##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727205.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5455963
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.14105539205453	31.0	31.0	33.0	26.0	34.0
2	30.458645155768103	31.0	31.0	34.0	26.0	34.0
3	31.26385681134568	31.0	31.0	34.0	28.0	34.0
4	35.236479059700365	35.0	35.0	37.0	33.0	37.0
5	35.08358561082618	35.0	35.0	37.0	32.0	37.0
6	35.11646486605573	36.0	35.0	37.0	32.0	37.0
7	35.00711973303338	36.0	35.0	37.0	32.0	37.0
8	34.9966255269693	36.0	35.0	37.0	32.0	37.0
9	36.442854359532866	38.0	35.0	39.0	32.0	39.0
10	36.24503758548216	38.0	35.0	39.0	32.0	39.0
11	36.299007709546416	38.0	35.0	39.0	32.0	39.0
12	36.09483513726175	38.0	35.0	39.0	30.0	39.0
13	36.107664586435064	38.0	35.0	39.0	31.0	39.0
14	36.99409196873219	39.0	36.0	40.0	31.0	41.0
15	36.943947933664504	38.0	36.0	40.0	31.0	41.0
16	36.77950638594873	38.0	36.0	40.0	31.0	41.0
17	37.262927369558774	39.0	36.0	40.0	32.0	41.0
18	37.41002954015634	39.0	36.0	41.0	32.0	41.0
19	37.53004758280069	39.0	36.0	41.0	32.0	41.0
20	37.534247208054744	39.0	36.0	41.0	32.0	41.0
21	37.4758536668962	39.0	36.0	41.0	32.0	41.0
22	37.423018814460434	39.0	36.0	41.0	32.0	41.0
23	37.30602810173016	39.0	36.0	41.0	31.0	41.0
24	37.21101187819639	39.0	36.0	41.0	31.0	41.0
25	37.09788464474558	39.0	36.0	41.0	31.0	41.0
26	36.83146641573632	39.0	36.0	40.0	30.0	41.0
27	36.68212815959346	39.0	36.0	40.0	30.0	41.0
28	36.52338991301811	39.0	35.0	40.0	30.0	41.0
29	36.35139332873042	39.0	35.0	40.0	30.0	41.0
30	36.22801895833971	39.0	35.0	40.0	29.0	41.0
31	36.048443327053356	38.0	35.0	40.0	29.0	41.0
32	35.89322361606924	38.0	35.0	40.0	27.0	41.0
33	35.74569695578947	38.0	34.0	40.0	27.0	41.0
34	35.60935970423553	38.0	34.0	40.0	27.0	41.0
35	35.44133693721896	38.0	34.0	40.0	26.0	41.0
36	35.3055788684784	38.0	34.0	40.0	25.0	41.0
37	35.16414975688068	38.0	34.0	40.0	25.0	41.0
38	35.08225990535493	38.0	34.0	40.0	25.0	41.0
39	34.97072542464089	38.0	33.0	40.0	24.0	41.0
40	34.86239019582794	38.0	33.0	40.0	24.0	41.0
41	34.73437759750204	38.0	33.0	40.0	24.0	41.0
42	34.59716112444311	38.0	33.0	40.0	23.0	41.0
43	34.267653024773075	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	11.0
13	15.0
14	43.0
15	95.0
16	209.0
17	412.0
18	906.0
19	1965.0
20	3889.0
21	7508.0
22	12765.0
23	20433.0
24	30768.0
25	43063.0
26	58376.0
27	77374.0
28	100395.0
29	129311.0
30	163762.0
31	204586.0
32	253526.0
33	310937.0
34	381165.0
35	467662.0
36	583900.0
37	744906.0
38	918349.0
39	939624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.35377714255027	28.07264272136743	13.815086355974188	22.758493780108115
2	20.383184416756492	24.85247792186274	30.650244512288666	24.114093149092103
3	19.546136951441937	25.102039731574425	30.45055474166522	24.901268575318415
4	16.02404561761141	17.2653846809445	32.50590225776824	34.204667443675845
5	13.611492599931488	39.154792655302096	33.37091912096911	13.862795623797302
6	34.742317717330565	37.82773086987577	12.827396373472475	14.602555039321196
7	28.82680839294548	31.68914451949179	20.017547772959603	19.466499314603123
8	27.897384934611907	35.92874438481346	17.946767600879994	18.22710307969464
9	26.658043685413556	14.808238252348852	18.33505835725059	40.198659704987
10	16.571556661949504	29.221917377372243	32.866608516223444	21.33991744445481
11	35.97857976676161	23.59290193133641	19.680155455599678	20.748362846302292
12	20.98901697097286	26.815467773516794	29.005017079477994	23.19049817603235
13	31.167201830364316	20.80149737085827	23.42770652953475	24.603594269242663
14	22.039244034462843	22.165179639231425	24.808104453787536	30.987471872518196
