##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727213.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3048357
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97010947208611	31.0	31.0	33.0	28.0	34.0
2	31.224357908210884	31.0	31.0	34.0	28.0	34.0
3	31.37442924171939	31.0	31.0	34.0	28.0	34.0
4	35.21314924728304	35.0	35.0	37.0	33.0	37.0
5	35.0001879701098	35.0	35.0	37.0	32.0	37.0
6	35.00220085770794	36.0	35.0	37.0	32.0	37.0
7	34.92192449900061	36.0	35.0	37.0	32.0	37.0
8	34.91313878262946	36.0	35.0	37.0	32.0	37.0
9	36.46260231331173	38.0	35.0	39.0	32.0	39.0
10	36.171697081411395	38.0	35.0	39.0	32.0	39.0
11	36.337377479081354	38.0	35.0	39.0	32.0	39.0
12	36.164690684194795	38.0	35.0	39.0	31.0	39.0
13	36.24543811633611	38.0	35.0	39.0	32.0	39.0
14	37.22284463401104	39.0	36.0	40.0	31.0	41.0
15	37.23950672444205	39.0	36.0	40.0	31.0	41.0
16	37.17957542374466	39.0	36.0	40.0	31.0	41.0
17	37.12635527925371	39.0	36.0	40.0	31.0	41.0
18	37.04065435905309	39.0	36.0	40.0	31.0	41.0
19	37.056770253615305	39.0	36.0	40.0	31.0	41.0
20	36.937534875344326	39.0	36.0	40.0	31.0	41.0
21	36.82402618853369	39.0	36.0	40.0	30.0	41.0
22	36.745749923647395	39.0	35.0	40.0	30.0	41.0
23	36.63384111506625	38.0	35.0	40.0	30.0	41.0
24	36.49155331872218	38.0	35.0	40.0	30.0	41.0
25	36.34351061899902	38.0	35.0	40.0	30.0	41.0
26	36.10770424855094	38.0	34.0	40.0	29.0	41.0
27	35.947086578114046	38.0	34.0	40.0	28.0	41.0
28	35.70974233004861	38.0	34.0	40.0	27.0	41.0
29	35.671327866125914	38.0	34.0	40.0	27.0	41.0
30	35.617148844443086	38.0	34.0	40.0	27.0	41.0
31	35.78219939462471	38.0	34.0	40.0	27.0	41.0
32	35.799926649011255	38.0	34.0	40.0	28.0	41.0
33	35.81894148224765	38.0	34.0	40.0	28.0	41.0
34	35.89601250772137	38.0	34.0	40.0	28.0	41.0
35	35.69056872275787	38.0	34.0	40.0	27.0	41.0
36	35.655760463751456	38.0	34.0	40.0	27.0	41.0
37	35.60549010499754	38.0	34.0	40.0	27.0	41.0
38	35.54061614174455	38.0	34.0	40.0	27.0	41.0
39	35.475557160791865	38.0	34.0	40.0	26.0	41.0
40	35.309790487137825	38.0	33.0	40.0	26.0	41.0
41	35.268335040810506	38.0	33.0	40.0	26.0	41.0
42	35.08279509256954	38.0	33.0	40.0	25.0	41.0
43	34.76114083750689	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	7.0
15	15.0
16	60.0
17	123.0
18	231.0
19	577.0
20	1199.0
21	2572.0
22	4760.0
23	8484.0
24	13740.0
25	21276.0
26	31296.0
27	44257.0
28	60078.0
29	78850.0
30	100311.0
31	123478.0
32	150151.0
33	179664.0
34	216026.0
35	260696.0
36	317485.0
37	394114.0
38	553435.0
39	485468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43473254608958	20.287321990173723	13.066087731850304	26.21185773188639
2	18.923242914133745	22.09019481642078	34.04253504428779	24.944027225157683
3	20.191696707439448	23.611866982771375	30.568040423086927	25.62839588670225
4	15.47614009776414	16.32390825615241	34.758756930372655	33.44119471571079
5	14.439975370338843	37.65339820762463	33.90521517000798	14.00141125202855
6	34.763677613875274	36.107057014647566	15.304703484532817	13.824561886944345
7	28.809191311909988	32.198951763195716	20.819510313260555	18.172346611633742
8	27.598243906471588	34.829352336356926	19.53183304973794	18.040570707433545
9	28.30741281286936	13.566422830396832	18.327905819429944	39.798258537303866
10	16.293728064003002	26.87985691964557	33.02592183264624	23.80049318370519
11	37.76624588261808	21.410746838378838	20.841522170795614	19.98148510820747
12	21.66557263470125	24.690513611102638	29.2723260431767	24.37158771101941
