##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727199.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3659830
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.140966110447753	31.0	31.0	33.0	30.0	34.0
2	31.26326031536984	31.0	31.0	34.0	30.0	34.0
3	31.33758425937817	31.0	31.0	34.0	28.0	34.0
4	35.163619075202945	35.0	35.0	37.0	33.0	37.0
5	34.98862460824683	35.0	35.0	37.0	32.0	37.0
6	35.047133063557595	36.0	35.0	37.0	32.0	37.0
7	34.834546686594734	35.0	35.0	37.0	32.0	37.0
8	34.77781071798417	35.0	35.0	37.0	32.0	37.0
9	36.42027744458076	38.0	35.0	39.0	32.0	39.0
10	36.19801274922606	38.0	35.0	39.0	32.0	39.0
11	36.314850416549405	38.0	35.0	39.0	32.0	39.0
12	36.16325266474126	38.0	35.0	39.0	31.0	39.0
13	36.2478161553952	38.0	35.0	39.0	32.0	39.0
14	37.20992532440032	39.0	36.0	40.0	31.0	41.0
15	37.08775216335185	39.0	36.0	40.0	31.0	41.0
16	36.98883582024302	39.0	36.0	40.0	31.0	41.0
17	36.924926021153986	38.0	36.0	40.0	31.0	41.0
18	36.867584013465105	38.0	36.0	40.0	31.0	41.0
19	36.86381498594197	39.0	36.0	40.0	31.0	41.0
20	36.77666694901129	39.0	36.0	40.0	30.0	41.0
21	36.61881453510136	38.0	35.0	40.0	30.0	41.0
22	36.626606427074485	38.0	35.0	40.0	30.0	41.0
23	36.42007552263357	38.0	35.0	40.0	30.0	41.0
24	36.30342884778801	38.0	35.0	40.0	30.0	41.0
25	36.199904367142736	38.0	34.0	40.0	29.0	41.0
26	35.84848394597563	38.0	34.0	40.0	27.0	41.0
27	36.05309372293249	38.0	34.0	40.0	29.0	41.0
28	35.93394037427968	38.0	34.0	40.0	27.0	41.0
29	35.8651385446865	38.0	34.0	40.0	27.0	41.0
30	36.04333889825484	38.0	35.0	40.0	29.0	41.0
31	36.03753972179036	38.0	35.0	40.0	29.0	41.0
32	35.834896156378846	38.0	34.0	40.0	27.0	41.0
33	35.848505258440966	38.0	34.0	40.0	28.0	41.0
34	35.88780817688253	38.0	34.0	40.0	28.0	41.0
35	35.67217056529948	38.0	34.0	40.0	27.0	41.0
36	35.60128803796898	38.0	34.0	40.0	27.0	41.0
37	35.41421923969146	38.0	34.0	40.0	26.0	41.0
38	35.32389373276901	38.0	34.0	40.0	26.0	41.0
39	35.28857897771208	38.0	33.0	40.0	26.0	41.0
40	35.20936436938328	38.0	33.0	40.0	25.0	41.0
41	35.08785490036423	38.0	33.0	40.0	25.0	41.0
42	34.97823040960919	38.0	33.0	40.0	25.0	41.0
43	34.518223524043464	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	6.0
14	11.0
15	38.0
16	105.0
17	223.0
18	521.0
19	978.0
20	2015.0
21	3827.0
22	6947.0
23	11686.0
24	18217.0
25	27444.0
26	39523.0
27	55192.0
28	74082.0
29	96694.0
30	122831.0
31	152095.0
32	181800.0
33	216536.0
34	260127.0
35	308908.0
36	372535.0
37	460565.0
38	640905.0
39	606016.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.14231262107803	18.180680523412292	11.70707382583344	27.96993302967624
2	17.11680597186208	23.70287690958323	31.214892495006595	27.965424623548092
3	21.610429992649934	24.21470396165942	28.759013396797116	25.415852648893527
4	15.996972536975761	17.15254533680526	31.169753786378056	35.680728339840925
5	14.229813953107112	36.530248672752556	33.891000401658	15.348936972482328
6	36.01530672189692	31.36164794539637	15.70657653497567	16.916468797731042
7	26.93354609367102	29.992294724071883	21.96779631840823	21.10636286384887
8	28.403231844102052	31.293502703677493	21.870332775019605	18.432932677200853
9	28.411019091050676	13.017407912389373	17.798449654765385	40.773123341794566
10	18.209725588347002	25.8351344188118	32.390712136902536	23.564427855938664
11	36.359803597434855	21.096471694040435	18.627231319487517	23.916493389037196
12	22.565665618348394	26.08077424361241	27.70309003423656	23.650470103802633
13	33.05153518059582	17.620709158622123	22.66594350010793	26.66181216067413
14	23.896164575950248	20.775117969960352	23.223619676323764	32.105097777765636
