FastQCFastQC Report
Thu 9 Feb 2017
SRR2727215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279024
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38710.1180534207739864No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC36820.11228951053728181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.021.279791
CGGTATT650.001580778217.07692310
AAGACGG7950.016.2893075
TAAGACT3250.015.9384624
TATTCCG1201.938066E-615.4166675
TATACTG3850.015.3766255
CATATAA8100.015.30246937
TACTTAC11650.015.24463531
TATACCG1701.4897523E-915.2352945
TCTTATA15750.014.91746237
CTTATAC10050.014.91044737
CTTACAT10750.014.45581433
ACGAACG5300.014.31132115
TACATAT9450.014.29100535
GGGCGTC12300.014.28861811
TTACATA9800.014.15816334
CAAGACG9150.014.1530064
ACATATA8250.014.12727236
TCGTTAC14750.014.04745823
TACGACG7700.013.9350665
Produced by FastQC (version 0.11.5)