15	24.962009456442427	29.46755687309463	21.666385934068835	23.90404773639411
16	24.006962657188108	27.309441064757955	24.08920295097309	24.594393327080848
17	23.73729440613875	27.595641685986504	25.206420937971902	23.46064296990284
18	23.582051417870687	26.605385703678706	25.93903221117885	23.87353066727175
19	24.083722708530097	26.973808290122204	26.366161207471535	22.57630779387617
20	23.946001833223576	26.6453236578034	26.098619070547212	23.31005543842581
21	23.500837524008137	27.039809470848684	26.04552120313133	23.413831802011853
22	24.091109122257613	26.574776991706138	25.539176127110835	23.794937758925418
23	23.815649043074522	26.569773292084275	25.464982075574927	24.149595589266276
24	23.817976771470043	26.684015269165133	25.55609339726094	23.941914562103886
25	24.277858922430376	26.181372564293415	25.507504358075746	24.033264155200467
26	24.035500240745765	26.489255883883377	25.817642091781046	23.657601783589808
27	24.21693475560593	26.900054124267335	25.523395228303418	23.359615891823314
28	23.929542777324553	26.933137193195776	25.729206741321377	23.408113288158297
29	23.905257422016977	27.02514661481392	25.723928113148858	23.345667850020245
30	23.786213359584732	27.03660197109108	25.65704716106029	23.520137508263893
31	23.872320981648887	26.420010546259203	25.905949142250417	23.801719329841497
32	23.442497685559818	26.75179432118583	25.687729187313035	24.117978805941316
33	23.566563776183965	26.75918073491334	25.68355027334313	23.99070521555956
34	23.95192196134761	26.546367708138785	25.8152593776754	23.686450952838207
35	23.699061009028107	26.884126596899577	25.83745527599802	23.5793571180743
36	23.749354605227342	26.87622698321085	25.80593013552328	23.56848827603853
37	23.484488439529375	26.605404032248753	26.18371862125898	23.726388906962896
38	23.574133475611912	26.794848132217908	26.07970398626237	23.55131440590781
39	23.285733425978144	26.89851452438369	26.130914010963785	23.684838038674382
40	23.755403033341686	26.671331898695062	26.386432605939593	23.18683246202366
41	23.68705579564964	26.430109588353147	26.213135976178727	23.66969863981849
42	23.237419315343598	26.716750095262743	26.24869340206303	23.797137187330634
43	23.616509129552384	26.204319933987826	26.19893133439505	23.980239602064753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2547.0
1	2272.0
2	1997.0
3	1871.0
4	1745.0
5	1745.0
6	2120.5
7	2496.0
8	3330.0
9	4164.0
10	6321.0
11	8478.0
12	8478.0
13	21694.0
14	34910.0
15	50816.5
16	66723.0
17	53125.5
18	39528.0
19	39528.0
20	47799.0
21	56070.0
22	50362.0
23	44654.0
24	51462.0
25	58270.0
26	58270.0
27	71183.5
28	84097.0
29	96374.0
30	108651.0
31	125369.0
32	142087.0
33	142087.0
34	160298.5
35	178510.0
36	202808.5
37	227107.0
38	251859.5
39	276612.0
40	276612.0
41	301137.0
42	325662.0
43	347211.5
44	368761.0
45	390979.5
46	413198.0
47	413198.0
48	433299.0
49	453400.0
50	463760.0
51	474120.0
52	468850.0
53	463580.0
54	463580.0
55	437205.0
56	410830.0
57	374191.5
58	337553.0
59	306138.0
60	274723.0
61	274723.0
62	239355.5
63	203988.0
64	171865.5
65	139743.0
66	115770.5
67	91798.0
68	91798.0
69	75312.0
70	58826.0
71	48391.0
72	37956.0
73	31052.5
74	24149.0
75	24149.0
76	19741.0
77	15333.0
78	12652.0
79	9971.0
80	7912.5
81	5854.0
82	5854.0
83	4596.0
84	3338.0
85	2704.5
86	2071.0
87	1675.0
88	1279.0
89	1279.0
90	948.0
91	617.0
92	453.5
93	290.0
94	230.0
95	170.0
96	170.0
97	117.5
98	65.0
99	53.5
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5455963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.52443779057281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53022107708183	46.89980130400242
