##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4968276
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286812769660944	31.0	31.0	34.0	30.0	34.0
2	31.521560798957225	31.0	31.0	34.0	30.0	34.0
3	31.67673051980204	31.0	31.0	34.0	30.0	34.0
4	35.48116811545896	37.0	35.0	37.0	33.0	37.0
5	35.32035217045108	37.0	35.0	37.0	33.0	37.0
6	35.3390761704865	37.0	35.0	37.0	33.0	37.0
7	35.24223956157025	37.0	35.0	37.0	32.0	37.0
8	35.258640019193784	37.0	35.0	37.0	32.0	37.0
9	36.836619382659094	39.0	37.0	39.0	33.0	39.0
10	36.56372290911374	39.0	35.0	39.0	32.0	39.0
11	36.73236591525914	39.0	35.0	39.0	32.0	39.0
12	36.598084929259166	39.0	35.0	39.0	32.0	39.0
13	36.65670164056908	39.0	35.0	39.0	32.0	39.0
14	37.680845025517904	39.0	37.0	41.0	32.0	41.0
15	37.67727457170254	39.0	37.0	41.0	32.0	41.0
16	37.624587080105854	39.0	36.0	41.0	32.0	41.0
17	37.62720569469168	39.0	36.0	41.0	32.0	41.0
18	37.566743473993796	39.0	36.0	41.0	32.0	41.0
19	37.58295412734719	39.0	36.0	41.0	32.0	41.0
20	37.52118843639121	39.0	36.0	41.0	32.0	41.0
21	37.42476585439295	39.0	36.0	41.0	32.0	41.0
22	37.35569622138544	39.0	36.0	41.0	31.0	41.0
23	37.24428554291267	39.0	36.0	41.0	31.0	41.0
24	37.10311886859748	39.0	36.0	40.0	31.0	41.0
25	36.98601084158771	39.0	36.0	40.0	31.0	41.0
26	36.760690026077455	39.0	35.0	40.0	30.0	41.0
27	36.5998032315435	39.0	35.0	40.0	30.0	41.0
28	36.36937098502579	38.0	35.0	40.0	30.0	41.0
29	36.296047562575026	38.0	35.0	40.0	30.0	41.0
30	36.19159603854536	38.0	35.0	40.0	30.0	41.0
31	36.30200475979998	38.0	35.0	40.0	30.0	41.0
32	36.25082422957179	38.0	35.0	40.0	30.0	41.0
33	36.21188074092502	38.0	35.0	40.0	30.0	41.0
34	36.23855256833558	38.0	35.0	40.0	30.0	41.0
35	36.02410554486104	38.0	35.0	40.0	29.0	41.0
36	35.97407148878202	38.0	35.0	40.0	29.0	41.0
37	35.879527023055886	38.0	35.0	40.0	28.0	41.0
38	35.76760570467502	38.0	34.0	40.0	28.0	41.0
39	35.67874751724743	38.0	34.0	40.0	27.0	41.0
40	35.45895819797451	38.0	34.0	40.0	26.0	41.0
41	35.3699856851753	38.0	34.0	40.0	26.0	41.0
42	35.1358503432579	38.0	34.0	40.0	25.0	41.0
43	34.75378763176603	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	12.0
15	31.0
16	95.0
17	213.0
18	361.0
19	730.0
20	1500.0
21	2964.0
22	5571.0
23	9822.0
24	16002.0
25	24919.0
26	37966.0
27	54841.0
28	77062.0
29	104106.0
30	135619.0
31	172492.0
32	215872.0
33	269297.0
34	336661.0
35	415602.0
36	525970.0
37	690816.0
38	910772.0
39	958972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99692690180658	19.263563457424667	12.894211191165708	24.845298449603042
2	17.69034167989057	23.23184138723372	34.300368981111355	24.77744795176435
3	20.106411157512184	25.153010823070215	30.019829816217943	24.720748203199662
4	14.491062895861665	15.918902251002159	34.2101364738996	35.37989837923658
5	14.004274319703654	38.39102336504654	33.129761712111	14.474940603138794
6	33.764227269177475	35.01546612949844	16.25350524004705	14.96680136127703
7	27.959457163812964	32.05027256939832	21.601295902240537	18.388974364548186
8	28.262298632362615	33.14719230574147	20.42410687328965	18.16640218860627
9	28.25581751094343	13.417471171086309	19.612879799753475	38.71383151821678
10	17.273355989079512	27.138709685210728	31.998202193275898	23.589732132433866
11	35.78110394833137	22.788005336257484	20.69800470022197	20.732886015189173
12	21.540953038840836	26.403263425783912	29.27095837670854	22.784825158666706
13	30.08256787666386	19.54106414377945	25.360285942246364	25.016082037310326
14	22.92310250074674	21.264861291925005	25.63678024328761	30.17525596404064
15	25.742068274789887	27.63614581798596	22.89806363414593	23.723722273078227
16	23.92149711489458	26.193572981855272	25.656525523139212	24.228404380110927
17	23.611007117962046	26.658019804052753	25.90121804827268	23.829755029712523
