##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727215.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3279024
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214259486969294	31.0	31.0	34.0	30.0	34.0
2	31.468870615158657	31.0	31.0	34.0	30.0	34.0
3	31.62244863105607	31.0	31.0	34.0	30.0	34.0
4	35.42449704546231	37.0	35.0	37.0	33.0	37.0
5	35.236800035620355	37.0	35.0	37.0	33.0	37.0
6	35.22158392253305	37.0	35.0	37.0	32.0	37.0
7	35.13313168796569	37.0	35.0	37.0	32.0	37.0
8	35.132666610552405	37.0	35.0	37.0	32.0	37.0
9	36.71160656341643	39.0	35.0	39.0	32.0	39.0
10	36.43578028096165	38.0	35.0	39.0	32.0	39.0
11	36.60440972679675	39.0	35.0	39.0	32.0	39.0
12	36.44715927666281	38.0	35.0	39.0	32.0	39.0
13	36.51508863613075	38.0	35.0	39.0	32.0	39.0
14	37.544084459278125	39.0	36.0	41.0	32.0	41.0
15	37.561851941309364	39.0	36.0	41.0	32.0	41.0
16	37.49092595845593	39.0	36.0	41.0	32.0	41.0
17	37.44340724557063	39.0	36.0	41.0	32.0	41.0
18	37.3546350987367	39.0	36.0	41.0	32.0	41.0
19	37.382261307023064	39.0	36.0	41.0	32.0	41.0
20	37.25360412122632	39.0	36.0	40.0	31.0	41.0
21	37.149380425394874	39.0	36.0	40.0	31.0	41.0
22	37.081536152220906	39.0	36.0	40.0	31.0	41.0
23	36.97870250415978	39.0	36.0	40.0	31.0	41.0
24	36.851080382455265	39.0	36.0	40.0	30.0	41.0
25	36.687615888142325	39.0	35.0	40.0	30.0	41.0
26	36.48399096804415	38.0	35.0	40.0	30.0	41.0
27	36.333553520803754	38.0	35.0	40.0	30.0	41.0
28	36.08011987713417	38.0	35.0	40.0	29.0	41.0
29	36.05253758435437	38.0	35.0	40.0	29.0	41.0
30	36.00129855713163	38.0	34.0	40.0	29.0	41.0
31	36.16038674922782	38.0	35.0	40.0	29.0	41.0
32	36.16897772019967	38.0	35.0	40.0	29.0	41.0
33	36.2019344780642	38.0	35.0	40.0	30.0	41.0
34	36.2794371129946	38.0	35.0	40.0	30.0	41.0
35	36.09364310843714	38.0	35.0	40.0	29.0	41.0
36	36.0409792670014	38.0	35.0	40.0	29.0	41.0
37	36.019066649100466	38.0	35.0	40.0	28.0	41.0
38	35.945032424282346	38.0	34.0	40.0	28.0	41.0
39	35.85606967195117	38.0	34.0	40.0	28.0	41.0
40	35.73237432845871	38.0	34.0	40.0	27.0	41.0
41	35.680016980662536	38.0	34.0	40.0	27.0	41.0
42	35.52941789996048	38.0	34.0	40.0	27.0	41.0
43	35.18943899160238	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	6.0
15	13.0
16	41.0
17	94.0
18	202.0
19	421.0
20	956.0
21	1916.0
22	3813.0
23	6954.0
24	11404.0
25	18399.0
26	27228.0
27	39797.0
28	54815.0
29	73752.0
30	95866.0
31	120854.0
32	148803.0
33	180945.0
34	221967.0
35	270893.0
36	332873.0
37	420107.0
38	597758.0
39	649144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05961895978804	18.618375467822133	12.156818614319382	27.165186958070446
2	18.87259745582832	21.221345131966096	33.984014755610204	25.92204265659538
3	20.015163048516875	22.71788800569926	30.638720546113724	26.628228399670146
4	15.384333874957914	15.21727196873216	34.29929149649408	35.09910265981585
5	14.828009798037463	37.166730100176146	33.52854385939231	14.476716242394078
6	35.741397440213916	34.41670448279732	15.33986942456048	14.502028652428283
7	29.064898579577335	32.16774259657752	20.335654755805386	18.43170406803976
8	28.147948901868357	33.83329917682823	19.651365772254184	18.367386149049228
9	29.116102840357378	13.122929261877925	17.96647417036289	39.794493727401814
10	16.545777036093668	26.19721600085879	32.82821351719292	24.42879344585462
11	37.817564006850816	21.151293799618422	20.342486056826665	20.688656136704093