2	9.544381443778152	10.980703532242275
3	3.250418430713876	5.60935478432795
4	1.6376846242989966	3.7682754916426093
5	0.9331367025362273	2.6839082097572726
6	0.6047214363538808	2.087175638769879
7	0.4222354992274692	1.700220179579741
8	0.3197807824463585	1.4716167781165
9	0.24047426611481051	1.2449832265219563
>10	1.4026002145628582	14.77949028066364
>50	0.08471009429965551	3.2898299113781033
>100	0.027248736615526705	2.5943158057038276
>500	0.0012256042490330402	0.4572615710942196
>1k	9.998255379533025E-4	1.0592297537523974
>5k	6.450487341471913E-5	0.24011335165809528
>10k+	9.675731012207872E-5	1.1337201807889992
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30941	0.5671042857145475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16805	0.30801161958026474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13361	0.244888024350605	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7685	0.14085506078395327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4662856034764165E-4
2	0.0	0.0	0.0	0.0	1.4662856034764165E-4
3	0.0	0.0	0.0	0.0	1.4662856034764165E-4
4	0.0	0.0	0.0	0.0	1.4662856034764165E-4
5	0.0	0.0	0.0	0.0	1.4662856034764165E-4
6	0.0	0.0	0.0	0.0	1.4662856034764165E-4
7	0.0	0.0	0.0	1.8328570043455207E-5	1.8328570043455207E-4
8	0.0	0.0	0.0	1.8328570043455207E-5	1.8328570043455207E-4
9	0.0	0.0	0.0	1.8328570043455207E-5	2.932571206952833E-4
10	0.0	0.0	0.0	1.8328570043455207E-5	2.932571206952833E-4
11	0.0	0.0	0.0	1.8328570043455207E-5	3.115856907387385E-4
12	0.0	0.0	0.0	1.8328570043455207E-5	3.115856907387385E-4
13	0.0	0.0	0.0	1.8328570043455207E-5	3.2991426078219374E-4
14	0.0	0.0	0.0	7.331428017382083E-5	4.582142510863802E-4
15	0.0	0.0	0.0	7.331428017382083E-5	4.948713911732906E-4
16	1.8328570043455207E-5	0.0	0.0	7.331428017382083E-5	5.865142413905666E-4
17	1.8328570043455207E-5	0.0	0.0	1.0997142026073124E-4	6.048428114340218E-4
18	1.8328570043455207E-5	0.0	0.0	1.4662856034764165E-4	6.598285215643875E-4
19	1.8328570043455207E-5	0.0	0.0	2.382714105649177E-4	6.598285215643875E-4
20	1.8328570043455207E-5	0.0	0.0	3.6657140086910413E-4	6.781570916078426E-4
21	1.8328570043455207E-5	0.0	0.0	8.247856519554843E-4	6.781570916078426E-4
22	1.8328570043455207E-5	0.0	0.0	0.00133798561317223	6.964856616512978E-4
23	1.8328570043455207E-5	0.0	0.0	0.0017595427241716998	8.797713620858499E-4
24	1.8328570043455207E-5	0.0	0.0	0.0023643855356057218	8.797713620858499E-4
25	1.8328570043455207E-5	0.0	0.0	0.0027676140765617364	8.797713620858499E-4
26	1.8328570043455207E-5	0.0	0.0	0.0035007568782999443	8.980999321293051E-4
27	1.8328570043455207E-5	0.0	0.0	0.006909870906382613	8.980999321293051E-4
28	1.8328570043455207E-5	0.0	0.0	0.012701699040114459	9.164285021727604E-4
29	1.8328570043455207E-5	0.0	0.0	0.024358669587751968	9.164285021727604E-4
30	1.8328570043455207E-5	0.0	0.0	0.04045115408590564	9.164285021727604E-4
31	1.8328570043455207E-5	0.0	0.0	0.08064570819120291	9.164285021727604E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	560	0.0	13.214285	3
CGTATAC	185	7.400013E-8	13.0	3
TATACCG	315	0.0	12.333334	5
TTACACT	855	0.0	11.900585	4
GGGTAAG	1410	0.0	11.677305	1
CTACACT	1130	0.0	11.460177	4
GAGTACT	11540	0.0	11.414212	11
GTATAAG	780	0.0	11.384616	1
AAGTACG	910	0.0	11.384615	1
ATACGGG	520	0.0	11.384615	1
TACACAG	1895	0.0	11.2269125	5
GTACTTT	11780	0.0	11.197368	13
CTAGTAC	415	0.0	11.144578	3
TCGTATA	150	2.6582755E-4	11.1	2
AGTACTT	12060	0.0	10.983416	12
TCTACAC	915	0.0	10.918033	3
TATACGA	170	6.989132E-5	10.882353	5
CAAGACT	1975	0.0	10.865823	4
TAGACTG	750	0.0	10.8533325	5
TATACAG	1060	0.0	10.820755	5
>>END_MODULE