FastQCFastQC Report
Thu 9 Feb 2017
SRR2727202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643141
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT454680.8057214944655823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT271260.48068974353112925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190860.33821589784837913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT145570.25795917557261105No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62130.11009825910782665No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT61260.10855656450902078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC15750.019.9682543
AATCTCG13350.019.67790236
ATCTCCG14750.018.43728810
ACGAGAC15050.017.57807422
CACGAGA16200.016.55864121
CCCACGA16750.016.34626819
TTATACA18550.016.1563362
CTTATAC17700.016.0960451
TACACAT20050.015.5012475
CGAGACA17000.015.45294223
CCACGAG17800.015.07022420
GCCCACG19500.014.32564118
CGAGCCC19700.014.27411215
ACACATC21450.013.8857816
TCTCCGA19500.013.7564111
CCGAGCC21500.013.68139514
ATACACA23900.013.3912134
AAATCTC21250.013.14588335
CTCCGAG21950.012.97949812
TCCGAGC21550.012.70533713
Produced by FastQC (version 0.11.5)