15	30.013743807772492	23.342423008718985	21.036031728249675	25.60780145525885
16	25.253686646647523	23.846599432214063	26.631838090840287	24.267875830298127
17	27.03792252645615	25.57137899847807	24.286455928280823	23.10424254678496
18	27.809133211105436	20.43644103687876	26.828650511089315	24.92577524092649
19	26.555659689111245	27.434143115937076	24.021197705904374	21.988999489047306
20	25.85325001434493	22.086217119374396	25.6909473937314	26.36958547254927
21	24.81746419915679	25.74592262482137	24.592317129484158	24.844296046537682
22	25.99454073003391	25.188628980034593	21.04605951642563	27.77077077350587
23	22.825021927247988	23.514152296691375	24.264159810701592	29.39666596535905
24	24.289242942978227	28.11073191924215	22.99642333113833	24.603601806641294
25	26.655773628829753	22.87352144771752	20.603142768926425	29.8675621545263
26	25.54831781804073	23.846162253438003	24.23085225270026	26.374667675821005
27	28.09616840126454	22.783080088419407	21.763852419374672	27.356899090941383
28	24.957006199741517	23.998027230773015	24.747570242333662	26.297396327151805
29	25.943199547519967	22.20083992972351	25.241828172346803	26.61413235040972
30	24.671719724686668	25.641354926321714	25.920876106267233	23.76604924272439
31	28.563348570835256	22.822043646836054	20.6947317225117	27.919876059816986
32	22.41268037039972	24.789074902386176	24.151558952191767	28.646685775022334
33	24.95441045075864	23.755474981078358	24.857985206963164	26.432129361199834
34	24.417773503140854	23.84083413710473	24.716120694130602	27.025271665623812
35	24.32479104220688	25.782317757928645	26.412784200359035	23.48010699950544
36	26.781599145315493	24.01188033323952	23.313842446233842	25.892678075211144
37	24.59062306172691	24.507176562845814	25.20917638250957	25.693023992917702
38	25.9758513373572	23.6136377919193	25.22338469273163	25.187126177991875
39	26.19233680252908	20.481115243057737	24.562834885773384	28.763713068639802
40	25.557225335603018	23.040796976908766	25.677176262285407	25.72480142520281
41	24.15776142607717	22.483639950489504	25.913580685441673	27.445017937991658
42	26.48169450493602	23.36430927119566	25.91382659850321	24.240169625365112
43	25.457439279966554	19.964725137506388	26.979340570463656	27.598495012063402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26.0
1	52.0
2	78.0
3	391.5
4	705.0
5	705.0
6	1057.0
7	1409.0
8	1414.0
9	1419.0
10	1712.0
11	2005.0
12	2005.0
13	2934.5
14	3864.0
15	5396.5
16	6929.0
17	6263.0
18	5597.0
19	5597.0
20	5792.0
21	5987.0
22	5579.0
23	5171.0
24	6502.0
25	7833.0
26	7833.0
27	9746.0
28	11659.0
29	14372.5
30	17086.0
31	21648.5
32	26211.0
33	26211.0
34	35129.0
35	44047.0
36	59144.5
37	74242.0
38	92311.5
39	110381.0
40	110381.0
41	124536.0
42	138691.0
43	160645.0
44	182599.0
45	225317.5
46	268036.0
47	268036.0
48	448322.0
49	628608.0
50	635785.0
51	642962.0
52	473251.5
53	303541.0
54	303541.0
55	276228.5
56	248916.0
57	243035.0
58	237154.0
59	223785.0
60	210416.0
61	210416.0
62	177179.5
63	143943.0
64	126615.5
65	109288.0
66	93754.5
67	78221.0
68	78221.0
69	65374.0
70	52527.0
71	44997.5
72	37468.0
73	28655.0
74	19842.0
75	19842.0
76	15675.0
77	11508.0
78	9994.0
79	8480.0
80	6878.0
81	5276.0
82	5276.0
83	4376.0
84	3476.0
85	3074.5
86	2673.0
87	1808.0
88	943.0
89	943.0
90	646.5
91	350.0
92	245.5
93	141.0
94	127.5
95	114.0
96	114.0
97	59.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3659830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.920278806257976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.21014171872507	31.9964163639476
2	8.950743613891841	6.967308739520071
