##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727206.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6301883
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.091274147742826	31.0	31.0	33.0	26.0	34.0
2	30.420482417715466	31.0	31.0	34.0	26.0	34.0
3	31.21647783686241	31.0	31.0	34.0	28.0	34.0
4	35.19458073087044	35.0	35.0	37.0	33.0	37.0
5	35.05026021587516	35.0	35.0	37.0	32.0	37.0
6	35.09275656180859	36.0	35.0	37.0	32.0	37.0
7	34.970122104774084	36.0	35.0	37.0	32.0	37.0
8	34.96218828562828	36.0	35.0	37.0	32.0	37.0
9	36.399605482996115	38.0	35.0	39.0	32.0	39.0
10	36.188511433804784	38.0	35.0	39.0	32.0	39.0
11	36.25161289094069	38.0	35.0	39.0	32.0	39.0
12	36.046806803617265	37.0	35.0	39.0	30.0	39.0
13	36.059674386211235	37.0	35.0	39.0	30.0	39.0
14	36.89946766704492	38.0	36.0	40.0	31.0	41.0
15	36.87543929330329	38.0	36.0	40.0	31.0	41.0
16	36.6929895397931	38.0	36.0	40.0	30.0	41.0
17	37.19390252088146	39.0	36.0	40.0	32.0	41.0
18	37.362511966661394	39.0	36.0	41.0	32.0	41.0
19	37.49029710643628	39.0	36.0	41.0	32.0	41.0
20	37.512341311319176	39.0	36.0	41.0	32.0	41.0
21	37.463590326891186	39.0	36.0	41.0	32.0	41.0
22	37.4124091164498	39.0	36.0	41.0	32.0	41.0
23	37.2959004475329	39.0	36.0	41.0	31.0	41.0
24	37.196066794639	39.0	36.0	41.0	31.0	41.0
25	37.08745909754275	39.0	36.0	41.0	31.0	41.0
26	36.81218343787087	39.0	36.0	40.0	30.0	41.0
27	36.65891623186276	39.0	36.0	40.0	30.0	41.0
28	36.49170589171522	39.0	35.0	40.0	30.0	41.0
29	36.31434826701797	39.0	35.0	40.0	30.0	41.0
30	36.177753379426434	38.0	35.0	40.0	29.0	41.0
31	35.987905995715884	38.0	35.0	40.0	29.0	41.0
32	35.82873674423978	38.0	35.0	40.0	27.0	41.0
33	35.6862726585054	38.0	34.0	40.0	27.0	41.0
34	35.54706236215429	38.0	34.0	40.0	27.0	41.0
35	35.364139892790774	38.0	34.0	40.0	26.0	41.0
36	35.218557374042014	38.0	34.0	40.0	25.0	41.0
37	35.073916161248945	38.0	34.0	40.0	25.0	41.0
38	34.974529517606086	38.0	33.0	40.0	25.0	41.0
39	34.85414311881068	38.0	33.0	40.0	24.0	41.0
40	34.73625057780349	38.0	33.0	40.0	24.0	41.0
41	34.594872357992045	38.0	33.0	40.0	23.0	41.0
42	34.42869662924558	38.0	33.0	40.0	23.0	41.0
43	34.0847356258439	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	17.0
13	28.0
14	55.0
15	121.0
16	227.0
17	530.0
18	1076.0
19	2236.0
20	4507.0
21	8595.0
22	15057.0
23	24152.0
24	36227.0
25	50810.0
26	68973.0
27	90661.0
28	118302.0
29	152223.0
30	192929.0
31	241051.0
32	298578.0
33	366334.0
34	448821.0
35	546366.0
36	682614.0
37	869592.0
38	1039978.0
39	1041818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.43748749381733	28.235513099814767	14.14972318591126	22.177276220456648
2	20.111528570111506	25.78873647765279	30.37190630165619	23.727828650579514
3	19.8368328958186	25.326699972055973	30.666421448954225	24.170045683171203
4	15.918749999008233	17.27637596572326	32.37435541091448	34.43051862435402
5	13.601696508805382	39.609335812803884	32.92658718037133	13.862380498019403
6	34.517175263330024	37.71226790468817	12.589824342978122	15.180732489003685
7	28.59580223879117	31.620057052788823	20.042485714190505	19.741654994229503
8	28.4647144353521	35.79222273723584	17.806249338491366	17.93681348892069
9	26.81146254222746	14.88761374973163	18.3116220977127	39.98930161032821
10	16.718161857336927	29.746220296378084	32.429481791394736	21.10613605489026
11	35.60948370510846	24.083055810461733	19.255625659822627	21.051834824607184
12	21.102311801091833	27.30685415771762	28.782333788170934	22.808500253019616
13	31.717281961597827	20.707366353834242	23.201462166149387	24.373889518418544
14	21.953485966019997	22.511731810952377	25.19224809473613	30.342534128291497
15	25.289663422821402	29.562307012047036	21.771064299353064	23.376965265778498
16	23.756915195029805	27.081191447064313	24.419082359986692	24.742810997919193
17	23.829226915193445	27.628805549071604	25.424607216604944	23.11736031913001