13	30.463820346501407	19.300921775238265	24.520618812035465	25.714639066224855
14	22.60988460341095	20.199996260280535	24.645833804898835	32.544285331409675
15	25.877776126615093	27.807176127992882	21.50778271705053	24.807265028341497
16	24.971681466442412	26.42430004097289	24.0377357376449	24.56628275493979
17	24.281801639374915	25.935249709925706	24.493751880111155	25.289196770588223
18	24.219243349778257	25.782282062107555	25.760401422799234	24.238073165314955
19	25.42999392787656	25.42280972996273	25.623475203199625	23.523721138961086
20	24.704291524909976	24.402259971519083	25.194850865564632	25.69859763800631
21	25.441245890819218	25.40844133413508	25.639090172181277	23.51122260286443
22	25.98826187352728	24.449301705804142	24.566774823290054	24.995661597378522
23	24.546796848269413	24.915684088182584	25.513547133751068	25.023971929796936
24	24.25719822186181	24.82862079474287	25.678291617418825	25.235889365976494
25	25.14026408324222	24.165411072259584	25.002648967952247	25.691675876545954
26	24.426600952578717	25.27039975960821	25.695382791451266	24.60761649636181
27	25.925178711023676	24.91643859298632	24.748643285546937	24.40973941044307
28	24.33806145408822	26.169703876547267	24.67499705579104	24.817237613573475
29	24.55637577882118	25.860455320685865	25.482087563890975	24.10108133660198
30	25.669565605340843	25.44364062345716	24.719676861994838	24.167116909207156
31	24.931528689061025	25.53818335582086	25.170411470834946	24.35987648428317
32	24.912174000617384	25.30231859326188	24.607026014341496	25.178481391779243
33	24.147040520516462	24.850763870504668	25.230214177670135	25.77198143130873
34	25.185304739569546	25.145709639651788	25.971728376958474	23.697257243820196
35	25.238054466717646	24.599284138964038	25.720806322881472	24.441855071436844
36	24.133196997595753	25.18136819276745	26.04671959353842	24.63871521609838
37	24.502149846622295	25.07104646863868	26.20516560232283	24.2216380824162
38	24.706325407424394	24.26067550487033	25.931280358566926	25.10171872913835
39	24.039179138139005	24.742082374210106	26.51680889082217	24.701929596828716
40	24.96895868823763	24.01306671101843	26.66557755538475	24.35239704535919
41	23.535661997594115	24.677621420325767	27.1011236544801	24.685592927600013
42	23.98941462564916	24.586719993754013	27.041911429665227	24.3819539509316
43	23.316593168057416	23.696240302562988	26.897341748358215	26.08982478102138
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	258.0
2	280.0
3	452.5
4	625.0
5	625.0
6	986.5
7	1348.0
8	1318.5
9	1289.0
10	1761.0
11	2233.0
12	2233.0
13	3790.5
14	5348.0
15	8406.0
16	11464.0
17	10965.5
18	10467.0
19	10467.0
20	12407.0
21	14347.0
22	15112.0
23	15877.0
24	19865.5
25	23854.0
26	23854.0
27	29252.0
28	34650.0
29	40775.0
30	46900.0
31	55477.5
32	64055.0
33	64055.0
34	75481.5
35	86908.0
36	101784.5
37	116661.0
38	131161.0
39	145661.0
40	145661.0
41	160998.5
42	176336.0
43	188570.0
44	200804.0
45	220268.0
46	239732.0
47	239732.0
48	260033.5
49	280335.0
50	285804.0
51	291273.0
52	286976.5
53	282680.0
54	282680.0
55	266195.0
56	249710.0
57	233199.0
58	216688.0
59	196877.0
60	177066.0
61	177066.0
62	148839.5
63	120613.0
64	104777.5
65	88942.0
66	73565.0
67	58188.0
68	58188.0
69	46823.5
70	35459.0
71	28959.0
72	22459.0
73	16647.5
74	10836.0
75	10836.0
76	8371.0
77	5906.0
78	5008.5
79	4111.0
80	3208.0
81	2305.0
82	2305.0
83	1768.5
84	1232.0
