	37.0	30.0	37.0
6	34.53589074176264	35.0	35.0	37.0	30.0	37.0
7	34.49783035780544	35.0	35.0	37.0	30.0	37.0
8	34.50743124072734	35.0	35.0	37.0	30.0	37.0
9	36.01936859701679	37.0	35.0	39.0	31.0	39.0
10	35.684392246312	37.0	35.0	39.0	30.0	39.0
11	35.878647339544955	37.0	35.0	39.0	30.0	39.0
12	35.67716884709616	37.0	35.0	39.0	30.0	39.0
13	35.79313436716584	37.0	35.0	39.0	30.0	39.0
14	36.665529164488284	38.0	36.0	40.0	31.0	41.0
15	36.68060701996562	38.0	36.0	40.0	31.0	41.0
16	36.60705065362668	38.0	35.0	40.0	30.0	41.0
17	36.53110173855937	38.0	35.0	40.0	30.0	41.0
18	36.42779027926645	38.0	35.0	40.0	30.0	41.0
19	36.409812685976306	38.0	35.0	40.0	30.0	41.0
20	36.27975300058977	38.0	34.0	40.0	30.0	41.0
21	36.14812766881055	38.0	34.0	40.0	29.0	41.0
22	36.046472246044175	38.0	34.0	40.0	29.0	41.0
23	35.90113160636101	38.0	34.0	40.0	28.0	41.0
24	35.729440568765966	38.0	34.0	40.0	27.0	41.0
25	35.5862001047856	38.0	34.0	40.0	27.0	41.0
26	35.29557796958093	38.0	34.0	40.0	27.0	41.0
27	35.10011321085284	38.0	33.0	40.0	26.0	41.0
28	34.8531796403477	38.0	33.0	40.0	25.0	41.0
29	34.8007829347891	38.0	33.0	40.0	25.0	41.0
30	34.72250563682918	37.0	33.0	40.0	25.0	41.0
31	34.90928680510492	38.0	33.0	40.0	25.0	41.0
32	34.90533865333207	38.0	33.0	40.0	25.0	41.0
33	34.91312001633718	38.0	33.0	40.0	25.0	41.0
34	34.995593759956165	38.0	33.0	40.0	25.0	41.0
35	34.75246606045761	38.0	33.0	40.0	25.0	41.0
36	34.703963942148086	38.0	33.0	40.0	25.0	41.0
37	34.61320332033092	38.0	33.0	40.0	24.0	41.0
38	34.539672620326286	37.0	33.0	40.0	24.0	41.0
39	34.4574951554794	37.0	33.0	40.0	24.0	41.0
40	34.237854941689996	37.0	32.0	40.0	24.0	41.0
41	34.179885293804205	37.0	32.0	40.0	24.0	41.0
42	33.95458732719164	37.0	32.0	40.0	23.0	41.0
43	33.59822422444428	37.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	6.0
13	11.0
14	11.0
15	44.0
16	84.0
17	165.0
18	355.0
19	758.0
20	1443.0
21	2835.0
22	4855.0
23	8092.0
24	12525.0
25	18521.0
26	25938.0
27	34778.0
28	45300.0
29	57824.0
30	70979.0
31	85795.0
32	100937.0
33	119793.0
34	141929.0
35	167697.0
36	203185.0
37	253150.0
38	302317.0
39	132901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.6245411445288	23.60772690573927	14.103483312140009	23.664248637591918
2	23.26089661433152	23.76417995224946	29.881918123277636	23.093005310141383
3	24.04991320873258	24.011413707273224	28.614335986823125	23.324337097171068
4	19.465961698017722	17.97932297789738	31.960612220188132	30.594103103896764
5	15.337643417617484	37.82397469968994	32.92655913216544	13.911822750527136
6	35.3376322583417	36.418687100044586	14.327952144561722	13.915728497052
7	29.00011215072164	31.51033544225047	20.798156041269234	18.691396365758656
8	27.60001361431646	33.99411125016808	19.321170094703195	19.08470504081226
9	27.52435372449199	13.195285652351735	18.663777158189987	40.61658346496629
10	16.764691605044217	26.773334464139946	33.08892659484185	23.37304733597399
11	38.06629837336816	21.3531068260732	20.334320743252405	20.24627405730623
12	21.384073816377462	25.172759538251487	28.83618238943529	24.606984255935757
