FastQCFastQC Report
Thu 9 Feb 2017
SRR2727206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6301883
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT526020.8347028975307856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231630.3675568080207138No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200650.31839689819693573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104350.16558542899003362No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76180.12088450388558468No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG64420.10222341481109694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG7850.016.4968151
AAGTACG14400.013.4895831
TATACCG4000.013.4124995
GAGTACT165300.012.69146911
GTACTTT169450.012.42431413
AGTACTT172350.012.23672812
TACTTTT176600.011.95271914
TACCGAC3153.6379788E-1211.7460327
GTATAAG8800.011.56251
TATACTG9600.011.5624995
CGGGAAT22400.011.479916
TTAGACT5000.011.4699994
ACTTTTT190300.011.41303215
GTATACT4000.011.0999994
TAGACAG11350.011.08375
GGGTAAG18900.011.0608451
TATGCGC2207.0222995E-710.93181810
TACACCG4250.010.8823535
CTTTTTT203800.010.74779216
TAGTACG3105.002221E-1010.7419361
Produced by FastQC (version 0.11.5)