##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727212.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4314374
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310324047011225	31.0	31.0	34.0	30.0	34.0
2	31.52409040106398	31.0	31.0	34.0	30.0	34.0
3	31.6774373292626	31.0	31.0	34.0	30.0	34.0
4	35.47863861593826	37.0	35.0	37.0	33.0	37.0
5	35.325120631637404	37.0	35.0	37.0	33.0	37.0
6	35.35076861672169	37.0	35.0	37.0	33.0	37.0
7	35.24725974150595	37.0	35.0	37.0	32.0	37.0
8	35.275346319072014	37.0	35.0	37.0	32.0	37.0
9	36.844300702720716	39.0	37.0	39.0	33.0	39.0
10	36.57947873781921	39.0	35.0	39.0	32.0	39.0
11	36.750095610626246	39.0	35.0	39.0	32.0	39.0
12	36.62326261005652	39.0	35.0	39.0	32.0	39.0
13	36.682985990551586	39.0	35.0	39.0	32.0	39.0
14	37.693288528069196	39.0	37.0	41.0	32.0	41.0
15	37.687896552315586	39.0	37.0	41.0	32.0	41.0
16	37.6361738690248	39.0	36.0	41.0	32.0	41.0
17	37.659049493622945	39.0	36.0	41.0	32.0	41.0
18	37.61248097638267	39.0	36.0	41.0	32.0	41.0
19	37.637709665411485	39.0	37.0	41.0	32.0	41.0
20	37.58404417419538	39.0	36.0	41.0	32.0	41.0
21	37.4900309987034	39.0	36.0	41.0	32.0	41.0
22	37.42038497357902	39.0	36.0	41.0	32.0	41.0
23	37.30498468607497	39.0	36.0	41.0	31.0	41.0
24	37.16725439194655	39.0	36.0	41.0	31.0	41.0
25	37.05123617006778	39.0	36.0	40.0	31.0	41.0
26	36.81679729202892	39.0	35.0	40.0	30.0	41.0
27	36.6481633256644	39.0	35.0	40.0	30.0	41.0
28	36.42046656131341	38.0	35.0	40.0	30.0	41.0
29	36.334477724926025	38.0	35.0	40.0	30.0	41.0
30	36.2019588473322	38.0	35.0	40.0	30.0	41.0
31	36.27737604574847	38.0	35.0	40.0	30.0	41.0
32	36.19872222482335	38.0	35.0	40.0	30.0	41.0
33	36.13307956148447	38.0	35.0	40.0	30.0	41.0
34	36.141310419541746	38.0	35.0	40.0	30.0	41.0
35	35.90073461410624	38.0	35.0	40.0	29.0	41.0
36	35.82916061519006	38.0	35.0	40.0	28.0	41.0
37	35.70683695942911	38.0	34.0	40.0	27.0	41.0
38	35.57069716255475	38.0	34.0	40.0	27.0	41.0
39	35.458328601090216	38.0	34.0	40.0	27.0	41.0
40	35.203386864467475	38.0	34.0	40.0	26.0	41.0
41	35.07719659908946	38.0	34.0	40.0	25.0	41.0
42	34.80851335558762	38.0	33.0	40.0	24.0	41.0
43	34.408701470943406	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	9.0
15	28.0
16	99.0
17	203.0
18	334.0
19	663.0
20	1410.0
21	2742.0
22	5031.0
23	8558.0
24	14437.0
25	22118.0
26	33595.0
27	49037.0
28	68691.0
29	92613.0
30	119684.0
31	152113.0
32	188378.0
33	233729.0
34	293397.0
35	362062.0
36	456423.0
37	607414.0
38	777700.0
39	823900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75163117522959	19.129495959321098	13.013498597942599	24.10537426750671
2	17.548617713716986	23.98456879259888	34.01394964831515	24.45286384536899
3	20.37210960384983	25.953313273258182	29.881438187788078	23.79313893510391
4	14.44561829827456	15.923677455871928	33.849406657837264	35.781297588016244
5	13.933632086601671	38.875025670004504	32.549843847566294	14.64149839582753
6	33.42679610066258	34.73414683103505	16.49972858171313	15.339328486589249
7	27.526751273765328	32.233366880108214	21.851003181458076	18.388878664668383
8	28.739534402905264	32.570797061172726	20.54022205770756	18.149446478214454
9	28.38968063501217	13.23656688084992	20.015279157532472	38.358473326605434
10	17.469672309354728	27.497407503382874	31.570350646466906	23.46256954079549
11	35.112625840967894	23.27540449668944	20.656670005891932	20.955299656450737
12	21.48726559171736	26.9962223951841	29.280887563294232	22.235624449804305
13	30.06542780018607	19.581913853550944	25.62888613736315	24.72377220889983
14	23.01963158502253	21.488679470069123	26.193973911394792	29.297715033513555
15	25.63674359246556	27.611027694863726	23.507720934717298	23.24450777795342
