FastQCFastQC Report
Thu 9 Feb 2017
SRR2727198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537933
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG482751.9021384725286286No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC445451.7551684776548475No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA287931.134505914852756No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG287591.133166241977231No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG217370.8564843910379036No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT196580.7745673349138846No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC174070.6858731101254446No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG163650.6448160767049407No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC151260.5959968210350707No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA134270.5290525794022143No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT129520.510336561288261No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC128560.5065539555220725No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG126320.4977278754009661No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT121840.4800757151587532No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT104590.41210701779755415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT103910.409427672046504No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT100370.395479313283684No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT99920.3937062168307832No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC97390.3837374745511406No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA92290.3636423814182644No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC85750.3378733796361054No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT84680.3336573502925412No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC83000.32703779020171136No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC82640.32561931303939073No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG77110.30382992774040923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC70270.27687886165631637No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG69920.2754997866373935No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC66530.2621424600255405No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT63360.24965198056843893No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT57320.22585308595616987No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT54960.21655418011428984No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG49050.1932675133661921No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT48520.1911791997661089No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG47980.18905148402262786No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT47460.18700257256594244No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT47180.18589931255080414No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT47060.18542648683003057No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG47030.18530828039983718No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG46860.18463844396207466No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC44000.17336943095030485No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA43930.17309361594652026No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG43740.17234497522196213No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT43250.1704142701954701No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT41510.1635582972442535No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT41120.16202161365173942No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT36410.14346320411137725No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA36020.14192652051886318No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC34790.1370800568809342No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG32400.12766294460886085No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT32190.12683549959750712No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30510.12021593950667729No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA30410.11982191807269932No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG29630.11674855088767118No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG28870.11375398798943864No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27210.10721323218540442No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCC27070.10666160217783525No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC26860.10583415716648155No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC26790.10555834216269695No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTG26450.10421866928717188No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG25750.10146051924932611No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC25740.10142111710592834No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG25660.10110589995874594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA10200.021.0392152
AAAAAGT10350.020.73431
GTACCGT1101.7571438E-920.1818186
GCCTAAT759.2730115E-619.7333321
TAATAGT1052.262641E-819.3809534
TACTTAC2500.018.531
CCCGTTA1306.9849193E-1018.522
CAAGACG4700.018.54
AAGACGG4600.017.695655
TGGGCTG24500.016.68775636
GGGCTGT25650.016.58869637
TACTCCT28050.016.29055237
ATAATAC1151.2435667E-616.0869563
GTACTCC28600.015.84790236
GACGGAC5400.015.4166677
ATAAGAC1101.4531635E-515.1363643
AAAGTAC14700.015.102043
TAGTACC1601.0981239E-815.031254
CGAACGT2109.094947E-1214.97619154
TACCTGT39200.014.81887837
Produced by FastQC (version 0.11.5)