FastQCFastQC Report
Thu 9 Feb 2017
SRR2727207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998865
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139770.34952417748536146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90280.22576406055218168No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC79180.1980061842547823No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG68510.1713236130752101No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62440.1561443059468124No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50940.1273861458188761No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.12220967699584756No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT44260.11068140584890962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG11150.017.7533655
CAAGACG11800.016.3050844
CGAACGA5800.015.94827616
GACGGAC11950.015.4811727
CGAACGT3250.015.3692314
ATACCGT11150.015.2645756
GCGCAAG13050.015.1685821
ACGAACG6150.015.04065115
TATACTG6400.015.031255
ACGGACC12200.015.0122968
CGAGCCG8850.014.84180715
TGTTACG2251.8189894E-1214.824
AGACGGA13500.014.7999996
TACCGTC10150.014.7635477
CGCAAGA13000.014.6576932
ACCGTCG9850.014.6497478
CGTCGTA9650.014.56994810
CCGTCGT10000.014.4300019
CGCATCG6450.014.34108513
CCGATAA6400.014.16406259
Produced by FastQC (version 0.11.5)