##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727203.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1278004
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.336650746007056	31.0	30.0	31.0	27.0	34.0
2	30.542984216011842	31.0	30.0	31.0	27.0	34.0
3	30.411717803700146	31.0	30.0	31.0	27.0	34.0
4	34.18033668126235	35.0	33.0	37.0	30.0	37.0
5	33.634882989411615	35.0	33.0	37.0	28.0	37.0
6	34.04671972857675	35.0	33.0	37.0	30.0	37.0
7	34.06140669356277	35.0	33.0	37.0	30.0	37.0
8	34.04062350352581	35.0	33.0	37.0	30.0	37.0
9	35.58402790601594	37.0	35.0	39.0	30.0	39.0
10	35.08557328459066	37.0	34.0	39.0	29.0	39.0
11	35.39589469203539	37.0	34.0	39.0	30.0	39.0
12	35.10037057786987	37.0	34.0	39.0	29.0	39.0
13	35.267634530095364	37.0	34.0	39.0	30.0	39.0
14	35.98050788573432	38.0	34.0	40.0	29.0	41.0
15	35.93297438818658	38.0	34.0	40.0	29.0	41.0
16	35.77400931452484	38.0	34.0	40.0	28.0	41.0
17	35.68487970303692	38.0	34.0	40.0	27.0	41.0
18	35.56063596045083	38.0	33.0	40.0	27.0	41.0
19	35.53346859634242	38.0	34.0	40.0	27.0	41.0
20	35.38807625015258	38.0	33.0	40.0	27.0	41.0
21	35.25135680326509	38.0	33.0	40.0	27.0	41.0
22	35.554150065258014	38.0	34.0	40.0	27.0	41.0
23	35.5316462233295	38.0	34.0	40.0	27.0	41.0
24	35.4581472358459	38.0	34.0	40.0	27.0	41.0
25	35.416438446202044	38.0	34.0	40.0	27.0	41.0
26	35.148008926419635	38.0	33.0	40.0	27.0	41.0
27	34.9849914397764	38.0	33.0	40.0	26.0	41.0
28	34.77670648918157	37.0	33.0	40.0	25.0	41.0
29	34.61733453103433	37.0	33.0	40.0	25.0	41.0
30	34.45622001183095	37.0	33.0	40.0	25.0	41.0
31	34.3067470837337	37.0	32.0	39.0	24.0	40.0
32	34.1328736060294	37.0	32.0	39.0	24.0	40.0
33	33.96075990372487	37.0	32.0	39.0	24.0	40.0
34	33.80559450518152	37.0	31.0	39.0	24.0	40.0
35	33.63772805092942	36.0	31.0	39.0	23.0	40.0
36	33.463014982738706	36.0	31.0	39.0	23.0	40.0
37	33.302434108187455	36.0	31.0	39.0	22.0	40.0
38	33.132325876914315	36.0	30.0	39.0	21.0	40.0
39	32.97281229166732	36.0	30.0	39.0	21.0	40.0
40	32.77355391688915	36.0	30.0	39.0	19.0	40.0
41	32.62896047273718	36.0	30.0	39.0	19.0	40.0
42	32.414125464396044	35.0	30.0	39.0	18.0	40.0
43	32.23202822526377	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	10.0
13	6.0
14	39.0
15	61.0
16	115.0
17	270.0
18	574.0
19	1109.0
20	1941.0
21	3474.0
22	5628.0
23	8835.0
24	12783.0
25	17793.0
26	23815.0
27	30991.0
28	38798.0
29	48080.0
30	58346.0
31	69961.0
32	82410.0
33	97038.0
34	115213.0
35	137173.0
36	157769.0
37	166807.0
38	142115.0
39	56846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.07218443760739	26.41196741168259	15.548464637043388	20.967383513666622
2	27.201949289673582	25.063380083317423	26.42730382690508	21.30736680010391
3	28.021273798830048	23.81862654576981	27.399366512154895	20.760733143245247
4	27.019711988381882	18.02701712983684	26.891699869483976	28.0615710122973
5	15.752298114872879	39.17749866197602	31.39669359407326	13.673509629077843
6	35.64926244362302	36.67555031126663	12.678520568010743	14.9966666770996
7	29.920798369958153	31.507021887255437	19.12286659509673	19.44931314768968
8	28.663916544862143	35.74135918197439	17.640789856682765	17.9539344164807
9	27.656642702213762	14.055355069311206	17.75088340881562	40.537118819659405
10	17.557222043123495	29.80092394077014	31.614611534862174	21.02724248124419
11	37.82539021787099	22.151417366455817	18.977718379598187	21.045474036075003
12	21.609322036550747	26.247648677155937	28.379488640098156	23.76354064619516
13	32.58651772607911	20.1619869734367	22.86463892131793	24.386856379166264
14	22.546721293517077	21.761512483529003	24.681065160985412	31.01070106196851
15	26.48700630044976	28.548110960529076	21.109245354474634	23.855637384546526
