FastQCFastQC Report
Thu 9 Feb 2017
SRR2727199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3659830
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG490441.3400622433282419No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC451961.2349207476850017No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG286960.7840801348696524No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA286530.7829052169089822No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG217840.5952189036102771No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT191960.524505236582027No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC177510.485022528368804No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG165090.451086525876885No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC162260.4433539262752642No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC134830.3684050898539003No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132470.36195670290696563No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA131310.3587871567805062No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG130290.3560001420831022No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT126000.34427828614990313No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT110520.30198123956577216No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT107910.2948497607812385No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT101940.278537527699374No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC101130.2763243101455532No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT100750.2752860105524027No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA92610.2530445403201788No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT88920.24296210479721733No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC88610.24211507091859455No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC88430.24162324479552327No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC82950.2266498717153529No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG77310.21123931985911915No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG73080.1996814059669438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC71030.1940800528986319No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC66090.18058215818767537No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT64500.17623769410054566No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT60240.16459780918785846No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT57770.1578488618323802No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT50870.1389955271146474No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT50830.13888623242063158No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG49880.1362904834377553No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT49540.13536147853862066No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG49370.13489697608905332No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC48820.1333941740463355No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG48360.13213728506515332No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT48240.13180940098310578No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG47150.12883112057117407No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT46290.12648128464983346No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA45420.1241041250549889No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG45130.12331173852337404No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT43670.11932248219179578No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT43350.11844812463966906No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39740.10858427850473928No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT38510.10522346666375214No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT37570.10265504135437986No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA37530.102545746660364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT10800.019.870371
AAAAGTA11500.018.821742
ATTAGCG650.001580826117.07692316
ATACTAG1201.0430085E-716.9583341
GGGCTGT29750.016.66554637
TGGGCTG28750.016.6017436
AAGACGG10400.016.543275
TCGTTAC3300.016.25757623
TAGTACG700.002593944515.8571424
TTAGAGT5750.015.7652184
CAAGACG11150.015.5964134
TACTCCT32850.015.20548137
TACCTGT45050.015.15316437
ATTAGAG6700.014.9104483
TGTACCT42450.014.7738535
TAGAGTG6050.014.6776865
TATACTG2151.2732926E-1114.6279075
TACACAG6000.014.4916675
GTACTCC34350.014.4337736
ATGTACC43600.014.38417434
Produced by FastQC (version 0.11.5)