##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727202.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5643141
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34739199321796	31.0	31.0	34.0	30.0	34.0
2	31.543620831023006	31.0	31.0	34.0	30.0	34.0
3	31.64292191175092	31.0	31.0	34.0	30.0	34.0
4	35.388597591305974	37.0	35.0	37.0	33.0	37.0
5	35.203112592791854	37.0	35.0	37.0	32.0	37.0
6	35.227394991548145	37.0	35.0	37.0	32.0	37.0
7	35.11386017113519	37.0	35.0	37.0	32.0	37.0
8	35.07608794463934	37.0	35.0	37.0	32.0	37.0
9	36.61574626613086	39.0	35.0	39.0	32.0	39.0
10	36.41809924650119	38.0	35.0	39.0	32.0	39.0
11	36.5115867209414	38.0	35.0	39.0	32.0	39.0
12	36.35736144108397	38.0	35.0	39.0	32.0	39.0
13	36.393992813576695	38.0	35.0	39.0	32.0	39.0
14	37.34532576804301	39.0	36.0	41.0	32.0	41.0
15	37.303496935483274	39.0	36.0	41.0	32.0	41.0
16	37.20847007012584	39.0	36.0	40.0	31.0	41.0
17	37.170089140072875	39.0	36.0	40.0	31.0	41.0
18	37.10171693388487	39.0	36.0	40.0	31.0	41.0
19	37.10325472994561	39.0	36.0	40.0	31.0	41.0
20	37.00206480752475	39.0	36.0	40.0	31.0	41.0
21	36.89858289913366	39.0	36.0	40.0	31.0	41.0
22	37.20221043564214	39.0	36.0	40.0	31.0	41.0
23	37.22353189473735	39.0	36.0	41.0	31.0	41.0
24	37.207798812753396	39.0	36.0	41.0	31.0	41.0
25	37.17261379788313	39.0	36.0	41.0	31.0	41.0
26	36.99576423839135	39.0	36.0	41.0	31.0	41.0
27	36.87417663319063	39.0	36.0	41.0	30.0	41.0
28	36.73123124160818	39.0	35.0	40.0	30.0	41.0
29	36.61152361778662	39.0	35.0	40.0	30.0	41.0
30	36.47284021434162	39.0	35.0	40.0	30.0	41.0
31	36.34635498209242	38.0	35.0	40.0	30.0	41.0
32	36.20944381861095	38.0	35.0	40.0	30.0	41.0
33	36.082934840720796	38.0	35.0	40.0	29.0	41.0
34	35.96528599941061	38.0	35.0	40.0	29.0	41.0
35	35.81917091917427	38.0	34.0	40.0	27.0	41.0
36	35.66778607871042	38.0	34.0	40.0	27.0	41.0
37	35.54774619312188	38.0	34.0	40.0	27.0	41.0
38	35.39631457020124	38.0	34.0	40.0	26.0	41.0
39	35.27853229256544	38.0	34.0	40.0	26.0	41.0
40	35.119253089724324	38.0	33.0	40.0	25.0	41.0
41	34.97687812514343	38.0	33.0	40.0	25.0	41.0
42	34.796425430447336	38.0	33.0	40.0	24.0	41.0
43	34.62583178410747	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	9.0
14	15.0
15	38.0
16	119.0
17	263.0
18	523.0
19	1142.0
20	2717.0
21	5128.0
22	9558.0
23	16143.0
24	25063.0
25	38058.0
26	54246.0
27	75659.0
28	100904.0
29	131866.0
30	166404.0
31	206270.0
32	251128.0
33	304345.0
34	371347.0
35	450692.0
36	563860.0
37	737121.0
38	1018114.0
39	1112404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.510116617677994	20.181313917196114	13.854500534365524	25.45406893076037
2	19.483635089039954	23.295661051176992	32.009460688648396	25.211243171134655
3	20.80293581181119	23.252316396134706	30.307181762780694	25.63756602927341
4	16.159511165856035	16.456881017149847	32.26752618798645	35.116081629007674
5	15.090213056877367	37.33729141270793	32.674409517678185	14.898086012736526
6	35.517896859213685	34.93423254885887	13.63830887798125	15.909561713946188
7	29.463006506482824	30.916859954411912	19.837090726600664	19.783042812504597
8	28.64215159607034	34.52525818511357	18.349727571932014	18.482862646884065
9	26.948396292065002	14.624142831093534	18.574956748378252	39.85250412846321
10	17.204726941963706	28.48551896895718	31.862592127327673	22.447161961751444
11	35.83683271426321	22.593693831148293	19.93418204507029	21.63529140951821
12	21.540149360081557	25.952408419353688	28.85665979283523	23.65078242772952
13	31.709432743218713	19.80148998580755	23.72554575545782	24.763531515515915
14	22.295774640399735	21.33085102782298	25.53149035262454	30.841883979152744
15	25.719807461837302	28.23032421128588	22.150270567402092	23.899597759474734
16	24.158318922032958	26.17109868422568	24.7532358308963	24.917346562845054
