##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727209.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150586
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290874389224275	31.0	31.0	34.0	30.0	34.0
2	31.524331079988805	31.0	31.0	34.0	30.0	34.0
3	31.671875896282415	31.0	31.0	34.0	30.0	34.0
4	35.472434915773356	37.0	35.0	37.0	33.0	37.0
5	35.31854289900972	37.0	35.0	37.0	33.0	37.0
6	35.337930411112254	37.0	35.0	37.0	33.0	37.0
7	35.243028335126624	37.0	35.0	37.0	32.0	37.0
8	35.26422970555873	37.0	35.0	37.0	32.0	37.0
9	36.82859777539445	39.0	37.0	39.0	33.0	39.0
10	36.55512669196392	39.0	35.0	39.0	32.0	39.0
11	36.733674840472595	39.0	35.0	39.0	32.0	39.0
12	36.60170730393035	39.0	35.0	39.0	32.0	39.0
13	36.667172206162775	39.0	35.0	39.0	32.0	39.0
14	37.668612341884916	39.0	37.0	41.0	32.0	41.0
15	37.671433513009895	39.0	36.0	41.0	32.0	41.0
16	37.611486668532386	39.0	36.0	41.0	32.0	41.0
17	37.63160337428059	39.0	36.0	41.0	32.0	41.0
18	37.58506448018662	39.0	36.0	41.0	32.0	41.0
19	37.61764005471994	39.0	36.0	41.0	32.0	41.0
20	37.552748773233816	39.0	36.0	41.0	32.0	41.0
21	37.462313986090564	39.0	36.0	41.0	32.0	41.0
22	37.38208964823142	39.0	36.0	41.0	32.0	41.0
23	37.269281044615525	39.0	36.0	41.0	31.0	41.0
24	37.13159989779121	39.0	36.0	41.0	31.0	41.0
25	37.008215813507206	39.0	36.0	40.0	31.0	41.0
26	36.78014941951319	39.0	35.0	40.0	30.0	41.0
27	36.61267736614212	39.0	35.0	40.0	30.0	41.0
28	36.38216091626354	38.0	35.0	40.0	30.0	41.0
29	36.29257873813865	38.0	35.0	40.0	30.0	41.0
30	36.18369856751256	38.0	35.0	40.0	30.0	41.0
31	36.25724022367733	38.0	35.0	40.0	30.0	41.0
32	36.20488603198718	38.0	35.0	40.0	30.0	41.0
33	36.14311055410026	38.0	35.0	40.0	30.0	41.0
34	36.164780381475175	38.0	35.0	40.0	30.0	41.0
35	35.939712459564085	38.0	35.0	40.0	29.0	41.0
36	35.872322451342185	38.0	35.0	40.0	28.0	41.0
37	35.77764547804336	38.0	35.0	40.0	28.0	41.0
38	35.66952057473322	38.0	34.0	40.0	27.0	41.0
39	35.56449930730949	38.0	34.0	40.0	27.0	41.0
40	35.336910930604056	38.0	34.0	40.0	26.0	41.0
41	35.22636204507964	38.0	34.0	40.0	26.0	41.0
42	34.986063623231985	38.0	33.0	40.0	25.0	41.0
43	34.610935644967	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	2.0
16	24.0
17	40.0
18	68.0
19	168.0
20	352.0
21	717.0
22	1276.0
23	2277.0
24	3772.0
25	5973.0
26	8690.0
27	12732.0
28	18111.0
29	24565.0
30	32032.0
31	40514.0
32	50160.0
33	62788.0
34	78159.0
35	97260.0
36	122367.0
37	160761.0
38	206949.0
39	220825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88692892143656	19.471903882021856	13.474351330539394	24.166815866002196
2	17.407825229926317	23.496114154005003	35.00668355081672	24.08937706525197
3	20.0233620085765	25.378633148673806	30.690274347158752	23.907730495590943
4	14.250564494961699	16.080327763417944	34.33763317127099	35.33147457034937
5	13.506595769460084	39.275551762319374	33.00196595473959	14.215886513480957
6	33.00387802389391	35.28601947181697	16.5387028870506	15.17139961723852
7	27.200921964981323	33.08288124486131	21.628457151399374	18.087739638757988
8	28.362243239531853	33.423490291034305	20.493991757243702	17.720274712190136
9	28.477923423368612	13.598548913336334	19.996419215947352	37.9271084473477
10	17.15751799517811	27.95949194584325	31.866023052601022	23.01696700637762
11	35.20831993436388	23.386430914334085	20.72674272066582	20.678506430636215
12	21.117326301554165	26.984597413839555	29.61551765795864	22.28255862664764
13	29.904500836964814	20.062211777303045	25.791031700368332	24.242255685363805
