##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5939380
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167836878596756	31.0	31.0	33.0	30.0	34.0
2	31.274471072738233	31.0	31.0	34.0	30.0	34.0
3	31.349617973593205	31.0	31.0	34.0	28.0	34.0
4	35.17613606133973	35.0	35.0	37.0	33.0	37.0
5	35.005716084843876	35.0	35.0	37.0	32.0	37.0
6	35.068953998565505	36.0	35.0	37.0	32.0	37.0
7	34.862788035114775	35.0	35.0	37.0	32.0	37.0
8	34.80094656344602	35.0	35.0	37.0	32.0	37.0
9	36.44714498819742	38.0	35.0	39.0	32.0	39.0
10	36.23377844152083	38.0	35.0	39.0	32.0	39.0
11	36.34842491977277	38.0	35.0	39.0	32.0	39.0
12	36.206267489199206	38.0	35.0	39.0	32.0	39.0
13	36.28638174354899	38.0	35.0	39.0	32.0	39.0
14	37.246904222326236	39.0	36.0	40.0	31.0	41.0
15	37.1216682549357	39.0	36.0	40.0	31.0	41.0
16	37.032263300209785	39.0	36.0	40.0	31.0	41.0
17	36.980233121975694	39.0	36.0	40.0	31.0	41.0
18	36.938181089608676	39.0	36.0	40.0	31.0	41.0
19	36.931723513228654	39.0	36.0	40.0	31.0	41.0
20	36.85410598412629	39.0	36.0	40.0	31.0	41.0
21	36.70572063077291	39.0	35.0	40.0	30.0	41.0
22	36.72002212352131	39.0	35.0	40.0	30.0	41.0
23	36.504392040920095	38.0	35.0	40.0	30.0	41.0
24	36.39276321770959	38.0	35.0	40.0	30.0	41.0
25	36.28807249241503	38.0	35.0	40.0	30.0	41.0
26	35.93394276843711	38.0	34.0	40.0	27.0	41.0
27	36.11121110284238	38.0	35.0	40.0	29.0	41.0
28	35.98026746899508	38.0	34.0	40.0	28.0	41.0
29	35.89496799329223	38.0	34.0	40.0	27.0	41.0
30	36.04044344695911	38.0	35.0	40.0	29.0	41.0
31	36.02102879425125	38.0	35.0	40.0	29.0	41.0
32	35.78891820358354	38.0	34.0	40.0	27.0	41.0
33	35.78396415114035	38.0	34.0	40.0	27.0	41.0
34	35.81224791139816	38.0	34.0	40.0	28.0	41.0
35	35.5681550936293	38.0	34.0	40.0	27.0	41.0
36	35.48451067283117	38.0	34.0	40.0	26.0	41.0
37	35.27549643228755	38.0	34.0	40.0	25.0	41.0
38	35.16706423902831	38.0	33.0	40.0	25.0	41.0
39	35.139796746461755	38.0	33.0	40.0	25.0	41.0
40	35.041472847334234	38.0	33.0	40.0	25.0	41.0
41	34.90909909788564	38.0	33.0	40.0	24.0	41.0
42	34.777417508224765	38.0	33.0	40.0	24.0	41.0
43	34.31269762163728	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	8.0
14	16.0
15	64.0
16	208.0
17	437.0
18	873.0
19	1715.0
20	3457.0
21	6637.0
22	11549.0
23	18836.0
24	29981.0
25	45080.0
26	64313.0
27	90217.0
28	122238.0
29	158547.0
30	200482.0
31	245474.0
32	294458.0
33	350013.0
34	418584.0
35	498139.0
36	604117.0
37	752768.0
38	1027344.0
39	993817.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.47421448029929	18.498732190902082	11.90206721913735	27.124986109661275
2	16.523020921375632	24.45098646660089	31.68527691442541	27.340715697598068
3	21.379807319955955	25.304274183500635	28.9764251487529	24.339493347790512
4	15.708878704511244	17.536426361000643	31.60068896080062	35.15400597368749
5	13.93542760355458	36.90878509204664	34.07062690045089	15.085160403947887
6	34.77549845270045	31.80087820614273	16.456532500025254	16.967090841131565
7	26.212146722385164	30.28933996477747	22.78315245025575	20.715360862581615
8	28.066515360189108	31.29427650697548	22.607511221710013	18.0316969111254
9	27.93172014587381	13.432479484390628	18.883351460926896	39.75244890880866
10	18.113809858941508	26.270856554051097	32.68115527209911	22.93417831490829
11	35.10549922719207	21.855042108772295	19.4646579272584	23.57480073677724
12	22.112964652876226	26.822850196485152	28.350349699800315	22.713835450838303
13	32.14311931548411	18.11480322861982	23.6535631665258	26.088514289370274
14	23.5324225760938	21.53275257686829	24.21729204058336	30.717532806454546
15	29.281608518060807	23.8239344847442	22.15569638581805	24.738760611376946
16	24.361903094262363	24.3465816297324	27.716866070195877	23.57464920580936
17	26.269206550178637	26.25469325081069	25.395445315841048	22.080654883169622
