##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727204.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5199469
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.04639743019912	31.0	30.0	33.0	26.0	34.0
2	30.370393976769552	31.0	31.0	34.0	26.0	34.0
3	31.170370666696925	31.0	31.0	34.0	28.0	34.0
4	35.14459688095073	35.0	35.0	37.0	33.0	37.0
5	34.9959920907308	35.0	35.0	37.0	32.0	37.0
6	35.0641280869258	35.0	35.0	37.0	32.0	37.0
7	34.9207871034523	35.0	35.0	37.0	32.0	37.0
8	34.91820972487768	35.0	35.0	37.0	32.0	37.0
9	36.345912053711636	38.0	35.0	39.0	32.0	39.0
10	36.1333545790926	38.0	35.0	39.0	31.0	39.0
11	36.209794884823815	38.0	35.0	39.0	32.0	39.0
12	36.01198910888785	37.0	35.0	39.0	30.0	39.0
13	36.011051320817565	37.0	35.0	39.0	30.0	39.0
14	36.822379169872924	38.0	36.0	40.0	31.0	41.0
15	36.80432674952	38.0	36.0	40.0	31.0	41.0
16	36.568800390963	38.0	35.0	40.0	30.0	41.0
17	37.10058104010236	39.0	36.0	40.0	31.0	41.0
18	37.277957614517945	39.0	36.0	40.0	32.0	41.0
19	37.40713907516325	39.0	36.0	41.0	32.0	41.0
20	37.44694621700793	39.0	36.0	41.0	32.0	41.0
21	37.39678013274048	39.0	36.0	41.0	32.0	41.0
22	37.359769237974106	39.0	36.0	41.0	31.0	41.0
23	37.24177161167804	39.0	36.0	41.0	31.0	41.0
24	37.14018277635659	39.0	36.0	41.0	31.0	41.0
25	37.028981805642076	39.0	36.0	40.0	31.0	41.0
26	36.74755960656751	39.0	36.0	40.0	30.0	41.0
27	36.58864299412113	39.0	35.0	40.0	30.0	41.0
28	36.41232960519622	39.0	35.0	40.0	30.0	41.0
29	36.223956715579995	38.0	35.0	40.0	29.0	41.0
30	36.0808763356412	38.0	35.0	40.0	29.0	41.0
31	35.90834217878787	38.0	35.0	40.0	28.0	41.0
32	35.73192877965038	38.0	34.0	40.0	27.0	41.0
33	35.5926634046669	38.0	34.0	40.0	27.0	41.0
34	35.45091392986476	38.0	34.0	40.0	26.0	41.0
35	35.25852428392207	38.0	34.0	40.0	26.0	41.0
36	35.099839233583275	38.0	34.0	40.0	25.0	41.0
37	34.949139229409774	38.0	33.0	40.0	25.0	41.0
38	34.85613473221977	38.0	33.0	40.0	24.0	41.0
39	34.737168160825654	38.0	33.0	40.0	24.0	41.0
40	34.60382935257427	38.0	33.0	40.0	24.0	41.0
41	34.446341347549144	38.0	33.0	40.0	23.0	41.0
42	34.25708529082489	38.0	33.0	40.0	22.0	41.0
43	33.89935087602215	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	19.0
13	23.0
14	39.0
15	79.0
16	173.0
17	431.0
18	888.0
19	1918.0
20	3665.0
21	7310.0
22	12785.0
23	20414.0
24	30431.0
25	43299.0
26	59051.0
27	77316.0
28	100830.0
29	130343.0
30	164205.0
31	204567.0
32	251891.0
33	308539.0
34	375214.0
35	456450.0
36	569462.0
37	718720.0
38	859713.0
39	801687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.16786637250842	27.919581788063358	14.118922528435116	21.793629310993104
2	20.095782857826443	26.013252507131018	29.984465721403474	23.906498913639066
3	20.921501791817587	25.36012812077541	29.834777358995694	23.883592728411305
4	16.399174608022474	17.03071986774034	31.504178599776246	35.06592692446094
5	14.087669337003453	39.37303982387432	32.32632024539429	14.212970593727936
6	35.27606376728085	36.47028186916779	12.149009831580878	16.10464453197048
7	28.77441908010222	31.091540309212345	20.02198686058134	20.112053750104096
8	28.964996233269204	35.47581493417886	17.787720246048202	17.771468586503737
9	26.970523336132977	15.034342930018429	18.175952198195624	39.81918153565297
10	17.14034644691602	29.580347531642175	32.167361705589556	21.111944315852256
11	35.267620597411	23.98298749353059	18.542951212902704	22.206440696155706
12	21.579376663270807	26.99600670760803	28.35591480591576	23.06870182320541
13	32.488509884374736	20.279840114442454	22.94674706205576	24.284902939127054
14	22.225865756676306	22.739033543617627	25.180648254658312	29.85445244504775
15	26.47930009775998	28.628038747802904	21.816554728954053	23.076106425483065
16	24.044243748736648	26.425467677564768	24.821842384289628	24.708446189408956
17	24.407396216805985	27.547255306263008	25.265849262684327	22.779499214246684
18	23.90867221248939	25.694681514593125	26.217888788258954	24.17875748465853
19	23.99271925652408	27.561776019820485	26.638777921360816	21.80672680229462
20	24.06909244001647	26.37665500073181	26.125725530818627	23.428527028433095
