##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727214.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3287020
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97491192630407	31.0	31.0	33.0	28.0	34.0
2	31.229034201191354	31.0	31.0	34.0	28.0	34.0
3	31.381454934865015	31.0	31.0	34.0	28.0	34.0
4	35.21865397837555	35.0	35.0	37.0	33.0	37.0
5	35.00291844892943	35.0	35.0	37.0	32.0	37.0
6	35.01071395975686	36.0	35.0	37.0	32.0	37.0
7	34.930619831945044	36.0	35.0	37.0	32.0	37.0
8	34.91822806067502	36.0	35.0	37.0	32.0	37.0
9	36.4746043528789	38.0	35.0	39.0	32.0	39.0
10	36.178445522083834	38.0	35.0	39.0	32.0	39.0
11	36.34266782678536	38.0	35.0	39.0	32.0	39.0
12	36.169392337132116	38.0	35.0	39.0	31.0	39.0
13	36.250900815936625	38.0	35.0	39.0	32.0	39.0
14	37.23770466866645	39.0	36.0	40.0	32.0	41.0
15	37.24604687528521	39.0	36.0	40.0	32.0	41.0
16	37.18992004916307	39.0	36.0	40.0	31.0	41.0
17	37.121274284914605	39.0	36.0	40.0	31.0	41.0
18	37.03343240990319	39.0	36.0	40.0	31.0	41.0
19	37.03660853904144	39.0	36.0	40.0	31.0	41.0
20	36.91838565022422	39.0	36.0	40.0	31.0	41.0
21	36.79808184921296	39.0	36.0	40.0	30.0	41.0
22	36.7181416602272	39.0	35.0	40.0	30.0	41.0
23	36.60499996957731	38.0	35.0	40.0	30.0	41.0
24	36.45425217978595	38.0	35.0	40.0	30.0	41.0
25	36.31030416608357	38.0	35.0	40.0	30.0	41.0
26	36.06748696387609	38.0	34.0	40.0	29.0	41.0
27	35.90587157972875	38.0	34.0	40.0	27.0	41.0
28	35.664047069990445	38.0	34.0	40.0	27.0	41.0
29	35.62159980772858	38.0	34.0	40.0	27.0	41.0
30	35.55425491782831	38.0	34.0	40.0	27.0	41.0
31	35.74497234577216	38.0	34.0	40.0	27.0	41.0
32	35.7506756880092	38.0	34.0	40.0	27.0	41.0
33	35.76710363794562	38.0	34.0	40.0	27.0	41.0
34	35.840289684881746	38.0	34.0	40.0	28.0	41.0
35	35.63281817573365	38.0	34.0	40.0	27.0	41.0
36	35.59940036872304	38.0	34.0	40.0	27.0	41.0
37	35.544408917499744	38.0	34.0	40.0	27.0	41.0
38	35.464586160108546	38.0	34.0	40.0	26.0	41.0
39	35.4012798826901	38.0	34.0	40.0	26.0	41.0
40	35.233269344269274	38.0	33.0	40.0	26.0	41.0
41	35.18635085883262	38.0	33.0	40.0	26.0	41.0
42	34.99678280022634	38.0	33.0	40.0	25.0	41.0
43	34.66195064222305	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	7.0
15	26.0
16	63.0
17	136.0
18	292.0
19	642.0
20	1444.0
21	2913.0
22	5331.0
23	9407.0
24	15129.0
25	23364.0
26	34231.0
27	48196.0
28	65431.0
29	85675.0
30	109221.0
31	134017.0
32	162053.0
33	194268.0
34	234399.0
35	280460.0
36	343746.0
37	428305.0
38	601350.0
39	506909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2130136111128	19.86315872735791	12.496121106655876	26.42770655487341
2	19.40934341744194	22.095150014298664	33.00338908798851	25.49211748027088
3	20.459899848494988	23.475853508649173	30.082932260832003	25.98131438202384
4	15.62409112204976	15.954603257661956	34.49769091760926	33.923614702679025
5	14.703013671958187	37.38319207062932	33.6613406672305	14.252453590181988
6	35.281622868129794	35.76315325127319	15.122177534666658	13.833046345930356
7	29.47408290792268	31.700719800913895	20.524669761668623	18.3005275294948
8	28.119147434454305	33.889997627029956	19.438275398385162	18.552579540130573
9	28.33347530590018	13.19785094097389	17.93204787314954	40.53662587997639
10	16.484140650193794	26.236195703098858	32.83582089552239	24.443842751184963
11	38.276767406343744	20.965890076726033	20.627772267890066	20.129570249040164
12	21.99679344816886	24.37393140291206	28.963590121143163	24.66568502777592