18	27.03172721731898	20.67256178254296	28.13751603702743	24.158194963110628
19	25.3974657287461	28.195687091918685	25.253191410551267	21.153655768783945
20	24.84870474696012	22.965915634291793	27.02645730699164	25.15892231175645
21	23.923035737736935	26.43878654000923	25.598328445056556	24.039849277197284
22	24.953092747054406	26.106394943579968	22.134212661927677	26.806299647437946
23	21.763551077721917	24.52862083247746	25.121308958174087	28.58651913162653
24	23.2072539558001	29.290161599358854	23.967737373261183	23.53484707157986
25	25.560277335344768	24.02499924234516	21.62749310534096	28.787230316969108
26	24.570561237031473	24.796679114655067	25.17821725499968	25.45454239331378
27	26.913364694631426	23.776606312443384	22.838427579983094	26.471601412942093
28	23.997252238449132	24.820873559193046	26.069842306772763	25.112031895585062
29	24.89374311796854	23.15910078156306	26.32114799861265	25.626008101855753
30	23.42448201664147	26.67731312022467	27.227235839431053	22.67096902370281
31	27.440153686074975	23.77015782792143	21.7571699402968	27.03251854570679
32	21.20830793786557	25.77444110328014	25.36130033774569	27.655950621108598
33	23.787432358259615	24.78022958625311	26.069151999030204	25.36318605645707
34	23.23321626163	24.66737268873182	25.848876482057047	26.25053456758113
35	23.342705804309542	26.545565362041156	27.53159420680273	22.580134626846572
36	25.581946263751437	24.84786290824968	24.527425421508642	25.04276540649024
37	23.68533415945772	25.14627789432567	26.21977378110173	24.948614165114876
38	24.62427054675741	24.328431587135356	26.56447305947759	24.48282480662965
39	25.133380925281763	21.052079509982523	25.852917307867152	27.961622256868562
40	24.20511905283043	23.68575507881294	27.03969101151972	25.06943485683691
41	22.742441130218978	23.08101182278285	27.63943374560981	26.53711330138836
42	25.196518828564596	24.04634827204186	27.20083577747172	23.55629712192182
43	23.850048321541976	20.89864598661813	28.56084305095818	26.690462640881705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	208.5
2	325.0
3	2180.5
4	4036.0
5	4036.0
6	5948.5
7	7861.0
8	8053.5
9	8246.0
10	9610.0
11	10974.0
12	10974.0
13	15914.0
14	20854.0
15	29445.5
16	38037.0
17	34678.0
18	31319.0
19	31319.0
20	32070.5
21	32822.0
22	29136.5
23	25451.0
24	29073.0
25	32695.0
26	32695.0
27	37251.5
28	41808.0
29	46552.5
30	51297.0
31	59585.0
32	67873.0
33	67873.0
34	83221.5
35	98570.0
36	124922.5
37	151275.0
38	178761.5
39	206248.0
40	206248.0
41	227304.0
42	248360.0
43	277975.5
44	307591.0
45	375065.0
46	442539.0
47	442539.0
48	720694.5
49	998850.0
50	995894.0
51	992938.0
52	717077.5
53	441217.0
54	441217.0
55	398190.0
56	355163.0
57	346683.0
58	338203.0
59	320999.5
60	303796.0
61	303796.0
62	253828.0
63	203860.0
64	180063.0
65	156266.0
66	133665.0
67	111064.0
68	111064.0
69	93678.5
70	76293.0
71	65835.0
72	55377.0
73	41773.0
74	28169.0
75	28169.0
76	22262.5
77	16356.0
78	14442.0
79	12528.0
80	10314.5
81	8101.0
82	8101.0
83	6930.5
84	5760.0
85	5104.0
86	4448.0
87	3015.5
88	1583.0
89	1583.0
90	1099.0
91	615.0
92	442.0
93	269.0
94	246.0
95	223.0
96	223.0
97	118.0
98	13.0
99	14.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5939380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.91561517156508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66829158025214	29.690825472870696
2	8.858578969617515	6.363226264794044
3	2.870365587434632	3.092728375200168
4	1.4308342788379245	2.0555717333210697
5	0.8513703759114727	1.5288745394853576
6	0.5474287616838578	1.1796744443090308
7	0.41291358299100106	1.0381031742071825
8	0.30911728834174507	0.8881710056767869
9	0.24324215693546086	0.7862572531795907
>10	1.5431863658023428	10.270029553163157
>50	0.11846572496613263	2.933668444192259