21	22.757746993010247	27.680365052662108	26.188078051816444	23.3738099025112
22	23.800045735439525	27.20550887023271	24.9408545372614	24.053590857066364
23	23.35890453428994	26.643124519061466	25.290832583096467	24.707138363552126
24	23.494726096068653	27.212009534050495	25.592228744896833	23.701035624984012
25	24.28092176335699	25.860236881881594	24.921583338606307	24.937258016155113
26	24.050167430558776	26.412043229799043	26.138303738324048	23.399485601318133
27	24.750373547760358	26.862108419148186	25.610692168758003	22.776825864333457
28	23.586158509647813	27.75841148394192	25.692758241274255	22.962671765136015
29	23.900959886480717	27.55994891016756	25.9183389688447	22.620752234507023
30	23.099801152771562	27.611608031512446	26.054795210818643	23.23379560489735
31	23.690880741860372	26.722824965395503	26.003328416805637	23.582965875938484
32	22.6778926848107	27.096824694983273	25.750360277174455	24.474922343031565
33	23.106628773053554	27.700232466046053	25.340510732922922	23.85262802797747
34	24.153312578649857	27.26599581611122	25.025478563291752	23.55521304194717
35	23.457837713812697	27.54235095929988	25.81925192745644	23.18055939943098
36	23.82166332754364	27.75135307086166	25.3095267997559	23.117456801838802
37	22.743572468650164	27.168774349842263	26.19448255196829	23.893170629539284
38	23.528633404680363	27.512771015655634	25.983076348757923	22.97551923090608
39	22.769344331123044	27.603376421707676	25.61026904862785	24.017010198541428
40	23.714748563747566	27.43547466097019	26.519573441057155	22.33020333422509
41	24.181565463704082	26.703976886870564	25.764996387131074	23.349461262294284
42	22.669430282207664	27.55167883489641	26.55471164459294	23.224179238302987
43	24.057091214506713	26.51645773828058	26.068546615048575	23.35790443216413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1325.0
1	1556.5
2	1788.0
3	1782.5
4	1777.0
5	1777.0
6	2063.5
7	2350.0
8	3295.5
9	4241.0
10	6719.0
11	9197.0
12	9197.0
13	26610.5
14	44024.0
15	64626.5
16	85229.0
17	64695.5
18	44162.0
19	44162.0
20	51573.5
21	58985.0
22	49734.0
23	40483.0
24	46284.5
25	52086.0
26	52086.0
27	64438.0
28	76790.0
29	87658.0
30	98526.0
31	113425.5
32	128325.0
33	128325.0
34	140917.5
35	153510.0
36	173247.5
37	192985.0
38	214556.0
39	236127.0
40	236127.0
41	259056.0
42	281985.0
43	302460.0
44	322935.0
45	349229.5
46	375524.0
47	375524.0
48	415161.0
49	454798.0
50	481296.0
51	507794.0
52	509493.5
53	511193.0
54	511193.0
55	470567.0
56	429941.0
57	368286.0
58	306631.0
59	276504.0
60	246377.0
61	246377.0
62	214681.5
63	182986.0
64	154020.5
65	125055.0
66	103603.5
67	82152.0
68	82152.0
69	67226.0
70	52300.0
71	42899.5
72	33499.0
73	27401.5
74	21304.0
75	21304.0
76	17163.0
77	13022.0
78	10711.0
79	8400.0
80	6687.0
81	4974.0
82	4974.0
83	3945.5
84	2917.0
85	2323.5
86	1730.0
87	1392.0
88	1054.0
89	1054.0
90	779.0
91	504.0
92	384.5
93	265.0
94	198.5
95	132.0
96	132.0
97	91.5
98	51.0
99	43.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5199469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.9553783125065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66813936825028	43.24767216324199
2	9.413334129700134	9.969733400405993
3	3.2021827418933793	5.087183955682296
4	1.5523292606446626	3.288167330520464
5	0.9194642084121669	2.4345287500637807
6	0.5995375492816014	1.9049242640856123
7	0.43622400886767315	1.6170285193010072
8	0.3324102359802586	1.408232784102732
9	0.24905965617971434	1.187014348384161
>10	1.482830713232996	14.659835186115867
>50	0.09811687500323844	3.532488848071427
>100	0.039698238305960774	3.666288372183272
>500	0.0032074730713876117	1.143539563553468
>1k	0.0028019269087956205	2.8249633105834646
>5k	3.318071339355431E-4	1.1350262409530185
>10k+	3.318071339355431E-4	2.893372962751403