17	24.02598127532167	26.663625806975226	25.706711918061238	23.603680999641867
18	24.066720998110807	25.372429999533946	26.47153420408953	24.08931479826572
19	24.85312700852238	25.62968035000366	26.846254594737225	22.670938046736737
20	24.112794629799257	25.489687392181054	26.649342981151808	23.74817499686788
21	23.617751177934416	25.96617734697751	26.89452558424466	23.521545890843417
22	24.185998542301178	25.68488010489194	25.99298865649467	24.136132696312213
23	23.717642355560496	25.526652621297252	26.32641998489848	24.429285038243773
24	23.689874132154415	25.7587042393589	26.476318773534103	24.075102854952586
25	24.203187551046483	25.381272592692618	26.214018044206234	24.20152181205467
26	23.956020237665513	25.77475912793956	26.658274177448337	23.610946456946582
27	24.247418237467397	26.199363085203792	26.28954335892015	23.263675318408666
28	23.63697805885056	26.547874667671778	26.240262293641077	23.574884979836582
29	23.668024598357544	26.661038595349645	26.59490521324915	23.076031593043663
30	23.907766968785644	26.298173304547944	26.378873042513025	23.415186684153383
31	23.880353157931015	25.92031636281993	26.67907819421843	23.520252285030622
32	23.2906992754567	26.31699261102992	26.336857434538675	24.055450678974704
33	23.439836077106705	26.237834567663647	26.155734900120343	24.16659445510931
34	24.001260999858058	26.30377302286085	26.255572915863702	23.43939306141739
35	23.675041966876247	26.595135581407586	26.217827979134317	23.511994472581847
36	23.668875188481024	26.56072212266183	26.278095833508324	23.492306855348822
37	23.303085994129866	26.560828446427266	26.587037254606965	23.549048304835907
38	23.66113127423185	26.355162842821045	26.474404945756273	23.50930093719083
39	23.10528834916583	26.743368631051396	26.589978878784	23.56136414099878
40	23.834421291263148	26.360372707327357	26.787528434962017	23.01767756644748
41	23.589345011935727	26.334695517974833	26.398702424766636	23.677257045322808
42	23.37667976043838	26.57133677857775	26.48865233032455	23.563331130659325
43	23.580626463170066	26.10110220531438	26.358175349508368	23.960095982007186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1373.0
1	1407.0
2	1441.0
3	1773.0
4	2105.0
5	2105.0
6	3418.0
7	4731.0
8	4611.0
9	4491.0
10	7014.0
11	9537.0
12	9537.0
13	25019.0
14	40501.0
15	60672.0
16	80843.0
17	62037.5
18	43232.0
19	43232.0
20	52792.5
21	62353.0
22	49871.5
23	37390.0
24	44719.5
25	52049.0
26	52049.0
27	64799.5
28	77550.0
29	89484.0
30	101418.0
31	115279.0
32	129140.0
33	129140.0
34	145032.0
35	160924.0
36	184680.5
37	208437.0
38	233659.0
39	258881.0
40	258881.0
41	284609.0
42	310337.0
43	334593.0
44	358849.0
45	385779.5
46	412710.0
47	412710.0
48	444755.0
49	476800.0
50	495962.5
51	515125.0
52	508777.5
53	502430.0
54	502430.0
55	475904.0
56	449378.0
57	408908.5
58	368439.0
59	337931.0
60	307423.0
61	307423.0
62	268562.5
63	229702.0
64	193012.5
65	156323.0
66	130308.0
67	104293.0
68	104293.0
69	85440.0
70	66587.0
71	54343.5
72	42100.0
73	34273.5
74	26447.0
75	26447.0
76	21370.5
77	16294.0
78	13241.5
79	10189.0
80	8088.5
81	5988.0
82	5988.0
83	4676.0
84	3364.0
85	2664.5
86	1965.0
87	1548.5
88	1132.0
89	1132.0
90	822.0
91	512.0
92	361.0
93	210.0
94	158.5
95	107.0
96	107.0
97	65.5
98	24.0
99	20.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5643141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.58535265081664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.96587796061097	37.39414322796
2	11.818943615192396	11.484550870084805
3	4.010351741301499	5.845330608148498
4	1.9434493011977738	3.7769267863070803
5	1.1674460566649152	2.8360389181935086
6	0.7468204688476289	2.1770721507486166
7	0.5175349560520701	1.76012328442403
8	0.3841088721007428	1.492965200585761