18	23.483473114305674	26.379083838909732	26.288539473043215	23.848903573741374
19	23.887526950278197	27.200251099552307	26.76369904042966	22.148522909739835
20	23.73187506020026	26.92346081322043	26.289793066611995	23.054871059967315
21	23.075483946623574	27.484293186655478	26.28435024896527	23.155872617755676
22	23.713055288395548	27.117371109555666	25.528814165543857	23.64075943650493
23	23.42344343746147	26.94540663481058	25.599999238322894	24.031150689405056
24	23.47525334888001	27.064720179666935	25.77734305762262	23.68268341383044
25	23.97854736433539	26.35812185024698	25.607616009373707	24.05571477604392
26	23.788381980433467	26.799323313365225	26.13842561025014	23.273869095951163
27	24.052477648347327	27.240096333111865	25.803509839836757	22.903916178704048
28	23.41735002062082	27.59256241348816	25.951957533962467	23.038130031928553
29	23.551056089108606	27.611794760391454	26.004053074295413	22.833096076204527
30	23.253160999656767	27.684344504650436	25.987169231799445	23.075325263893347
31	23.394134737188867	26.975064437089674	26.14144058212442	23.489360243597034
32	22.83427032840819	27.358108679580372	25.858334723129577	23.949286268881856
33	23.045762671252387	27.475724319223318	25.776993955616124	23.701519053908175
34	23.588552818260826	27.27137269923926	25.842720342475417	23.2973541400245
35	23.314412533523708	27.54957526187014	26.009432418850047	23.1265797857561
36	23.39532485766556	27.616793266393554	25.86796676485425	23.119915111086637
37	22.955075490928664	27.30220475372202	26.272480780744424	23.470238974604893
38	23.15801483461372	27.60384475560717	26.167845388433904	23.070295021345206
39	22.754262495828627	27.740692742153417	26.150580707385394	23.354464054632558
40	23.319982297354617	27.485277019582878	26.554856699180228	22.639883983882278
41	23.40313204799264	27.118323205937017	26.267593352653485	23.210951393416856
42	22.70611815547829	27.569601022424568	26.448444060291187	23.275836761805955
43	23.313127203408886	26.840199984671248	26.337128125038184	23.509544686881682
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2362.0
1	2270.5
2	2179.0
3	2080.0
4	1981.0
5	1981.0
6	2329.0
7	2677.0
8	3875.5
9	5074.0
10	7829.5
11	10585.0
12	10585.0
13	30127.0
14	49669.0
15	78343.0
16	107017.0
17	81805.5
18	56594.0
19	56594.0
20	67406.0
21	78218.0
22	67123.0
23	56028.0
24	64335.5
25	72643.0
26	72643.0
27	88371.0
28	104099.0
29	118825.0
30	133551.0
31	152748.0
32	171945.0
33	171945.0
34	189350.0
35	206755.0
36	231765.0
37	256775.0
38	284367.5
39	311960.0
40	311960.0
41	340397.5
42	368835.0
43	392096.0
44	415357.0
45	439043.0
46	462729.0
47	462729.0
48	489757.0
49	516785.0
50	532640.5
51	548496.0
52	545637.5
53	542779.0
54	542779.0
55	508266.0
56	473753.0
57	423731.0
58	373709.0
59	338610.0
60	303511.0
61	303511.0
62	264931.5
63	226352.0
64	191347.5
65	156343.0
66	129627.0
67	102911.0
68	102911.0
69	84450.5
70	65990.0
71	54384.5
72	42779.0
73	35149.5
74	27520.0
75	27520.0
76	22397.0
77	17274.0
78	14292.5
79	11311.0
80	8895.0
81	6479.0
82	6479.0
83	5185.0
84	3891.0
85	3095.5
86	2300.0
87	1832.0
88	1364.0
89	1364.0
90	1018.5
91	673.0
92	494.5
93	316.0
94	257.0
95	198.0
96	198.0
97	136.0
98	74.0
99	58.0
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6301883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.06250851870011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.03828450091677	44.62171230672573
2	9.947597446521343	10.95479338279362
3	3.3683429532146327	5.564082376658527
4	1.6255301974509473	3.5802308137818826
5	0.9350827162246478	2.574400001390446
6	0.6117672158659812	2.021126252104922
7	0.416790241319332	1.6064661349209757
8	0.3018654911180921	1.329717694095323
9	0.24508632770889843	1.2145561206559332
>10	1.3666900159324231	13.960717611552079
>50	0.09941205392017793	3.7234843791733523