12	21.944060183761998	23.959537960075924	28.54648212394908	25.549919732213
13	30.980712553491525	18.818343507092354	23.872225393897697	26.32871854551842
14	23.070004977090743	20.167586452554175	23.795983194999486	32.9664253753556
15	26.90757981643318	27.279794231454236	20.972826060437498	24.839799891675085
16	25.271330737438944	25.932076129970383	23.775611279453887	25.020981853136785
17	24.854987337695608	24.801282332791708	23.865424589755975	26.478305739756706
18	24.433733940343224	25.764617764310355	25.243761558317352	24.557886737029065
19	26.17855191056851	24.808601583885938	25.114851248420262	23.89799525712529
20	24.789205568486235	23.780795748978964	24.74974260633652	26.680256076198283
21	25.47288461444625	25.131228072743596	25.81432767799199	23.581559634818166
22	26.568119050058797	23.518217615973533	23.918672141466484	25.994991192501182
23	24.838458028974475	24.59856957436115	25.36892075202865	25.194051644635724
24	24.291496494078725	24.145721409785352	25.515610742708805	26.047171353427117
25	25.768124905459672	23.21703043344605	24.451635608644523	26.563209052449754
26	24.799787985693303	24.53571550558947	25.44696226682086	25.217534241896367
27	26.91069049814823	24.296741957362922	24.10702086962462	24.685546674864227
28	24.271002591014888	26.28071645709211	23.552557102357284	25.895723849535717
29	24.91354134644943	25.608626225364624	24.989509073431606	24.48832335475434
30	26.76549485456648	24.562247790806044	23.9630755981048	24.70918175652267
31	25.55367084839879	24.984599075822562	24.8910956430938	24.570634432684848
32	25.348518339603494	24.63144521052606	24.084788644425903	25.935247805444543
33	24.349806527796076	24.149442029091585	24.80085537647788	26.69989606663446
34	25.70063531099498	24.700825611523427	25.7038374833487	23.89470159413289
35	25.701733198659117	24.08015311873289	25.06129872791416	25.156814954693836
36	24.490031180009662	24.611774723210324	25.85217430552506	25.04601979125496
37	24.860263297859365	24.815829344341488	26.091513816306318	24.232393541492833
38	25.545894144111177	23.723278634130157	25.22457902107456	25.506248200684105
39	23.992779558795544	24.615525839396113	26.38712006987445	25.004574531933894
40	25.97547318958324	23.1892782730471	26.069342584866718	24.76590595250294
41	23.96396000761202	24.524096194477384	26.465619037860044	25.046324760050553
42	24.55678884936493	24.25639458570599	26.430852595162463	24.755963969766615
43	23.69113492307467	23.36039626425424	26.043359243482207	26.90510956918888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	300.0
1	333.5
2	367.0
3	570.0
4	773.0
5	773.0
6	1039.0
7	1305.0
8	1256.0
9	1207.0
10	1552.0
11	1897.0
12	1897.0
13	3199.0
14	4501.0
15	6569.5
16	8638.0
17	8351.5
18	8065.0
19	8065.0
20	9729.5
21	11394.0
22	11769.5
23	12145.0
24	15459.0
25	18773.0
26	18773.0
27	23447.5
28	28122.0
29	33497.5
30	38873.0
31	46850.5
32	54828.0
33	54828.0
34	65348.5
35	75869.0
36	91338.5
37	106808.0
38	122503.0
39	138198.0
40	138198.0
41	154668.5
42	171139.0
43	185539.5
44	199940.0
45	226688.5
46	253437.0
47	253437.0
48	282981.0
49	312525.0
50	328949.0
51	345373.0
52	339062.0
53	332751.0
54	332751.0
55	309980.5
56	287210.0
57	270484.5
58	253759.0
59	228929.0
60	204099.0
61	204099.0
62	171984.5
63	139870.0
64	121097.5
65	102325.0
66	84266.5
67	66208.0
68	66208.0
69	53792.5
70	41377.0
71	33716.5
72	26056.0
