FastQCFastQC Report
Thu 9 Feb 2017
SRR2727210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260493
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT304470.9338158370528628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT196370.6022708835749685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT189890.5823965884913723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122970.37715155346139373No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA48680.1493025747946706No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41370.12688265240870014No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39630.12154603613625302No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG36860.11305038839218487No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC35050.10749908066050133No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32890.10087431563263594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG603.7289887E-521.58333418
AAGACGG10600.018.8490565
CAAGACG10400.018.3221154
CGAGCCG8650.017.323715
CTTATAC36950.016.42219237
CGTTATA700.002593863515.8571422
AGACGGA12700.015.2952766
GACGGAC12400.015.2177427
ACGGACC12300.015.040658
TATACCG1601.0986696E-815.0312515
GCGCAAG13050.014.8850581
AGGCCCG10700.014.69626110
CGGACCA13100.014.2633599
GCCGCCT10850.014.15207418
CGCAAGA14100.014.0390072
ATACCGC10600.013.78773627
TACCGCA10900.013.74770628
GCAAGAC15450.013.5307443
ATACCGT9600.013.4895846
GGAGTGT9200.013.4728266
Produced by FastQC (version 0.11.5)