3	3.0827536826226627	3.599448984560776
4	1.522271923797189	2.3698899077250113
5	0.9329465306521423	1.8155269542156238
6	0.620323544236335	1.4485899175058554
7	0.4296867685343461	1.1706470181501751
8	0.33897872018278613	1.0554517039122036
9	0.2550244913220833	0.8933061874211642
>10	1.4177314002996826	9.918735925250106
>50	0.103291394069022	2.8025310185887236
>100	0.10305782746785129	8.589975456492443
>500	0.018058836290222535	4.95787765244073
>1k	0.012634047523177103	9.576940329024286
>5k	9.993031939236126E-4	2.8148645959703678
>10k+	0.0013561971917534744	10.022489245274857
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	49044	1.3400622433282419	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	45196	1.2349207476850017	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	28696	0.7840801348696524	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	28653	0.7829052169089822	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	21784	0.5952189036102771	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	19196	0.524505236582027	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	17751	0.485022528368804	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	16509	0.451086525876885	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	16226	0.4433539262752642	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	13483	0.3684050898539003	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	13247	0.36195670290696563	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	13131	0.3587871567805062	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	13029	0.3560001420831022	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	12600	0.34427828614990313	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	11052	0.30198123956577216	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	10791	0.2948497607812385	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	10194	0.278537527699374	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	10113	0.2763243101455532	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	10075	0.2752860105524027	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9261	0.2530445403201788	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	8892	0.24296210479721733	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	8861	0.24211507091859455	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	8843	0.24162324479552327	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	8295	0.2266498717153529	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	7731	0.21123931985911915	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	7308	0.1996814059669438	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7103	0.1940800528986319	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	6609	0.18058215818767537	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	6450	0.17623769410054566	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	6024	0.16459780918785846	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	5777	0.1578488618323802	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	5087	0.1389955271146474	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	5083	0.13888623242063158	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4988	0.1362904834377553	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	4954	0.13536147853862066	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	4937	0.13489697608905332	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	4882	0.1333941740463355	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	4836	0.13213728506515332	