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40466	0.7782717812145817	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19825	0.3812889354662947	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19003	0.36547962878517015	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	12897	0.24804455993487026	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	12560	0.24156312885027295	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	11249	0.21634901563986628	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	11140	0.21425264772229627	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	10561	0.20311689520602969	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	10500	0.20194369848151802	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7713	0.1483420710845665	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7318	0.1407451414750237	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	6873	0.13218657520604507	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG	6636	0.12762841744031939	No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA	6354	0.12220478668110146	No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA	6262	0.1204353752277396	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA	5937	0.1141847369414069	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT	5816	0.11185757622557227	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5228	0.10054872911060726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9232733188716003E-5	0.0	0.0	0.0	3.8465466377432006E-5
2	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	3.8465466377432006E-5
3	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	3.8465466377432006E-5
4	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	3.8465466377432006E-5
5	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	3.8465466377432006E-5
6	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	5.7698199566148006E-5
7	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	5.7698199566148006E-5
8	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	5.7698199566148006E-5
9	1.9232733188716003E-5	0.0	0.0	1.9232733188716003E-5	2.6925826464202404E-4
10	1.9232733188716003E-5	0.0	0.0	5.7698199566148006E-5	2.6925826464202404E-4
11	1.9232733188716003E-5	0.0	0.0	5.7698199566148006E-5	2.6925826464202404E-4
12	1.9232733188716003E-5	0.0	0.0	7.693093275486401E-5	2.6925826464202404E-4
13	1.9232733188716003E-5	0.0	0.0	9.616366594358E-5	2.6925826464202404E-4
14	1.9232733188716003E-5	0.0	0.0	1.1539639913229601E-4	4.0388739696303603E-4
15	1.9232733188716003E-5	0.0	0.0	1.73094598698444E-4	4.0388739696303603E-4
16	1.9232733188716003E-5	0.0	0.0	3.65421930585604E-4	4.8081832971790005E-4
17	1.9232733188716003E-5	0.0	0.0	5.769819956614801E-4	4.8081832971790005E-4
18	1.9232733188716003E-5	0.0	0.0	7.6930932754864E-4	5.385165292840481E-4
19	1.9232733188716003E-5	0.0	0.0	0.0011539639913229602	5.385165292840481E-4
20	1.9232733188716003E-5	0.0	0.0	0.001577084121474712	5.577492624727641E-4
21	1.9232733188716003E-5	0.0	0.0	0.0033272628416478683	6.154474620389121E-4
22	1.9232733188716003E-5	0.0	0.0	0.005865983622558381	6.154474620389121E-4
23	1.9232733188716003E-5	0.0	0.0	0.007635395075920252	9.039384598696521E-4
24	1.9232733188716003E-5	0.0	0.0	0.011039588850322985	9.039384598696521E-4
25	1.9232733188716003E-5	0.0	0.0	0.013712938763554509	9.039384598696521E-4
26	1.9232733188716003E-5	0.0	0.0	0.017155598004334673	9.039384598696521E-4
27	1.9232733188716003E-5	0.0	0.0	0.021963781301513673	9.039384598696521E-4
28	3.8465466377432006E-5	0.0	0.0	0.02934915084598062	9.231711930583681E-4
29	3.8465466377432006E-5	0.0	0.0	0.0429082277440254	9.231711930583681E-4
30	3.8465466377432006E-5	0.0	0.0	0.06312183032536592	9.231711930583681E-4
31	3.8465466377432006E-5	0.0	0.0	0.10647241093273178	9.424039262470841E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGG	830	0.0	17.831326	1
AAGTACG	1235	0.0	17.076923	1
TATACCG	255	1.8189894E-12	13.784313	5
CGTATAC	95	0.0012461069	13.631579	3
AATCAAC	1520	0.0	13.509869	1
ACCGAAC	360	0.0	12.847222	8
TCTATAC	445	0.0	12.47191	3
GAGTACT	15140	0.0	12.280383	11
GTACTTT	15295	0.0	12.095456	13
CTTACAC	785	0.0	12.019109	3
AGTACTT	15550	0.0	11.9446945	12
CTAGAAC	550	0.0	11.772728	3
TACTTTT	15790	0.0	11.727992	14
TATTAGG	515	0.0	11.495145	2
GTATAAG	725	0.0	11.4827585	1
ACTATAC	565	0.0	11.460177	3
AATACCG	180	1.0048221E-5	11.305556	5
TAGTACT	460	0.0	11.26087	4
CTACACT	825	0.0	11.212122	4
ACTTTTT	16915	0.0	11.199527	15
>>END_MODULE