FastQCFastQC Report
Thu 9 Feb 2017
SRR2727204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199469
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT404660.7782717812145817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT198250.3812889354662947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190030.36547962878517015No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG128970.24804455993487026No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG125600.24156312885027295No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG112490.21634901563986628No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG111400.21425264772229627No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG105610.20311689520602969No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT105000.20194369848151802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77130.1483420710845665No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73180.1407451414750237No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT68730.13218657520604507No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG66360.12762841744031939No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA63540.12220478668110146No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA62620.1204353752277396No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA59370.1141847369414069No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT58160.11185757622557227No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52280.10054872911060726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG8300.017.8313261
AAGTACG12350.017.0769231
TATACCG2551.8189894E-1213.7843135
CGTATAC950.001246106913.6315793
AATCAAC15200.013.5098691
ACCGAAC3600.012.8472228
TCTATAC4450.012.471913
GAGTACT151400.012.28038311
GTACTTT152950.012.09545613
CTTACAC7850.012.0191093
AGTACTT155500.011.944694512
CTAGAAC5500.011.7727283
TACTTTT157900.011.72799214
TATTAGG5150.011.4951452
GTATAAG7250.011.48275851
ACTATAC5650.011.4601773
AATACCG1801.0048221E-511.3055565
TAGTACT4600.011.260874
CTACACT8250.011.2121224
ACTTTTT169150.011.19952715
Produced by FastQC (version 0.11.5)