18	23.552012810882488	24.610267223479532	27.95917537592517	23.87854458971281
19	23.669296955322128	26.27893458414951	27.8356516425416	22.216116817986762
20	23.461659537433103	24.940804415857734	27.60353893382735	23.99399711288181
21	24.364709206976425	26.008699999758466	27.350594049122872	22.275996744142233
22	24.537002372654015	25.322486109869903	26.33406437162509	23.806447145850996
23	22.67993968128985	25.71195722620885	27.27169344054155	24.33640965195975
24	22.73239248383141	26.80241596883909	27.08355977002888	23.38163177730062
25	23.612798483820143	25.391705291735	26.37077730786293	24.624718916581926
26	22.990610827578823	26.52779354448102	27.388091965905275	23.093503662034877
27	24.341340939996087	25.996321460401962	26.270179031921735	23.39215856768022
28	22.71854462191714	26.806340871561886	27.38076548082272	23.09434902569825
29	22.903035177594806	26.65711405727057	27.660842513580164	22.779008251554465
30	23.079212990582647	27.190478145739082	27.353532694238403	22.376776169439864
31	23.369957707663584	26.71455853096728	26.46225773286347	23.453226028505664
32	22.376816424852404	26.9315553322722	26.665728715554447	24.025899527320945
33	22.143677203118344	26.39557464198849	27.33668580408979	24.124062350803378
34	23.148915237398242	26.5812728600424	27.549375276252768	22.72043662630659
35	23.14476892990647	26.475562146708437	27.842032125429423	22.537636797955667
36	22.209213014735894	26.903497309730778	27.38223480338049	23.505054872152837
37	22.549572527774224	26.564748013194112	27.762326408597264	23.123353050434396
38	22.339439274307626	25.840955695698064	27.979242699077105	23.840362330917202
39	22.41439485245989	25.44423860510165	28.111119430563036	24.03024711187543
40	22.512960230067733	25.769582849261997	28.602577634575855	23.11487928609441
41	21.27029577261811	26.12169694276244	29.167240306295383	23.44076697832407
42	22.260055600775804	26.15255271647549	28.823459083191032	22.763932599557673
43	21.32667347788247	25.059557882855138	28.897368020617215	24.716400618645178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2225.0
1	2148.0
2	2071.0
3	4025.5
4	5980.0
5	5980.0
6	9021.0
7	12062.0
8	11469.0
9	10876.0
10	14484.0
11	18092.0
12	18092.0
13	30965.5
14	43839.0
15	66059.5
16	88280.0
17	79171.5
18	70063.0
19	70063.0
20	72554.5
21	75046.0
22	60731.5
23	46417.0
24	52740.0
25	59063.0
26	59063.0
27	66401.5
28	73740.0
29	81146.0
30	88552.0
31	96573.5
32	104595.0
33	104595.0
34	118122.5
35	131650.0
36	154072.5
37	176495.0
38	199945.0
39	223395.0
40	223395.0
41	246395.5
42	269396.0
43	285302.5
44	301209.0
45	332577.5
46	363946.0
47	363946.0
48	413462.0
49	462978.0
50	460146.0
51	457314.0
52	420601.5
53	383889.0
54	383889.0
55	366601.5
56	349314.0
57	332729.0
58	316144.0
59	292552.0
60	268960.0
61	268960.0
62	226186.5
63	183413.0
64	161224.0
65	139035.0
66	116822.5
67	94610.0
68	94610.0
69	77152.5
70	59695.0
71	49657.5
72	39620.0
73	28795.0
74	17970.0
75	17970.0
76	13811.0
77	9652.0
78	8662.0
79	7672.0
80	6064.5
81	4457.0
82	4457.0
83	3724.5
84	2992.0
85	2618.5
86	2245.0
87	1466.5
88	688.0
89	688.0
90	501.0
91	314.0
92	234.5
93	155.0
94	154.5
95	154.0
96	154.0
97	79.5
98	5.0
99	6.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4968276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.29872203839911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1200592363085	35.935149558477015
2	9.429259825203665	8.354083200490821
3	3.217952582964925	4.27653561016535
4	1.5935273850748592	2.823649067680327
5	0.9527236668708351	2.110222044905774
6	0.6298552720661598	1.6741070173007437
7	0.4573061973501714	1.4180656085996775
8	0.3478313144177869	1.2326786170915638
9	0.2749014950901826	1.0960006427046303