16	23.381144054734243	26.29092888099177	26.247631753760803	24.080295310513183
17	23.26045447149459	26.899313782254392	26.429257176127983	23.410974570123035
18	23.051524972104875	24.507402464413143	28.784917580163427	23.656154983318554
19	23.105669559477228	26.507692657150262	28.794791550292114	21.591846233080396
20	23.04299534532704	25.173988161434313	28.4867978529446	23.29621864029405
21	23.96500627901058	26.19172561303216	28.116523973118696	21.726744134838565
22	24.029534759851604	25.652273076001293	27.12792168690058	23.190270477246525
23	22.145599801964316	26.1057108169111	28.022535830227053	23.726153550897536
24	22.1675728622507	27.310798739284074	27.820188977589794	22.701439420875428
25	22.926408327140855	25.88540075570639	27.156106540601254	24.0320843765515
26	22.457000714356244	26.984610050032753	28.13571563336883	22.42267360224218
27	23.611977079409435	26.561559104518988	26.98776230340717	22.83870151266441
28	22.11838843827633	27.34922841645161	28.206247302621424	22.326135842650636
29	22.267193340215755	27.206310811255584	28.437057148962978	22.089438699565683
30	22.26874628856933	27.88719754012981	28.168049408790246	21.67600676251062
31	22.64316909011597	27.381631726873934	27.170801604126115	22.804397578883982
32	21.53950955573161	27.62171290666966	27.43049165417741	23.408285883421325
33	21.418333227485608	27.15399731224043	28.051601460605873	23.376067999668088
34	22.3770818199813	27.211641827991734	28.224001906186157	22.18727444584081
35	22.370406459894298	27.159954143984738	28.524091791763993	21.94554760435697
36	21.508867798665577	27.54603564735	28.05760464901745	22.88749190496698
37	21.68421189261756	27.27475643048099	28.360429577964265	22.68060209893718
38	21.527062790569385	26.539516509231696	28.751795741398407	23.181624958800512
39	21.754025033527462	26.03109976093867	28.733554392827326	23.48132081270655
40	21.729989101547524	26.448726976381742	29.281698804971473	22.539585117099257
41	20.533199022616028	26.82901389633815	29.775629094742367	22.86215798630346
42	21.422806645877245	26.92297886089616	29.42215950680215	22.232054986424448
43	20.504481067241738	25.861388002060092	29.479525882549822	24.154605048148355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2122.0
1	2170.5
2	2219.0
3	4758.0
4	7297.0
5	7297.0
6	11008.0
7	14719.0
8	13954.5
9	13190.0
10	17392.0
11	21594.0
12	21594.0
13	36825.5
14	52057.0
15	79122.0
16	106187.0
17	92835.0
18	79483.0
19	79483.0
20	80653.0
21	81823.0
22	63361.0
23	44899.0
24	50283.0
25	55667.0
26	55667.0
27	61536.5
28	67406.0
29	73162.0
30	78918.0
31	84080.0
32	89242.0
33	89242.0
34	100900.5
35	112559.0
36	131856.5
37	151154.0
38	171672.0
39	192190.0
40	192190.0
41	211988.0
42	231786.0
43	244583.5
44	257381.0
45	285114.5
46	312848.0
47	312848.0
48	351693.5
49	390539.0
50	384777.0
51	379015.0
52	346594.5
53	314174.0
54	314174.0
55	300840.0
56	287506.0
57	275189.0
58	262872.0
59	243107.5
60	223343.0
61	223343.0
62	187910.5
63	152478.0
64	134615.0
65	116752.0
66	98251.0
67	79750.0
68	79750.0
69	65939.5
70	52129.0
71	44089.0
72	36049.0
73	26433.0
74	16817.0
75	16817.0
76	13244.5
77	9672.0
78	8529.5
79	7387.0
80	5998.0
81	4609.0
82	4609.0
83	3798.0
84	2987.0
85	2628.5
86	2270.0
87	1455.5
88	641.0
89	641.0
90	488.5
91	336.0
92	239.5
93	143.0
94	147.5
95	152.0
96	152.0
97	80.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4314374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.94965210313295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30709091397131	35.73418359186841
2	9.41682708756933	8.277325488280614
3	3.137840702671397	4.1372102171237435
4	1.5709611785375346	2.761727890570095
5	0.9763146746847908	2.145434514778999
6	0.6553203020264439	1.728065957410933
