FastQCFastQC Report
Thu 9 Feb 2017
SRR2727208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4968276
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT407410.8200228811764887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT281300.5661923773961028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT264470.5323174477424362No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148430.29875554417669226No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG74190.1493274528226693No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA72210.14534216698106145No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70770.14244377727807395No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT67020.13489588742654393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15900.019.4308195
CAAGACG17100.018.3918134
CTTATAC39400.016.66878337
ACGGACC18650.015.7721188
AGACGGA19250.015.5688316
GACGGAC18950.015.5224287
CGGACCA19450.015.2185099
GTACCGT2500.014.7999996
CGAGCCG14950.014.72575215
CGAACGA10850.014.3225816
CTAGCGG10600.014.31132129
GCGCAAG21800.014.2568811
CGCAAGA21350.014.21077252
TACCGTC12850.013.964987
GCAAGAC24000.013.9520843
TCTAGCG10500.013.91904728
AGAGCGA21300.013.80985915
TCTTATA63350.013.75453837
ACGAACG11300.013.75221315
TATACTG7400.013.7500015
Produced by FastQC (version 0.11.5)