>100	0.10710589731577548	8.215352372890468
>500	0.019501916338627162	4.909675622125416
>1k	0.016777383569755503	11.773326117304936
>5k	0.001386165594082362	3.5393351126892645
>10k+	0.001433964407671409	11.735180514590661
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	70355	1.184551249457014	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	67726	1.140287370062195	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	43249	0.7281736477544795	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	40418	0.6805087399694918	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	31855	0.5363354424199159	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	29062	0.48931033205486096	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	28413	0.47838326559337846	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	25416	0.4279234532897373	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	24051	0.40494125649478563	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	23281	0.39197694035404373	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	22167	0.37322077388548974	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	19825	0.3337890486885836	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	19430	0.3271385228761251	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	19233	0.3238216783570002	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	18923	0.31860227835228594	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	16962	0.28558536412891583	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	15550	0.2618118389461526	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	15282	0.2572995834582061	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14504	0.24420057312379406	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	14185	0.23882964215120095	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	14144	0.238139334408642	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	13629	0.22946839569113275	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	13535	0.2278857389155097	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	13491	0.22714492085032448	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	12752	0.21470254471005393	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	12679	0.2134734601928147	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	11452	0.19281473823867137	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	10772	0.18136573177671744	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	10627	0.17892439951644784	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	10612	0.1786718479033165	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	9568	0.16109425562937546	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	9483	0.15966312982163122	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	9336	0.1571881240129441	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	8873	0.14939269755429016	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8868	0.1493085136832464	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	7896	0.13294316915233578	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	7781	0.13100694011832886	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	7552	0.12715131882452377	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	7540	0.12694927753401872	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7483	0.12598958140411962	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	7377	0.12420488333799151	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	7245	0.12198242914243575	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7235	0.12181406140034819	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	7160	0.1205513033346915	