16	25.04147091871387	26.687005674473635	24.132397081699274	24.13912632511322
17	25.08935809277592	27.034891909571492	24.5589215683206	23.31682842933199
18	24.982785656382923	25.617916688836655	25.414865681171577	23.984431973608846
19	25.864473037643076	25.90508323917609	25.808056938788926	22.42238678439191
20	25.210484474226995	25.39851205473535	25.958056469306822	23.432947001730824
21	25.025743268409173	25.33959205135508	26.07863512164281	23.556029558592932
22	25.205398418158314	25.640843064653946	25.31103189035402	23.84272662683372
23	24.95586868272713	25.58106234409282	25.646946331936366	23.81612264124369
24	24.786385645115352	25.73904307028773	25.609231270011673	23.865340014585243
25	25.4992942119117	25.467291182187225	25.438809268202604	23.594605337698475
26	25.10039092209414	25.614239079063918	25.557822980209764	23.72754701863218
27	25.416978350615494	26.104613131101313	25.135758573525592	23.342649944757607
28	24.94984366246115	26.355003583713355	25.39256528148582	23.30258747233968
29	24.91518023417767	26.544596104550532	25.700389044165746	22.83983461710605
30	25.202816266615752	25.934269376308684	25.3098581851074	23.553056171968162
31	25.387635719450017	25.64178202885124	25.787086738382666	23.18349551331608
32	24.860955051783876	25.934191129292238	25.687791274518702	23.51706254440518
33	25.070891796895783	25.76674251410794	25.174882081746226	23.987483607250056
34	25.108137376721825	26.081295520201813	25.47433341366694	23.336233689409422
35	25.19467857690586	26.074566276787863	25.43677484577513	23.293980300531143
36	25.20054710313896	26.01533328534183	25.199060409826572	23.58505920169264
37	24.806495128340757	26.32933856232062	25.732626814939547	23.131539494399078
38	25.349842410508888	25.920967383513666	25.42832416799947	23.300866037977972
39	24.675118387735875	26.54866494940548	25.490921781152487	23.28529488170616
40	25.452424249063384	26.013064121865032	25.750232393638832	22.784279235432752
41	24.933255294975602	25.902501087633528	25.337948864009814	23.826294753381053
42	24.87910835959825	26.241310668824198	25.347025517917004	23.532555453660553
43	25.192096425363303	25.916742044625842	25.28740129138876	23.6037602386221
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	250.0
1	248.0
2	246.0
3	230.5
4	215.0
5	215.0
6	387.0
7	559.0
8	618.5
9	678.0
10	1088.0
11	1498.0
12	1498.0
13	4715.0
14	7932.0
15	10219.5
16	12507.0
17	9329.0
18	6151.0
19	6151.0
20	8795.0
21	11439.0
22	9677.5
23	7916.0
24	9076.5
25	10237.0
26	10237.0
27	12595.5
28	14954.0
29	17250.5
30	19547.0
31	23111.5
32	26676.0
33	26676.0
34	30778.5
35	34881.0
36	40118.5
37	45356.0
38	51296.0
39	57236.0
40	57236.0
41	63755.5
42	70275.0
43	76805.0
44	83335.0
45	90399.0
46	97463.0
47	97463.0
48	103849.5
49	110236.0
50	114350.5
51	118465.0
52	118483.0
53	118501.0
54	118501.0
55	111097.5
56	103694.0
57	95062.5
58	86431.0
59	78988.0
60	71545.0
61	71545.0
62	62487.0
63	53429.0
64	45232.5
65	37036.0
66	30989.5
67	24943.0
68	24943.0
69	20688.0
70	16433.0
71	13432.0
72	10431.0
73	8642.0
74	6853.0
75	6853.0
76	5601.5
77	4350.0
78	3505.5
79	2661.0
80	2149.0
81	1637.0
82	1637.0
83	1267.0
84	897.0
85	716.0
86	535.0
87	431.5
88	328.0
89	328.0
90	241.5
91	155.0
92	106.5
93	58.0
94	41.5
95	25.0
96	25.0
97	16.5
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1278004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.43427475510714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.10837057889819	58.24329616155796
2	8.23874977722571	11.27625731786484
3	2.7142374394543882	5.572406120466603
4	1.2606967897912638	3.450994819818276
5	0.7165584641139443	2.4518579405635617
6	0.47179871664273276	1.9372321802301424
7	0.30626245855786327	1.46711944652864
