FastQCFastQC Report
Thu 9 Feb 2017
SRR2727205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5455963
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT309410.5671042857145475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT168050.30801161958026474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133610.244888024350605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76850.14085506078395327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC5600.013.2142853
CGTATAC1857.400013E-813.03
TATACCG3150.012.3333345
TTACACT8550.011.9005854
GGGTAAG14100.011.6773051
CTACACT11300.011.4601774
GAGTACT115400.011.41421211
GTATAAG7800.011.3846161
AAGTACG9100.011.3846151
ATACGGG5200.011.3846151
TACACAG18950.011.22691255
GTACTTT117800.011.19736813
CTAGTAC4150.011.1445783
TCGTATA1502.6582755E-411.12
AGTACTT120600.010.98341612
TCTACAC9150.010.9180333
TATACGA1706.989132E-510.8823535
CAAGACT19750.010.8658234
TAGACTG7500.010.85333255
TATACAG10600.010.8207555
Produced by FastQC (version 0.11.5)