FastQCFastQC Report
Thu 9 Feb 2017
SRR2727209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150586
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121481.0558098221254213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83610.7266731908783872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76280.6629665231455971No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.4148320942545799No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA16670.14488269455738206No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.14436122115165664No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT13920.12098183012829984No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.10533762795653694No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.10490306678509907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCG603.72629E-521.5833347
CTATACT1205.173206E-918.54
GACCGTG1205.173206E-918.57
TCTATAC953.6063939E-617.5263163
TTAGTAC852.723119E-517.4117643
AGACCGT1107.807612E-716.8181826
CTTATAC11100.016.537
TGTGCGA904.445963E-516.44444510
AGGTCCG2900.015.948276529
CTAGACA700.002592495615.8571434
CGTGCGC700.002592495615.85714310
ACCGACT700.002592495615.85714313
ATACAGT1302.5887675E-715.6538466
GTATTAG1701.4861143E-915.2352941
TATACTC855.364156E-415.2352945
AGGCGTG1101.4517918E-515.1363647
TCGTTAC2950.015.05084723
ATCGCTC2950.015.05084716
GGTCCGA1601.096123E-815.03125130
TACCGTC2600.014.9423077
Produced by FastQC (version 0.11.5)