>10	1.7270304417366804	14.306171512195931
>50	0.13813328706703193	4.170709354494538
>100	0.08831205756075194	7.705400600903893
>500	0.01287794747330183	4.039196885485455
>1k	0.00986395900032734	8.054603214281206
>5k	1.826659074134652E-4	0.5749068679937481
>10k+	1.826659074134652E-4	2.228520197229293
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40741	0.8200228811764887	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28130	0.5661923773961028	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26447	0.5323174477424362	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14843	0.29875554417669226	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	7419	0.1493274528226693	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	7221	0.14534216698106145	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	7077	0.14244377727807395	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6702	0.13489588742654393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.0127706270746635E-5
7	0.0	0.0	0.0	0.0	2.0127706270746635E-5
8	0.0	0.0	0.0	0.0	2.0127706270746635E-5
9	0.0	0.0	0.0	2.0127706270746635E-5	8.051082508298654E-5
10	0.0	2.0127706270746635E-5	0.0	8.051082508298654E-5	8.051082508298654E-5
11	0.0	2.0127706270746635E-5	0.0	2.0127706270746634E-4	1.0063853135373317E-4
12	0.0	2.0127706270746635E-5	0.0	2.616601815197062E-4	1.207662376244798E-4
13	0.0	4.025541254149327E-5	0.0	3.2204330033194615E-4	1.207662376244798E-4
14	0.0	4.025541254149327E-5	0.0	4.025541254149327E-4	2.0127706270746634E-4
15	0.0	6.03831188122399E-5	0.0	6.03831188122399E-4	2.2140476897821297E-4
16	0.0	6.03831188122399E-5	0.0	0.0015095779703059975	2.415324752489596E-4
17	0.0	6.03831188122399E-5	0.0	0.002294558514865116	2.415324752489596E-4
18	0.0	6.03831188122399E-5	0.0	0.002797751171633782	2.616601815197062E-4
19	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.0036028594224636473	2.616601815197062E-4
20	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.004790394092437699	2.817878877904529E-4
21	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.007245974257468788	2.817878877904529E-4
22	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.010828705973661689	2.817878877904529E-4
23	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.016102165016597305	3.2204330033194615E-4
24	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.023187117623900123	3.2204330033194615E-4
25	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.026145890445699878	3.2204330033194615E-4
26	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.03292892745894149	3.2204330033194615E-4
27	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.06199333531389963	3.2204330033194615E-4
28	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.12052470514923085	3.2204330033194615E-4
29	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.19692947815298506	3.421710066026928E-4
30	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.31924554916031234	3.421710066026928E-4
31	2.0127706270746635E-5	6.03831188122399E-5	0.0	0.6883675544595349	3.421710066026928E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1590	0.0	19.430819	5
CAAGACG	1710	0.0	18.391813	4
CTTATAC	3940	0.0	16.668783	37
ACGGACC	1865	0.0	15.772118	8
AGACGGA	1925	0.0	15.568831	6
GACGGAC	1895	0.0	15.522428	7
CGGACCA	1945	0.0	15.218509	9
GTACCGT	250	0.0	14.799999	6
CGAGCCG	1495	0.0	14.725752	15
CGAACGA	1085	0.0	14.32258	16
CTAGCGG	1060	0.0	14.311321	29
GCGCAAG	2180	0.0	14.256881	1
CGCAAGA	2135	0.0	14.2107725	2
TACCGTC	1285	0.0	13.96498	7
GCAAGAC	2400	0.0	13.952084	3
TCTAGCG	1050	0.0	13.919047	28
AGAGCGA	2130	0.0	13.809859	15
TCTTATA	6335	0.0	13.754538	37
ACGAACG	1130	0.0	13.752213	15
TATACTG	740	0.0	13.750001	5
>>END_MODULE