13	30.632213981052747	18.822976434582085	24.41810515299572	26.126704431369447
14	22.931135192362685	19.770034864406057	24.1490164343387	33.14981350889255
15	26.04584091365431	27.406647966851434	21.171912553011545	25.37559856648271
16	25.616029108432564	25.839179560818003	23.45975990410767	25.085031426641763
17	24.679679466507658	25.55244568028184	24.107824108158756	25.66005074505175
18	24.805203497392775	25.117066522260284	25.291418975242014	24.786311005104928
19	25.793515098782482	24.954670187586323	25.10997803481573	24.141836678815462
20	25.223180875078338	23.93879562643367	24.68196116847479	26.156062330013203
21	25.946480398658966	24.893976915260634	25.026954505905046	24.132588180175357
22	26.369690479522482	24.071316876684655	24.177644188352975	25.381348455439884
23	24.97952552768161	24.361579789596654	25.033069467176954	25.62582521554478
24	24.73127635365772	24.506087580848305	25.09829572074402	25.66434034474996
25	25.599448740804743	23.69550535135168	24.627200321263636	26.07784558657994
26	24.916276749152726	24.916033367609565	25.157863353432592	25.009826529805114
27	26.400843317047052	24.45160053787321	24.171863876702908	24.97569226837683
28	24.80422997122013	25.521384110835953	24.468241750886822	25.206144167057094
29	25.058624529208828	25.253086382194205	24.933039652937918	24.755249435659046
30	25.801607535092575	25.142165243898727	24.438640470699905	24.61758675030879
31	25.399297844247982	25.05908086960225	24.535171675256006	25.00644961089376
32	25.331151012162994	24.852723743694895	24.063224440374565	25.75290080376755
33	24.59209253366271	24.483939860420683	24.783420849279896	26.140546756636713
34	25.732091681827306	24.681170178459517	25.31606135648703	24.270676783226143
35	25.699995740822995	24.291577173245066	25.244598450876477	24.76382863505546
36	24.599059330335685	25.01676290378519	25.295069698389423	25.0891080674897
37	25.106114352818054	24.858473632652068	25.48575913745581	24.549652877074067
38	25.17991980578153	24.08056537532476	25.35950496194121	25.380009856952494
39	24.774689536419007	24.47791616722746	25.64359206819551	25.103802228158028
40	25.587857694811717	23.870314144726834	25.89990325583659	24.641924904624858
41	24.07271632055783	24.55695432336889	26.2662533236792	25.104076032394083
42	24.800457557301144	24.251997249788563	26.2569439796533	24.690601213256993
43	24.13794257412489	23.301805282596394	26.202578627449785	26.357673515828928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	162.0
1	184.0
2	206.0
3	339.5
4	473.0
5	473.0
6	803.5
7	1134.0
8	1092.0
9	1050.0
10	1398.5
11	1747.0
12	1747.0
13	3052.0
14	4357.0
15	7027.5
16	9698.0
17	9078.0
18	8458.0
19	8458.0
20	10179.0
21	11900.0
22	12174.0
23	12448.0
24	16016.0
25	19584.0
26	19584.0
27	24352.0
28	29120.0
29	34969.5
30	40819.0
31	49370.5
32	57922.0
33	57922.0
34	68934.5
35	79947.0
36	97315.5
37	114684.0
38	132182.0
39	149680.0
40	149680.0
41	167266.5
42	184853.0
43	198249.5
44	211646.0
45	233620.5
46	255595.0
47	255595.0
48	279533.0
49	303471.0
50	311132.5
51	318794.0
52	313638.5
53	308483.0
54	308483.0
55	293822.5
56	279162.0
57	263748.5
58	248335.0
59	228350.0
60	208365.0
61	208365.0
62	175885.0
63	143405.0
64	125340.5
65	107276.0
66	88980.0
67	70684.0
68	70684.0
69	57248.0
70	43812.0
71	35870.5
72	27929.0
73	20691.0
74	13453.0
75	13453.0
76	10327.5
77	7202.0
78	6126.5
79	5051.0
80	3876.5
81	2702.0
82	2702.0
83	2140.0
84	1578.0
85	1355.0
86	1132.0