7	0.43486975003335027	1.3378661956899491
8	0.35034484147212747	1.2318027119061812
9	0.266058411193076	1.0523857149943185
>10	1.6469856431091583	13.45944737190911
>50	0.12277947179310908	3.7304596839034727
>100	0.0891885610391327	8.046727933491587
>500	0.014752259755928268	4.535604831573132
>1k	0.010188611002448516	8.045010264881212
>5k	2.6532841152209673E-4	0.7648976279451548
>10k+	2.1226272921767738E-4	3.0118500036730564
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48492	1.1239637546489942	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32719	0.7583718982174471	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30064	0.6968334224153956	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17866	0.4141041087304902	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	8930	0.2069825193643388	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6225	0.14428512688051615	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	6074	0.1407851985015671	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5986	0.13874550514164974	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5582	0.12938145835293832	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4784	0.11088514811186977	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4635	0.10743157640019155	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	4584	0.1062494813847849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.635666727084856E-5
2	0.0	0.0	0.0	0.0	4.635666727084856E-5
3	0.0	0.0	0.0	0.0	4.635666727084856E-5
4	0.0	0.0	0.0	0.0	4.635666727084856E-5
5	0.0	0.0	0.0	0.0	4.635666727084856E-5
6	0.0	0.0	0.0	0.0	6.953500090627285E-5
7	0.0	0.0	0.0	0.0	6.953500090627285E-5
8	0.0	0.0	0.0	0.0	1.1589166817712141E-4
9	0.0	0.0	0.0	0.0	1.1589166817712141E-4
10	0.0	0.0	0.0	0.0	1.1589166817712141E-4
11	0.0	0.0	0.0	4.635666727084856E-5	1.1589166817712141E-4
12	0.0	0.0	0.0	1.8542666908339425E-4	1.1589166817712141E-4
13	0.0	0.0	0.0	3.4767500453136425E-4	1.1589166817712141E-4
14	0.0	0.0	0.0	6.026366745210313E-4	1.1589166817712141E-4
15	0.0	0.0	0.0	0.0011357383481357898	1.8542666908339425E-4
16	0.0	0.0	0.0	0.0029436483716988836	2.0860500271881852E-4
17	0.0	0.0	0.0	0.004311170056188917	2.0860500271881852E-4
18	0.0	0.0	0.0	0.004890628397074524	2.0860500271881852E-4
19	0.0	0.0	0.0	0.006629003419731345	2.0860500271881852E-4
20	0.0	0.0	0.0	0.00834420010875274	2.0860500271881852E-4
21	0.0	0.0	0.0	0.011936841822243504	2.549616699896671E-4
22	0.0	0.0	0.0	0.019006233581047912	2.781400036250914E-4
23	0.0	0.0	0.0	0.029204700380634593	3.708533381667885E-4
24	0.0	0.0	0.0	0.0428567388918995	3.708533381667885E-4
25	0.0	0.0	0.0	0.04807186395986996	4.1721000543763705E-4
26	0.0	0.0	0.0	0.05884978910034225	4.1721000543763705E-4
27	0.0	0.0	0.0	0.10140520965498123	4.1721000543763705E-4
28	0.0	0.0	0.0	0.18062875402086143	4.1721000543763705E-4
29	0.0	0.0	0.0	0.281779002005853	4.1721000543763705E-4
30	0.0	0.0	0.0	0.4437723757838333	4.1721000543763705E-4
31	0.0	0.0	0.0	0.8848792431995928	4.1721000543763705E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1485	0.0	18.686869	5
GTACTAG	100	2.8794784E-7	18.499998	1
CAAGACG	1565	0.0	17.84984	4
GACGGAC	1730	0.0	16.040462	7
CTTATAC	4385	0.0	15.989738	37
ACGGACC	1715	0.0	15.965014	8
AGACGGA	1820	0.0	15.552198	6
GCGCAAG	1740	0.0	15.310346	1
CGCAAGA	1750	0.0	15.117143	2
CGGACCA	1790	0.0	14.9860325	9
ACCGTCG	1185	0.0	14.675106	8
ATACCGT	1290	0.0	14.627906	6
TCTAGCG	805	0.0	14.478261	28
AGAGCGA	1880	0.0	14.268618	15
GCGAAAG	1920	0.0	14.260416	18
CCGTCGT	1195	0.0	14.242678	9
TACCGTC	1225	0.0	14.195918	7
CTAGCGG	835	0.0	14.179641	29
TATACCG	210	1.364242E-10	14.095239	5
AGCGAAA	1970	0.0	13.992385	17
>>END_MODULE