>100	0.039114580104417075	3.721333049237624
>500	0.002568381216090443	0.9797577579775771
>1k	0.0014884656689433623	1.5578090194354615
>5k	2.626704119754516E-4	0.8821000963753459
>10k+	1.1674240532242292E-4	1.7077130031212633
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	52602	0.8347028975307856	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23163	0.3675568080207138	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20065	0.31839689819693573	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10435	0.16558542899003362	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7618	0.12088450388558468	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	6442	0.10222341481109694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.347309209009435E-5
2	0.0	0.0	0.0	0.0	6.347309209009435E-5
3	0.0	0.0	0.0	0.0	6.347309209009435E-5
4	0.0	0.0	0.0	0.0	6.347309209009435E-5
5	0.0	0.0	0.0	0.0	6.347309209009435E-5
6	0.0	0.0	0.0	0.0	1.110779111576651E-4
7	1.5868273022523587E-5	0.0	0.0	0.0	1.110779111576651E-4
8	1.5868273022523587E-5	0.0	0.0	0.0	1.269461841801887E-4
9	1.5868273022523587E-5	0.0	0.0	1.5868273022523587E-5	2.3802409533785378E-4
10	1.5868273022523587E-5	0.0	0.0	3.173654604504717E-5	2.3802409533785378E-4
11	1.5868273022523587E-5	0.0	0.0	3.173654604504717E-5	2.6976064138290097E-4
12	1.5868273022523587E-5	0.0	0.0	3.173654604504717E-5	2.6976064138290097E-4
13	1.5868273022523587E-5	0.0	0.0	3.173654604504717E-5	2.6976064138290097E-4
14	1.5868273022523587E-5	0.0	0.0	4.760481906757075E-5	3.332337334729953E-4
15	1.5868273022523587E-5	1.5868273022523587E-5	0.0	9.52096381351415E-5	3.4910200649551886E-4
16	3.173654604504717E-5	1.5868273022523587E-5	0.0	1.110779111576651E-4	4.443116446306604E-4
17	3.173654604504717E-5	1.5868273022523587E-5	0.0	1.7455100324775943E-4	4.443116446306604E-4
18	3.173654604504717E-5	1.5868273022523587E-5	0.0	1.7455100324775943E-4	4.919164636982312E-4
19	6.347309209009435E-5	1.5868273022523587E-5	0.0	3.014971874279481E-4	4.919164636982312E-4
20	6.347309209009435E-5	1.5868273022523587E-5	0.0	5.236530097432784E-4	4.919164636982312E-4
21	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.0010155694734415095	4.919164636982312E-4
22	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.0018565879436352596	5.077847367207548E-4
23	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.0023802409533785376	7.140722860135613E-4
24	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.0031895228775272407	7.140722860135613E-4
25	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.0038718586174957548	7.299405590360849E-4
26	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.004903296363959788	7.458088320586085E-4
27	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.007664375869878891	7.61677105081132E-4
28	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.014329050539338797	7.934136511261793E-4
29	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.028356603891249648	7.934136511261793E-4
30	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.046636854413196815	7.934136511261793E-4
31	6.347309209009435E-5	1.5868273022523587E-5	0.0	0.09354346946777653	8.092819241487029E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGG	785	0.0	16.496815	1
AAGTACG	1440	0.0	13.489583	1
TATACCG	400	0.0	13.412499	5
GAGTACT	16530	0.0	12.691469	11
GTACTTT	16945	0.0	12.424314	13
AGTACTT	17235	0.0	12.236728	12
TACTTTT	17660	0.0	11.952719	14
TACCGAC	315	3.6379788E-12	11.746032	7
GTATAAG	880	0.0	11.5625	1
TATACTG	960	0.0	11.562499	5
CGGGAAT	2240	0.0	11.47991	6
TTAGACT	500	0.0	11.469999	4
ACTTTTT	19030	0.0	11.413032	15
GTATACT	400	0.0	11.099999	4
TAGACAG	1135	0.0	11.0837	5
GGGTAAG	1890	0.0	11.060845	1
TATGCGC	220	7.0222995E-7	10.931818	10
TACACCG	425	0.0	10.882353	5
CTTTTTT	20380	0.0	10.747792	16
TAGTACG	310	5.002221E-10	10.741936	1
>>END_MODULE