73	19614.0
74	13172.0
75	13172.0
76	10252.0
77	7332.0
78	6077.0
79	4822.0
80	3678.0
81	2534.0
82	2534.0
83	2001.5
84	1469.0
85	1224.0
86	979.0
87	660.0
88	341.0
89	341.0
90	236.5
91	132.0
92	95.0
93	58.0
94	52.0
95	46.0
96	46.0
97	25.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3279024.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.13918150527665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56927092073809	41.20246569363433
2	9.74027412168438	9.96219292439933
3	3.373856346490453	5.176087562277145
4	1.7048995640510003	3.487486730170845
5	1.0189452030870898	2.605401184230083
6	0.6808989444822798	2.089236882517836
7	0.5090065917963258	1.8221126339678195
8	0.3619960460792724	1.4809745203712321
9	0.2784541892606113	1.2815927392952748
>10	1.584575365207776	14.523267457539383
>50	0.09995852776975127	3.5202817124232095
>100	0.06661566264914415	7.271398005184156
>500	0.007897894707650796	2.719752782720764
>1k	0.0033506219961466975	2.8577491712684933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3871	0.1180534207739864	No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACC	3682	0.11228951053728181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0496879559283494E-5
2	0.0	0.0	0.0	0.0	6.099375911856699E-5
3	0.0	0.0	0.0	0.0	6.099375911856699E-5
4	0.0	0.0	0.0	0.0	6.099375911856699E-5
5	0.0	0.0	0.0	0.0	6.099375911856699E-5
6	0.0	0.0	0.0	0.0	9.149063867785048E-5
7	0.0	0.0	0.0	0.0	9.149063867785048E-5
8	0.0	0.0	0.0	0.0	9.149063867785048E-5
9	0.0	0.0	0.0	0.0	1.2198751823713397E-4
10	0.0	0.0	0.0	0.0	1.2198751823713397E-4
11	0.0	0.0	0.0	0.0	1.2198751823713397E-4
12	0.0	0.0	0.0	0.0	1.5248439779641748E-4
13	0.0	0.0	0.0	0.0	1.8298127735570096E-4
14	0.0	0.0	0.0	0.0	3.354656751521184E-4
15	0.0	0.0	0.0	0.0	3.354656751521184E-4
16	0.0	0.0	0.0	9.149063867785048E-5	4.2695631382996894E-4
17	0.0	0.0	0.0	2.7447191603355146E-4	4.2695631382996894E-4
18	0.0	0.0	0.0	3.659625547114019E-4	4.574531933892524E-4
19	0.0	0.0	0.0	3.9645943427068543E-4	4.574531933892524E-4
20	0.0	0.0	0.0	5.184469525078194E-4	4.879500729485359E-4
21	0.0	0.0	0.0	6.099375911856699E-4	4.879500729485359E-4
22	0.0	0.0	0.0	9.454032663377883E-4	4.879500729485359E-4
23	0.0	0.0	0.0	0.0015248439779641746	5.794407116263864E-4
24	0.0	0.0	0.0	0.002592234762539097	6.099375911856699E-4
25	0.0	0.0	0.0	0.003385153631080468	6.099375911856699E-4
26	0.0	0.0	0.0	0.004970991368163209	6.099375911856699E-4
27	0.0	0.0	0.0	0.013723595801677573	6.099375911856699E-4
28	0.0	0.0	0.0	0.02888054494264147	6.099375911856699E-4
29	0.0	0.0	0.0	0.050167366875021345	6.099375911856699E-4
30	0.0	0.0	0.0	0.08337846871508107	6.099375911856699E-4
31	0.0	0.0	0.0	0.2200654828997897	6.099375911856699E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1930	0.0	21.27979	1
CGGTATT	65	0.0015807782	17.076923	10
AAGACGG	795	0.0	16.289307	5
TAAGACT	325	0.0	15.938462	4
TATTCCG	120	1.938066E-6	15.416667	5
TATACTG	385	0.0	15.376625	5
CATATAA	810	0.0	15.302469	37
TACTTAC	1165	0.0	15.244635	31
TATACCG	170	1.4897523E-9	15.235294	5
TCTTATA	1575	0.0	14.917462	37
CTTATAC	1005	0.0	14.910447	37
CTTACAT	1075	0.0	14.455814	33
ACGAACG	530	0.0	14.311321	15
TACATAT	945	0.0	14.291005	35
GGGCGTC	1230	0.0	14.288618	11
TTACATA	980	0.0	14.158163	34
CAAGACG	915	0.0	14.153006	4
ACATATA	825	0.0	14.127272	36
TCGTTAC	1475	0.0	14.047458	23
TACGACG	770	0.0	13.935066	5
>>END_MODULE