14	22.581623624831174	21.74891750812195	26.53899838864718	29.1304604783997
15	25.536552678374324	28.150003563401604	23.628481486824974	22.684962271399094
16	23.049993655406897	26.952874448324593	26.351181050351734	23.645950845916776
17	22.821762128167734	27.20639743574144	26.413931683507357	23.55790875258347
18	22.552334201876263	25.48953315962475	28.760214360334647	23.197918278164344
19	23.004625469108785	26.69274613110189	28.968890634859108	21.33373776493022
20	22.78977842594991	25.131194017657087	28.74213661560283	23.336890940790173
21	23.73268925573577	26.39611467547841	28.632018814760478	21.23917725402534
22	24.164643060145004	25.483188566521754	27.37040082184209	22.981767551491153
23	22.155579852353497	26.375255739249393	28.24612849452366	23.223035913873453
24	21.821663048220646	26.977122961690824	28.43142537802476	22.769788612063767
25	22.893290896986404	25.758005051339055	27.49529370251333	23.853410349161212
26	22.081965189911923	26.942705716912947	28.524421468712465	22.450907624462666
27	23.691058295512025	26.631125356992	27.352670726047425	22.32514562144855
28	21.96776251405805	27.69249756211183	27.98556561612952	22.354174307700596
29	22.111602261803988	27.36353475533337	28.696942253773294	21.82792072908935
30	22.75466588329773	27.591940107041108	28.028760996570444	21.624633013090722
31	22.425007778644968	27.33346312226987	27.78905705440532	22.45247204467984
32	21.766213042745175	27.536837750502784	27.60115280387559	23.09579640287645
33	21.280895126483372	26.857184078373976	28.391011189081045	23.470909606061607
34	22.376250015209642	27.215783957044497	28.765168357689035	21.642797670056822
35	22.163401953439376	26.841887525139363	28.870071424474137	22.12463909694712
36	21.221707894933537	27.33737417281281	28.689120152687416	22.75179777956624
37	21.493134802613625	26.986335658525306	29.0416361749578	22.478893363903264
38	21.61064014337042	26.10721840870652	28.946988751818637	23.33515269610442
39	21.348860493696257	26.07897193256306	29.464464194766844	23.107703378973845
40	21.815579191820518	26.02456487389904	29.688176285823047	22.471679648457396
41	20.456967145437194	26.563594550950558	30.38338724788934	22.59605105572291
42	21.020332248089236	26.54273561472154	30.04573321768212	22.3911989195071
43	20.057779253354376	25.564190768877772	29.90754276516488	24.470487212602972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1742.0
1	1474.5
2	1207.0
3	1808.0
4	2409.0
5	2409.0
6	3320.5
7	4232.0
8	3948.0
9	3664.0
10	4787.5
11	5911.0
12	5911.0
13	9873.0
14	13835.0
15	19964.0
16	26093.0
17	22972.5
18	19852.0
19	19852.0
20	20091.5
21	20331.0
22	16470.0
23	12609.0
24	14255.5
25	15902.0
26	15902.0
27	17591.5
28	19281.0
29	21125.0
30	22969.0
31	24748.5
32	26528.0
33	26528.0
34	29324.0
35	32120.0
36	36805.5
37	41491.0
38	46082.0
39	50673.0
40	50673.0
41	56294.0
42	61915.0
43	65440.0
44	68965.0
45	76700.0
46	84435.0
47	84435.0
48	93853.5
49	103272.0
50	102377.5
51	101483.0
52	95819.0
53	90155.0
54	90155.0
55	84635.0
56	79115.0
57	74051.0
58	68987.0
59	62766.5
60	56546.0
61	56546.0
62	47365.0
63	38184.0
64	34024.0
65	29864.0
66	24617.0
67	19370.0
68	19370.0
69	15527.0
70	11684.0
71	9563.5
72	7443.0
73	5315.5
74	3188.0
75	3188.0
76	2495.0
77	1802.0
78	1593.0
79	1384.0
80	1077.5
81	771.0
82	771.0
83	655.5
84	540.0
85	466.5
86	393.0
87	265.5
88	138.0
89	138.0
90	93.0
91	48.0
92	39.0
93	30.0
94	26.5
95	23.0
96	23.0
97	12.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1150586.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.312769551647705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.43784656588308	48.96679611219492