87	774.5
88	417.0
89	417.0
90	275.0
91	133.0
92	100.0
93	67.0
94	71.5
95	76.0
96	76.0
97	41.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3287020.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.48326969180994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4469969524834	41.41674440000915
2	9.905435749948403	10.199284402589786
3	3.4400390460055474	5.3131337386758055
4	1.7413190832243741	3.585951999245427
5	0.9850724388190832	2.53573750168459
6	0.6656578137553035	2.056214444881493
7	0.4541074016364644	1.6365253679248009
8	0.35541628424808	1.463839393184391
9	0.27086889417725285	1.255069469704485
>10	1.5711499451923432	14.583751803015351
>50	0.09172071794627677	3.2297186374510756
>100	0.05944198790847642	6.277409282769857
>500	0.009446585396650442	3.338594032574966
>1k	0.0033270992582399945	3.108025526288733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4661	0.14180017158398792	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4088	0.12436796855510462	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3343	0.10170306234826682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.12680786852529E-5
2	0.0	0.0	0.0	0.0	9.12680786852529E-5
3	0.0	0.0	0.0	0.0	9.12680786852529E-5
4	0.0	0.0	0.0	0.0	1.521134644754215E-4
5	0.0	0.0	0.0	0.0	1.521134644754215E-4
6	0.0	0.0	0.0	0.0	2.129588502655901E-4
7	0.0	0.0	0.0	0.0	2.129588502655901E-4
8	0.0	0.0	0.0	0.0	2.129588502655901E-4
9	0.0	0.0	0.0	3.04226928950843E-5	3.954950076360959E-4
10	0.0	0.0	0.0	3.04226928950843E-5	3.954950076360959E-4
11	0.0	0.0	0.0	3.04226928950843E-5	3.954950076360959E-4
12	0.0	0.0	0.0	3.04226928950843E-5	3.954950076360959E-4
13	0.0	0.0	0.0	3.04226928950843E-5	4.563403934262645E-4
14	0.0	0.0	0.0	3.04226928950843E-5	5.780311650066017E-4
15	0.0	0.0	0.0	6.08453857901686E-5	7.301446294820232E-4
16	0.0	0.0	0.0	9.12680786852529E-5	8.214127081672761E-4
17	0.0	0.0	0.0	1.216907715803372E-4	8.214127081672761E-4
18	0.0	0.0	0.0	1.521134644754215E-4	9.12680786852529E-4
19	0.0	0.0	0.0	1.521134644754215E-4	9.12680786852529E-4
20	0.0	0.0	0.0	3.04226928950843E-4	9.12680786852529E-4
21	0.0	0.0	0.0	4.867630863213488E-4	9.735261726426976E-4
22	0.0	0.0	0.0	7.909900152721918E-4	9.735261726426976E-4
23	0.0	0.0	0.0	0.001308175794488625	0.0011256396371181192
24	0.0	0.0	0.0	0.0019470523452853952	0.0011256396371181192
25	0.0	0.0	0.0	0.0021295885026559012	0.0011256396371181192
26	0.0	0.0	0.0	0.0030726919824035142	0.0011256396371181192
27	0.0	0.0	0.0	0.00982652980511223	0.0011560623300132034
28	0.0	0.0	0.0	0.023303782757634575	0.0011560623300132034
29	0.0	3.04226928950843E-5	0.0	0.04307853313943937	0.0011560623300132034
30	0.0	3.04226928950843E-5	0.0	0.07337953526294333	0.0011560623300132034
31	0.0	3.04226928950843E-5	0.0	0.19075028445217856	0.0011864850229082878
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1025	0.0	18.048782	5
GGTATCA	2275	0.0	17.80879	1
CAAGACG	1080	0.0	17.643518	4
ATACCGT	805	0.0	15.857143	6
GACGGAC	1135	0.0	15.810573	7
ACGGACC	1145	0.0	15.672488	8
TCGTTAC	860	0.0	15.058139	23
TACCGTC	770	0.0	14.896105	7
ACCGTCG	800	0.0	14.799999	8
CGGACCA	1245	0.0	14.413654	9
AGACGGA	1310	0.0	14.40458	6
TCGTTTA	610	0.0	14.254099	30
CGCAAGA	1380	0.0	13.807971	2
CCGTCGT	870	0.0	13.609196	9
CTTATAC	890	0.0	13.511235	37
GCGCAAG	1385	0.0	13.490974	1
CTGTGCG	510	0.0	13.421569	9
CGTCGTA	855	0.0	13.415205	10
TACGGAT	1610	0.0	13.329193	27
GAGCGAA	1320	0.0	13.314394	16
>>END_MODULE