85	1063.0
86	894.0
87	617.0
88	340.0
89	340.0
90	237.0
91	134.0
92	94.5
93	55.0
94	54.0
95	53.0
96	53.0
97	27.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3048357.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.16129770546989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.46501512948804	44.93715952138299
2	9.797400609057444	10.808746634719393
3	3.2272362928115514	5.340556257410251
4	1.5976776044151733	3.5251987989802926
5	0.9075402019762165	2.503054763044618
6	0.5975963220588267	1.9778513177268482
7	0.4245503030139818	1.639312195885119
8	0.3127423086830774	1.3801017275490557
9	0.23134547840649908	1.1485185126475725
>10	1.2985378204286078	12.745193588831869
>50	0.07657001590256174	2.8905428765779484
>100	0.05433394216882805	6.174628546729776
>500	0.0070007257973076	2.5643279853384984
>1k	0.002393410528687184	2.1906020924614267
>5k	5.9835263217179605E-5	0.17420518071435004
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5278	0.17314245017889965	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3816	0.12518218830668454	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3324	0.10904234641808686	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3320	0.10891112819135029	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	3.2804556684141655E-5
6	0.0	0.0	0.0	0.0	6.560911336828331E-5
7	0.0	0.0	0.0	0.0	6.560911336828331E-5
8	0.0	0.0	0.0	0.0	6.560911336828331E-5
9	0.0	0.0	0.0	3.2804556684141655E-5	9.841367005242496E-5
10	0.0	0.0	0.0	3.2804556684141655E-5	9.841367005242496E-5
11	0.0	0.0	0.0	3.2804556684141655E-5	1.3121822673656662E-4
12	0.0	0.0	0.0	3.2804556684141655E-5	1.3121822673656662E-4
13	0.0	0.0	0.0	6.560911336828331E-5	1.3121822673656662E-4
14	0.0	0.0	0.0	6.560911336828331E-5	1.9682734010484991E-4
15	0.0	0.0	0.0	6.560911336828331E-5	1.9682734010484991E-4
16	0.0	0.0	0.0	9.841367005242496E-5	1.9682734010484991E-4
17	0.0	0.0	0.0	1.3121822673656662E-4	2.296318967889916E-4
18	0.0	0.0	0.0	1.3121822673656662E-4	2.6243645347313324E-4
19	0.0	0.0	0.0	1.3121822673656662E-4	2.6243645347313324E-4
20	0.0	0.0	0.0	1.6402278342070827E-4	2.6243645347313324E-4
21	0.0	0.0	0.0	1.6402278342070827E-4	3.2804556684141653E-4
22	0.0	0.0	0.0	2.952410101572749E-4	3.2804556684141653E-4
23	0.0	0.0	0.0	5.576774636304081E-4	3.608501235255582E-4
24	0.0	0.0	0.0	0.0011153549272608161	3.608501235255582E-4
25	0.0	0.0	0.0	0.0015418141641546578	3.608501235255582E-4
26	0.0	0.0	0.0	0.002952410101572749	3.608501235255582E-4
27	0.0	0.0	0.0	0.010399044468872904	3.608501235255582E-4
28	0.0	0.0	0.0	0.02837594153178253	3.608501235255582E-4
29	0.0	0.0	0.0	0.056095791929882226	3.608501235255582E-4
30	0.0	0.0	0.0	0.09920097941284436	3.608501235255582E-4
31	0.0	0.0	0.0	0.29776696102195377	3.608501235255582E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2225	0.0	20.370787	1
AAGACGG	850	0.0	16.32353	5
CTTATAC	1280	0.0	16.042969	37
ACCGTCG	560	0.0	15.857142	8
TACCGTC	590	0.0	15.677966	7
TACTTAC	1040	0.0	15.653846	31
GACGGTA	700	0.0	15.592856	8
CAAGACG	905	0.0	15.535912	4
CGACGGT	720	0.0	15.416666	7
TCTTATA	2065	0.0	15.319612	37
CCGTCGT	570	0.0	15.254386	9
TTACGCC	85	5.3677615E-4	15.235294	4
TATACCG	170	1.4897523E-9	15.235294	5
GTGCGTA	135	3.9781116E-7	15.074075	15
TACGACG	725	0.0	15.055173	5
AGTGTAC	1095	0.0	15.0365305	27
GTACTTA	1160	0.0	14.991379	30
CGTCGTA	585	0.0	14.863247	10
ACGACGG	760	0.0	14.848682	6
TACCGAC	140	6.003738E-7	14.535714	7
>>END_MODULE