2	7.686481286334579	8.810670612534926
3	2.49893319645966	4.296623472409646
4	1.243735809786109	2.8512777539761287
5	0.7583293502234945	2.173097764680494
6	0.4844765970558102	1.6660017336135682
7	0.3289298079632002	1.3196314797723838
8	0.23443168990752453	1.0748743535418632
9	0.17739678482541532	0.9150390943112036
>10	0.9859068200139123	10.234956109761303
>50	0.08430183213396192	3.3878809030744064
>100	0.07113930985516284	8.05271355049789
>500	0.006564355301812822	2.6513108276712645
>1k	0.001068615979364878	1.1373259891396486
>5k	3.05318851247108E-4	1.398931090111073
>10k+	1.52659425623554E-4	1.06286915270932
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12148	1.0558098221254213	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8361	0.7266731908783872	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7628	0.6629665231455971	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4773	0.4148320942545799	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1667	0.14488269455738206	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1661	0.14436122115165664	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1392	0.12098183012829984	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.10533762795653694	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.10490306678509907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	8.691223428757172E-5	0.0
13	0.0	0.0	0.0	8.691223428757172E-5	0.0
14	0.0	8.691223428757172E-5	0.0	2.6073670286271515E-4	0.0
15	0.0	8.691223428757172E-5	0.0	8.691223428757172E-4	8.691223428757172E-5
16	0.0	8.691223428757172E-5	0.0	0.0019989813886141495	8.691223428757172E-5
17	0.0	8.691223428757172E-5	0.0	0.0029550159657774386	8.691223428757172E-5
18	0.0	8.691223428757172E-5	0.0	0.003737226074365584	8.691223428757172E-5
19	0.0	8.691223428757172E-5	0.0	0.004606348417241301	8.691223428757172E-5
20	0.0	8.691223428757172E-5	0.0	0.00556238299440459	8.691223428757172E-5
21	0.0	8.691223428757172E-5	0.0	0.00756136438301874	8.691223428757172E-5
22	0.0	8.691223428757172E-5	0.0	0.011820063863109754	8.691223428757172E-5
23	0.0	8.691223428757172E-5	0.0	0.017817008028952204	1.7382446857514344E-4
24	0.0	8.691223428757172E-5	0.0	0.027464266034872665	1.7382446857514344E-4
25	0.0	8.691223428757172E-5	0.0	0.031549141046388535	1.7382446857514344E-4
26	0.0	8.691223428757172E-5	0.0	0.03911050542940728	1.7382446857514344E-4
27	0.0	8.691223428757172E-5	0.0	0.07248480339583482	1.7382446857514344E-4
28	0.0	8.691223428757172E-5	0.0	0.14644711477455835	1.7382446857514344E-4
29	0.0	8.691223428757172E-5	0.0	0.2519585671996704	1.7382446857514344E-4
30	0.0	8.691223428757172E-5	0.0	0.41213781499166513	1.7382446857514344E-4
31	0.0	8.691223428757172E-5	0.0	0.8785088641787749	1.7382446857514344E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGACCG	60	3.72629E-5	21.583334	7
CTATACT	120	5.173206E-9	18.5	4
GACCGTG	120	5.173206E-9	18.5	7
TCTATAC	95	3.6063939E-6	17.526316	3
TTAGTAC	85	2.723119E-5	17.411764	3
AGACCGT	110	7.807612E-7	16.818182	6
CTTATAC	1110	0.0	16.5	37
TGTGCGA	90	4.445963E-5	16.444445	10
AGGTCCG	290	0.0	15.9482765	29
CTAGACA	70	0.0025924956	15.857143	4
CGTGCGC	70	0.0025924956	15.857143	10
ACCGACT	70	0.0025924956	15.857143	13
ATACAGT	130	2.5887675E-7	15.653846	6
GTATTAG	170	1.4861143E-9	15.235294	1
TATACTC	85	5.364156E-4	15.235294	5
AGGCGTG	110	1.4517918E-5	15.136364	7
TCGTTAC	295	0.0	15.050847	23
ATCGCTC	295	0.0	15.050847	16
GGTCCGA	160	1.096123E-8	15.031251	30
TACCGTC	260	0.0	14.942307	7
>>END_MODULE