13	30.77064284682053	19.33696046993942	24.0872967826134	25.80509990062665
14	22.949999190952504	20.558064222748072	23.911147614342124	32.5807889719573
15	26.08659263231135	28.084884147188617	20.832415017930167	24.996108202569868
16	25.662763337984888	26.242710900547976	23.335663762093166	24.758861999373966
17	24.575626690979007	26.177708119098487	24.156037921450974	25.090627268471533
18	24.730126864226765	25.05977187092512	25.482652626809827	24.72744863803829
19	25.39773053808354	25.455423993893646	25.287644282461358	23.85920118556146
20	24.967652049317305	24.530877995079877	24.892103752250687	25.609366203352135
21	25.737697874883313	25.369274384830977	24.842277585869233	24.050750154416477
22	25.94743646326841	25.018761532414068	24.320469850147667	24.71333215416986
23	24.96787523483301	24.781850107491724	25.05603351353704	25.19424114413823
24	24.957943479384078	25.283068979389377	24.88072129094966	24.87826625027689
25	25.561492910233113	24.556823311280745	24.545775628253278	25.335908150232868
26	24.967763642075155	25.597481574640767	24.736097076771912	24.69865770651216
27	26.05194308099793	25.12187324067043	24.15804659109233	24.668137087239312
28	25.058265368694105	25.712645300745272	24.70987277867641	24.519216551884217
29	25.04186123328968	25.619800126211413	25.041693844152903	24.296644796346005
30	25.382721312152285	25.635646297826565	24.51157244797127	24.470059942049883
31	25.492193807606274	25.525336856688675	24.55129946976701	24.431169865938042
32	25.17370807669324	25.199151225483767	24.308919999709858	25.318220698113137
33	24.55938994471137	25.199486003757325	24.750771524429606	25.490352527101695
34	25.541573602956312	25.228611713557015	25.12750867494201	24.10230600854466
35	25.5436380689766	25.03488668592386	25.25528238268393	24.166192862415613
36	24.58890622916354	25.741882603302813	24.976133098914147	24.693078068619503
37	24.992927808971054	25.484493907314405	25.278270490801685	24.244307792912856
38	24.8585701285158	24.68309051679164	25.497382870846447	24.96095648384611
39	24.844900015678782	24.894781978439163	25.536719317989704	24.723598687892352
40	25.09536996068029	24.606872663177906	25.938006875229814	24.359750500911993
41	24.013589766051364	24.827603138211536	26.318314993993518	24.840492101743582
42	24.70546486474121	24.80372228803095	26.07917171391411	24.41164113331373
43	24.206422051621693	24.0071731824748	26.309610758880968	25.47679400702253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206.0
1	219.0
2	232.0
3	279.5
4	327.0
5	327.0
6	556.5
7	786.0
8	780.5
9	775.0
10	1121.5
11	1468.0
12	1468.0
13	2689.5
14	3911.0
15	5569.0
16	7227.0
17	6600.5
18	5974.0
19	5974.0
20	7521.5
21	9069.0
22	9101.5
23	9134.0
24	11289.0
25	13444.0
26	13444.0
27	16428.5
28	19413.0
29	22531.5
30	25650.0
31	30440.5
32	35231.0
33	35231.0
34	41518.5
35	47806.0
36	57295.0
37	66784.0
38	75406.0
39	84028.0
40	84028.0
41	93148.0
42	102268.0
43	109933.0
44	117598.0
45	128279.5
46	138961.0
47	138961.0
48	148978.5
49	158996.0
50	161001.0
51	163006.0
52	161130.5
53	159255.0
54	159255.0
55	151804.0
56	144353.0
57	137789.5
58	131226.0
59	121262.5