9	0.29724819857840645	1.2997717697476663
>10	1.9411002839093514	17.650013615173076
>50	0.14512879571697979	4.786124189239058
>100	0.05718408577807343	4.784889117822769
>500	0.002730497386034568	0.8981219344111566
>1k	0.0018567280670193109	1.7165330701694779
>5k	7.281286536699636E-5	0.2182546221489426
>10k+	1.456257307339927E-4	1.8791406348356605
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	45468	0.8057214944655823	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27126	0.48068974353112925	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19086	0.33821589784837913	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14557	0.25795917557261105	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6213	0.11009825910782665	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	6126	0.10855656450902078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.316188271744406E-5
2	0.0	0.0	0.0	0.0	5.316188271744406E-5
3	0.0	0.0	0.0	0.0	5.316188271744406E-5
4	0.0	0.0	0.0	5.316188271744406E-5	5.316188271744406E-5
5	0.0	0.0	0.0	1.7720627572481354E-4	5.316188271744406E-5
6	1.7720627572481354E-5	0.0	0.0	1.7720627572481354E-4	1.2404439300736948E-4
7	1.7720627572481354E-5	0.0	0.0	1.7720627572481354E-4	1.594856481523322E-4
8	3.544125514496271E-5	0.0	0.0	1.7720627572481354E-4	1.594856481523322E-4
9	3.544125514496271E-5	0.0	0.0	1.949269032972949E-4	2.4808878601473896E-4
10	7.088251028992542E-5	0.0	0.0	2.1264753086977625E-4	2.4808878601473896E-4
11	7.088251028992542E-5	0.0	0.0	3.189712963046644E-4	2.4808878601473896E-4
12	7.088251028992542E-5	0.0	0.0	3.366919238771457E-4	2.4808878601473896E-4
13	7.088251028992542E-5	0.0	0.0	3.366919238771457E-4	2.658094135872203E-4
14	7.088251028992542E-5	0.0	0.0	3.898538065945898E-4	3.721331790221084E-4
15	7.088251028992542E-5	0.0	0.0	4.252950617395525E-4	3.721331790221084E-4
16	7.088251028992542E-5	0.0	0.0	4.607363168845152E-4	4.4301568931203383E-4
17	7.088251028992542E-5	0.0	0.0	4.7845694445699654E-4	4.4301568931203383E-4
18	7.088251028992542E-5	0.0	0.0	5.316188271744406E-4	4.607363168845152E-4
19	8.860313786240677E-5	0.0	0.0	5.847807098918847E-4	4.607363168845152E-4
20	8.860313786240677E-5	0.0	0.0	6.911044753267728E-4	4.7845694445699654E-4
21	8.860313786240677E-5	0.0	0.0	9.746345164864745E-4	5.316188271744406E-4
22	1.0632376543488813E-4	0.0	0.0	0.0013290470679361015	5.316188271744406E-4
23	1.2404439300736948E-4	0.0	0.0	0.0015416945988058778	7.088251028992542E-4
24	1.2404439300736948E-4	0.0	0.0	0.0020201515432628743	7.265457304717355E-4
25	1.4176502057985083E-4	0.0	0.0	0.002498608487719871	7.265457304717355E-4
26	1.4176502057985083E-4	0.0	0.0	0.003047947942466793	7.265457304717355E-4
27	1.4176502057985083E-4	0.0	0.0	0.004820010699714928	7.265457304717355E-4
28	1.7720627572481354E-4	0.0	0.0	0.009888110185444596	7.442663580442168E-4
29	1.7720627572481354E-4	0.0	0.0	0.021512841872992364	7.442663580442168E-4
30	1.7720627572481354E-4	1.7720627572481354E-5	0.0	0.036114638992716996	7.619869856166983E-4
31	2.1264753086977625E-4	1.7720627572481354E-5	0.0	0.06987243451829397	7.619869856166983E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	1575	0.0	19.968254	3
AATCTCG	1335	0.0	19.677902	36
ATCTCCG	1475	0.0	18.437288	10
ACGAGAC	1505	0.0	17.578074	22
CACGAGA	1620	0.0	16.558641	21
CCCACGA	1675	0.0	16.346268	19
TTATACA	1855	0.0	16.156336	2
CTTATAC	1770	0.0	16.096045	1
TACACAT	2005	0.0	15.501247	5
CGAGACA	1700	0.0	15.452942	23
CCACGAG	1780	0.0	15.070224	20
GCCCACG	1950	0.0	14.325641	18
CGAGCCC	1970	0.0	14.274112	15
ACACATC	2145	0.0	13.885781	6
TCTCCGA	1950	0.0	13.75641	11
CCGAGCC	2150	0.0	13.681395	14
ATACACA	2390	0.0	13.391213	4
AAATCTC	2125	0.0	13.145883	35
CTCCGAG	2195	0.0	12.979498	12
TCCGAGC	2155	0.0	12.705337	13
>>END_MODULE