8	0.22825688804593453	1.249647567302497
9	0.17153841380828216	1.056519624745012
>10	0.7429543358423839	8.330645826081929
>50	0.026467888675615777	1.2134052433165903
>100	0.011928948188108517	1.5508979620036507
>500	0.0014911005170505816	0.6739506891528992
>1k	4.588001590924866E-4	0.59686944331687
>5k	2.294000795462433E-4	0.9288996570504786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6249	0.4889656057414531	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5585	0.4370095868244544	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3634	0.2843496577475501	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1613	0.12621243751975736	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.824701644126309E-5
2	0.0	0.0	0.0	0.0	7.824701644126309E-5
3	0.0	0.0	0.0	0.0	7.824701644126309E-5
4	0.0	0.0	0.0	7.824701644126309E-5	1.5649403288252618E-4
5	7.824701644126309E-5	0.0	0.0	2.3474104932378928E-4	2.3474104932378928E-4
6	7.824701644126309E-5	0.0	0.0	3.1298806576505236E-4	3.1298806576505236E-4
7	7.824701644126309E-5	0.0	0.0	3.9123508220631546E-4	3.1298806576505236E-4
8	7.824701644126309E-5	0.0	0.0	3.9123508220631546E-4	3.9123508220631546E-4
9	7.824701644126309E-5	0.0	0.0	3.9123508220631546E-4	4.6948209864757857E-4
10	7.824701644126309E-5	0.0	0.0	3.9123508220631546E-4	4.6948209864757857E-4
11	7.824701644126309E-5	0.0	0.0	3.9123508220631546E-4	4.6948209864757857E-4
12	7.824701644126309E-5	0.0	0.0	3.9123508220631546E-4	4.6948209864757857E-4
13	7.824701644126309E-5	0.0	0.0	3.9123508220631546E-4	4.6948209864757857E-4
14	7.824701644126309E-5	0.0	0.0	4.6948209864757857E-4	7.042231479713678E-4
15	7.824701644126309E-5	0.0	0.0	4.6948209864757857E-4	7.042231479713678E-4
16	7.824701644126309E-5	0.0	0.0	4.6948209864757857E-4	7.824701644126309E-4
17	7.824701644126309E-5	0.0	0.0	4.6948209864757857E-4	7.824701644126309E-4
18	7.824701644126309E-5	0.0	0.0	4.6948209864757857E-4	7.824701644126309E-4
19	7.824701644126309E-5	0.0	0.0	4.6948209864757857E-4	7.824701644126309E-4
20	7.824701644126309E-5	0.0	0.0	4.6948209864757857E-4	8.60717180853894E-4
21	1.5649403288252618E-4	0.0	0.0	5.477291150888416E-4	9.389641972951571E-4
22	1.5649403288252618E-4	0.0	0.0	5.477291150888416E-4	9.389641972951571E-4
23	1.5649403288252618E-4	0.0	0.0	7.042231479713678E-4	0.0010954582301776832
24	1.5649403288252618E-4	0.0	0.0	7.042231479713678E-4	0.0010954582301776832
25	1.5649403288252618E-4	0.0	0.0	7.042231479713678E-4	0.0010954582301776832
26	1.5649403288252618E-4	0.0	0.0	9.389641972951571E-4	0.0010954582301776832
27	1.5649403288252618E-4	0.0	0.0	0.0014866933123839987	0.0010954582301776832
28	1.5649403288252618E-4	0.0	0.0	0.0023474104932378927	0.0010954582301776832
29	1.5649403288252618E-4	0.0	0.0	0.005712032200212206	0.0010954582301776832
30	1.5649403288252618E-4	0.0	0.0	0.00938964197295157	0.0010954582301776832
31	1.5649403288252618E-4	0.0	0.0	0.021361435488464826	0.0010954582301776832
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACAC	145	1.546141E-10	17.862068	5
CGGTAAC	115	6.406481E-8	17.695652	1
GTATACT	140	1.869921E-9	17.178572	4
TAATACC	95	7.060661E-5	15.578948	4
TATACAC	215	0.0	15.488372	3
TGTTACG	145	5.3461918E-8	15.310345	22
GGTTATA	145	5.3461918E-8	15.310345	1
GGACCGT	85	5.364735E-4	15.235293	6
TGTATAC	135	3.9728548E-7	15.074073	3
GACCGTG	175	2.237357E-9	14.8	7
CTTATAC	175	2.237357E-9	14.8	1
TACACTC	175	2.237357E-9	14.8	5
GTAGGAC	155	1.2102464E-7	14.32258	3
CGCTACA	105	1.6558598E-4	14.095238	2
CGTACAC	110	2.4566965E-4	13.454545	3
TAGATAG	125	4.84521E-5	13.32	5
GGGTTAG	125	4.84521E-5	13.32	1
TAAGACC	140	9.560043E-6	13.214285	4
ACCCTCG	155	1.8877854E-6	13.129032	7
TACACTG	1010	0.0	13.004951	5
>>END_MODULE