FastQCFastQC Report
Thu 9 Feb 2017
SRR2727200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5939380
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG703551.184551249457014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC677261.140287370062195No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA432490.7281736477544795No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG404180.6805087399694918No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG318550.5363354424199159No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC290620.48931033205486096No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT284130.47838326559337846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT254160.4279234532897373No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG240510.40494125649478563No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC232810.39197694035404373No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT221670.37322077388548974No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG198250.3337890486885836No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC194300.3271385228761251No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA192330.3238216783570002No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT189230.31860227835228594No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT169620.28558536412891583No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT155500.2618118389461526No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT152820.2572995834582061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145040.24420057312379406No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC141850.23882964215120095No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT141440.238139334408642No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA136290.22946839569113275No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC135350.2278857389155097No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC134910.22714492085032448No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC127520.21470254471005393No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT126790.2134734601928147No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG114520.19281473823867137No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG107720.18136573177671744No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT106270.17892439951644784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC106120.1786718479033165No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT95680.16109425562937546No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC94830.15966312982163122No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT93360.1571881240129441No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT88730.14939269755429016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88680.1493085136832464No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG78960.13294316915233578No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT77810.13100694011832886No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG75520.12715131882452377No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT75400.12694927753401872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74830.12598958140411962No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG73770.12420488333799151No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC72450.12198242914243575No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72350.12181406140034819No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG71600.1205513033346915No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC71490.12036609881839519No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT71460.12031558849576891No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT69450.11693139687980901No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA68640.11556761816889978No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG68610.11551710784627352No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT67690.11396812461906798No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG63160.10634106590250161No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTC61170.1029905478349592No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT60090.10117217622041359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT24850.018.9094561
AAAAGTA27500.017.3563632
TGGGCTG54300.016.69429236
AAGACGG19900.016.0829145
TAATACG1059.356141E-615.8571424
GGGCTGT59100.015.68274137
CAAGACG20450.015.6503684
AGTACTC33950.014.7128115
TCTTATA61150.014.34014737
AAGTACT35900.014.0682444
AAAGTAC34550.014.0289433
TTAGAGT11100.013.8333334
TTATACT6350.013.6929134
TCTAGAT11350.013.6916292
TACGACG17750.013.5492965
TACACTC4650.013.5268815
GGTTATC12600.013.3611112
AGGTTAT12800.013.2968761
CGCAAGA24500.013.2897962
ACGACGG18150.013.2506896
Produced by FastQC (version 0.11.5)