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	4824	0.13180940098310578	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	4715	0.12883112057117407	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	4629	0.12648128464983346	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	4542	0.1241041250549889	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	4513	0.12331173852337404	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	4367	0.11932248219179578	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	4335	0.11844812463966906	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3974	0.10858427850473928	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	3851	0.10522346666375214	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3757	0.10265504135437986	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	3753	0.102545746660364	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7323673503960565E-5
2	0.0	0.0	0.0	0.0	2.7323673503960565E-5
3	0.0	0.0	0.0	0.0	2.7323673503960565E-5
4	0.0	0.0	0.0	0.0	2.7323673503960565E-5
5	0.0	0.0	0.0	0.0	2.7323673503960565E-5
6	0.0	0.0	0.0	0.0	5.464734700792113E-5
7	2.7323673503960565E-5	0.0	0.0	0.0	5.464734700792113E-5
8	5.464734700792113E-5	0.0	0.0	0.0	8.19710205118817E-5
9	5.464734700792113E-5	0.0	0.0	0.0	1.0929469401584226E-4
10	5.464734700792113E-5	0.0	0.0	2.7323673503960565E-5	1.0929469401584226E-4
11	5.464734700792113E-5	0.0	0.0	5.464734700792113E-5	1.0929469401584226E-4
12	5.464734700792113E-5	0.0	0.0	8.19710205118817E-5	1.0929469401584226E-4
13	5.464734700792113E-5	0.0	0.0	1.3661836751980284E-4	1.0929469401584226E-4
14	8.19710205118817E-5	0.0	0.0	2.459130615356451E-4	2.1858938803168452E-4
15	8.19710205118817E-5	0.0	0.0	4.098551025594085E-4	3.0056040854356623E-4
16	8.19710205118817E-5	0.0	0.0	0.0010109759196465409	4.098551025594085E-4
17	8.19710205118817E-5	0.0	0.0	0.001584773063229713	4.098551025594085E-4
18	8.19710205118817E-5	0.0	0.0	0.0023771595948445692	4.098551025594085E-4
19	8.19710205118817E-5	0.0	0.0	0.003961932658074282	4.098551025594085E-4
20	8.19710205118817E-5	0.0	0.0	0.005874589803351522	4.098551025594085E-4
21	8.19710205118817E-5	0.0	0.0	0.009590609399890159	4.6450244956732964E-4
22	8.19710205118817E-5	0.0	0.0	0.014481546957099101	4.6450244956732964E-4
23	8.19710205118817E-5	0.0	0.0	0.020957257577537754	5.737971435831719E-4
24	8.19710205118817E-5	0.0	0.0	0.03235122942868931	6.011208170871325E-4
25	8.19710205118817E-5	0.0	0.0	0.04005650535680619	6.011208170871325E-4
26	8.19710205118817E-5	0.0	0.0	0.053827636802802316	6.011208170871325E-4
27	8.19710205118817E-5	0.0	0.0	0.07926597683498961	6.011208170871325E-4
28	8.19710205118817E-5	0.0	0.0	0.12716437648743248	6.011208170871325E-4
29	8.19710205118817E-5	0.0	0.0	0.16883297858097235	6.011208170871325E-4
30	8.19710205118817E-5	0.0	0.0	0.23588527335969156	6.011208170871325E-4
31	8.19710205118817E-5	0.0	0.0	0.4021225029577877	6.011208170871325E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAAGT	1080	0.0	19.87037	1
AAAAGTA	1150	0.0	18.82174	2
ATTAGCG	65	0.0015808261	17.076923	16
ATACTAG	120	1.0430085E-7	16.958334	1
GGGCTGT	2975	0.0	16.665546	37
TGGGCTG	2875	0.0	16.60174	36
AAGACGG	1040	0.0	16.54327	5
TCGTTAC	330	0.0	16.257576	23
TAGTACG	70	0.0025939445	15.857142	4
TTAGAGT	575	0.0	15.765218	4
CAAGACG	1115	0.0	15.596413	4
TACTCCT	3285	0.0	15.205481	37
TACCTGT	4505	0.0	15.153164	37
ATTAGAG	670	0.0	14.910448	3
TGTACCT	4245	0.0	14.77385	35
TAGAGTG	605	0.0	14.677686	5
TATACTG	215	1.2732926E-11	14.627907	5
TACACAG	600	0.0	14.491667	5
GTACTCC	3435	0.0	14.43377	36
ATGTACC	4360	0.0	14.384174	34
>>END_MODULE