60	111299.0
61	111299.0
62	94292.0
63	77285.0
64	67329.0
65	57373.0
66	47878.5
67	38384.0
68	38384.0
69	31616.0
70	24848.0
71	20549.5
72	16251.0
73	12175.5
74	8100.0
75	8100.0
76	6315.5
77	4531.0
78	3803.5
79	3076.0
80	2404.0
81	1732.0
82	1732.0
83	1357.0
84	982.0
85	864.0
86	746.0
87	507.5
88	269.0
89	269.0
90	198.0
91	127.0
92	82.0
93	37.0
94	42.5
95	48.0
96	48.0
97	27.5
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1792231.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.33839170129137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.77187393709868	49.70888269230762
2	8.518397048907197	10.109359615103596
3	2.85580022422668	5.083757769773953
4	1.3985059287158836	3.319403703788854
5	0.8344471837965316	2.475737692307903
6	0.5379819001066587	1.9153788430040353
7	0.40758193454725944	1.6929679537774769
8	0.28760360950537656	1.3652748508428225
9	0.20735540630207505	1.1073722688479657
>10	1.0564677665217972	10.820464905651223
>50	0.06392373250806486	2.635666642473172
>100	0.054395687693617925	6.736325244936338
>500	0.003965948049141993	1.5501159187367965
>1k	0.001699692021060854	1.4792918984482257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2867	0.1599682183825634	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2819	0.1572899921940866	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2383	0.1329627709820888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	1.1159275785320085E-4
15	0.0	0.0	0.0	0.0	1.6738913677980126E-4
16	0.0	0.0	0.0	5.5796378926600423E-5	1.6738913677980126E-4
17	0.0	0.0	0.0	5.5796378926600423E-5	1.6738913677980126E-4
18	0.0	0.0	0.0	5.5796378926600423E-5	1.6738913677980126E-4
19	0.0	0.0	0.0	5.5796378926600423E-5	1.6738913677980126E-4
20	0.0	0.0	0.0	5.5796378926600423E-5	1.6738913677980126E-4
21	0.0	0.0	0.0	2.231855157064017E-4	1.6738913677980126E-4
22	0.0	0.0	0.0	3.9057465248620296E-4	1.6738913677980126E-4
23	0.0	0.0	0.0	7.811493049724059E-4	2.789818946330021E-4
24	0.0	0.0	0.0	0.0011717239574586089	2.789818946330021E-4
25	0.0	0.0	0.0	0.0020086696413576153	3.3477827355960253E-4
26	0.0	0.0	0.0	0.0031245972198896237	3.9057465248620296E-4
27	0.0	0.0	0.0	0.010433922859274278	3.9057465248620296E-4
28	0.0	0.0	0.0	0.022764922602052973	3.9057465248620296E-4
29	0.0	0.0	0.0	0.040619763858565104	3.9057465248620296E-4
30	0.0	0.0	0.0	0.06890852797435153	3.9057465248620296E-4
31	0.0	0.0	0.0	0.1596892364879304	3.9057465248620296E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	45	0.0038266303	20.555557	2
AAGACGG	655	0.0	19.770992	5
CAAGACG	675	0.0	19.185184	4
ACGGACC	710	0.0	18.5	8
CGCAAGA	750	0.0	17.759998	2
TAGAGTG	285	0.0	16.877193	5
GACGGAC	770	0.0	16.818182	7
CGGACCA	770	0.0	16.57792	9
GGGTTAG	160	6.2937033E-10	16.1875	1
GCGCAAG	800	0.0	16.187498	1
TAAGACT	275	0.0	16.145454	4
ATACCGT	415	0.0	16.048193	6
TACCGTC	420	0.0	15.857144	7
ACCGTCG	400	0.0	15.724999	8
GTAAGAC	165	9.749783E-10	15.69697	3
TTAGAGT	310	0.0	15.5161295	4
GGACCGT	155	7.215931E-9	15.5161295	6
TGTGCGA	110	1.4526902E-5	15.136364	10
GCAAGAC	980	0.0	15.10204	3
GTATACT	135	3.975456E-7	15.074075	4
>>END_MODULE