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	7149	0.12036609881839519	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	7146	0.12031558849576891	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	6945	0.11693139687980901	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	6864	0.11556761816889978	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	6861	0.11551710784627352	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	6769	0.11396812461906798	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6316	0.10634106590250161	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTC	6117	0.1029905478349592	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	6009	0.10117217622041359	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6836774208755796E-5
2	0.0	0.0	0.0	0.0	1.6836774208755796E-5
3	0.0	0.0	0.0	0.0	1.6836774208755796E-5
4	0.0	0.0	0.0	0.0	1.6836774208755796E-5
5	0.0	0.0	0.0	0.0	1.6836774208755796E-5
6	0.0	0.0	0.0	0.0	5.0510322626267386E-5
7	0.0	0.0	0.0	1.6836774208755796E-5	5.0510322626267386E-5
8	0.0	0.0	0.0	1.6836774208755796E-5	5.0510322626267386E-5
9	0.0	0.0	0.0	3.367354841751159E-5	8.418387104377898E-5
10	0.0	0.0	0.0	6.734709683502319E-5	8.418387104377898E-5
11	1.6836774208755796E-5	0.0	0.0	8.418387104377898E-5	1.0102064525253477E-4
12	1.6836774208755796E-5	0.0	0.0	1.5153096787880218E-4	1.0102064525253477E-4
13	1.6836774208755796E-5	0.0	0.0	2.6938838734009274E-4	1.0102064525253477E-4
14	3.367354841751159E-5	1.6836774208755796E-5	0.0	4.545929036364065E-4	1.3469419367004637E-4
15	5.0510322626267386E-5	3.367354841751159E-5	0.0	6.061238715152087E-4	1.3469419367004637E-4
16	5.0510322626267386E-5	3.367354841751159E-5	0.0	0.0014816361303705101	1.3469419367004637E-4
17	5.0510322626267386E-5	5.0510322626267386E-5	0.0	0.0026938838734009273	1.3469419367004637E-4
18	5.0510322626267386E-5	5.0510322626267386E-5	0.0	0.0037040903259262753	1.5153096787880218E-4
19	5.0510322626267386E-5	5.0510322626267386E-5	0.0	0.006465321296162225	1.5153096787880218E-4
20	5.0510322626267386E-5	5.0510322626267386E-5	0.0	0.009243389040606932	1.5153096787880218E-4
21	5.0510322626267386E-5	5.0510322626267386E-5	0.0	0.015119423239462705	1.5153096787880218E-4
22	5.0510322626267386E-5	5.0510322626267386E-5	0.0	0.02439648582848715	1.5153096787880218E-4
23	5.0510322626267386E-5	5.0510322626267386E-5	0.0	0.0364516161619563	1.5153096787880218E-4
24	5.0510322626267386E-5	6.734709683502319E-5	0.0	0.05335573746754712	1.5153096787880218E-4
25	5.0510322626267386E-5	6.734709683502319E-5	0.0	0.0652425000589287	1.5153096787880218E-4
26	5.0510322626267386E-5	6.734709683502319E-5	0.0	0.08756806265973889	1.5153096787880218E-4
27	5.0510322626267386E-5	6.734709683502319E-5	0.0	0.1304176530210224	1.5153096787880218E-4
28	5.0510322626267386E-5	6.734709683502319E-5	0.0	0.20778263051025528	1.5153096787880218E-4
29	5.0510322626267386E-5	8.418387104377898E-5	0.0	0.27627462799147384	1.5153096787880218E-4
30	5.0510322626267386E-5	8.418387104377898E-5	0.0	0.38719529647875706	1.5153096787880218E-4
31	5.0510322626267386E-5	8.418387104377898E-5	0.0	0.6314800534735949	1.6836774208755796E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAGT	2485	0.0	18.909456	1
AAAAGTA	2750	0.0	17.356363	2
TGGGCTG	5430	0.0	16.694292	36
AAGACGG	1990	0.0	16.082914	5
TAATACG	105	9.356141E-6	15.857142	4
GGGCTGT	5910	0.0	15.682741	37
CAAGACG	2045	0.0	15.650368	4
AGTACTC	3395	0.0	14.712811	5
TCTTATA	6115	0.0	14.340147	37
AAGTACT	3590	0.0	14.068244	4
AAAGTAC	3455	0.0	14.028943	3
TTAGAGT	1110	0.0	13.833333	4
TTATACT	635	0.0	13.692913	4
TCTAGAT	1135	0.0	13.691629	2
TACGACG	1775	0.0	13.549296	5
TACACTC	465	0.0	13.526881	5
GGTTATC	1260	0.0	13.361111	2
AGGTTAT	1280	0.0	13.296876	1
CGCAAGA	2450	0.0	13.289796	2
ACGACGG	1815	0.0	13.250689	6
>>END_MODULE