##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727198.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2537933
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126832347426035	31.0	31.0	33.0	30.0	34.0
2	31.233062496133666	31.0	31.0	34.0	28.0	34.0
3	31.316578491236765	31.0	31.0	34.0	28.0	34.0
4	35.13007869002058	35.0	35.0	37.0	32.0	37.0
5	34.95650988422468	35.0	35.0	37.0	32.0	37.0
6	35.04609696158251	36.0	35.0	37.0	32.0	37.0
7	34.82831973893716	35.0	35.0	37.0	32.0	37.0
8	34.79436612392841	35.0	35.0	37.0	32.0	37.0
9	36.42089133164666	38.0	35.0	39.0	32.0	39.0
10	36.21502655901476	38.0	35.0	39.0	32.0	39.0
11	36.33181214791722	38.0	35.0	39.0	32.0	39.0
12	36.193777771123194	38.0	35.0	39.0	32.0	39.0
13	36.26282057091342	38.0	35.0	39.0	32.0	39.0
14	37.21425073081125	39.0	36.0	40.0	31.0	41.0
15	37.088013355750526	39.0	36.0	40.0	31.0	41.0
16	36.948038029372725	38.0	36.0	40.0	31.0	41.0
17	36.93829742550335	38.0	36.0	40.0	31.0	41.0
18	36.870086404960254	38.0	36.0	40.0	31.0	41.0
19	36.85732917299235	39.0	36.0	40.0	31.0	41.0
20	36.82158906480195	39.0	36.0	40.0	31.0	41.0
21	36.65631992649136	39.0	35.0	40.0	30.0	41.0
22	36.72687222239515	39.0	35.0	40.0	30.0	41.0
23	36.514597903096735	38.0	35.0	40.0	30.0	41.0
24	36.45692340971964	38.0	35.0	40.0	30.0	41.0
25	36.33834068905681	38.0	35.0	40.0	30.0	41.0
26	35.97692216461191	38.0	34.0	40.0	28.0	41.0
27	36.20721271995754	38.0	35.0	40.0	29.0	41.0
28	36.10214532850158	38.0	35.0	40.0	29.0	41.0
29	36.05912488627556	38.0	35.0	40.0	29.0	41.0
30	36.239363292884406	38.0	35.0	40.0	30.0	41.0
31	36.23702319958801	38.0	35.0	40.0	30.0	41.0
32	35.99130197684494	38.0	35.0	40.0	29.0	41.0
33	36.07316189986103	38.0	35.0	40.0	29.0	41.0
34	36.118692258621486	38.0	35.0	40.0	30.0	41.0
35	35.95772780447711	38.0	35.0	40.0	29.0	41.0
36	35.912361358633184	38.0	35.0	40.0	28.0	41.0
37	35.72597858178289	38.0	34.0	40.0	27.0	41.0
38	35.681016007908795	38.0	34.0	40.0	27.0	41.0
39	35.6538537463361	38.0	34.0	40.0	27.0	41.0
40	35.609912870040304	38.0	34.0	40.0	27.0	41.0
41	35.51463533513296	38.0	34.0	40.0	27.0	41.0
42	35.43487633440284	38.0	34.0	40.0	26.0	41.0
43	34.94327194610732	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	3.0
15	7.0
16	36.0
17	95.0
18	197.0
19	487.0
20	1006.0
21	2044.0
22	3823.0
23	6560.0
24	10775.0
25	16495.0
26	24431.0
27	34717.0
28	48211.0
29	63894.0
30	81973.0
31	102352.0
32	124299.0
33	149417.0
34	178879.0
35	214365.0
36	260698.0
37	323905.0
38	461739.0
39	427521.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.13397280385258	17.794638392739287	11.819342748606838	28.252046054801287
2	15.470030138699483	24.739226764457534	31.041126775214316	28.74961632162866
3	22.090654087401045	24.760503921892344	28.87018688042592	24.27865511028069
4	16.364537598116264	17.898738855596267	30.04618325227656	35.690540294010916
5	13.396531744533839	37.21634889494719	34.484637695321354	14.902481665197623
6	35.78979429322997	29.9507118588237	16.182736108478828	18.07675773946751
7	25.46012838006362	30.369556643142275	22.264496344072125	21.90581863272198
8	28.670181600538708	31.4008289422928	22.739646791306154	17.189342665862338
9	28.767623101161455	12.875596006671572	17.643609977095533	40.71317091507144
10	18.271995360003594	26.656692670767907	32.866194655256855	22.205117313971645
11	35.06944430763145	21.55876455367419	17.95981217786285	25.41197896083151
12	22.026980223670208	26.979159812335475	27.98880033476061	23.005059629233713
13	33.96783918251585	17.86449839298358	22.119614662798426	26.048047761702144
14	23.84909294295791	21.075733677760603	23.833174477025203	31.241998902256285
15	31.505244622296964	22.01579001494523	21.582090622565687	24.896874740192118
16	24.128887563225664	23.878605148362862	28.686494087905395	23.306013200506083
17	27.41995159052662	26.098600711681513	24.898175010924245	21.58327268686762
18	27.970675348797624	19.898712850181624	27.922762342425905	24.20784945859485
19	26.28792801070793	29.177523598928733	24.396428116896704	20.138120273466637
20	25.44153056837986	21.831112168839763	26.939127234643312	25.78823002813707
21	23.149350278356444	26.635848936910474	25.78787540884649	24.426925375886597
22	25.50800198429194	26.04655048025302	20.375084763860983	28.07036277159405
23	21.690446516909624	24.21671494085935	24.516250034969403	29.57658850726162
24	23.396559326034215	29.844838299513814	23.046865303378773	23.71173707107319
25	26.4493585922087	23.01096995074338	19.763051270463013	30.776620186584907
26	24.851089449563876	23.7830943527666	24.943960301552483	26.421855896117037
27	28.075721463096148	22.85556789718247	21.682408479656477	27.3863021600649
28	24.3786577502243	24.35072163055526	25.258428808010297	26.012191811210144
29	25.86746773851004	21.58811915050555	25.959550547630688	26.584862563353724
30	24.269750225872787	26.301718760897153	26.70271437425653	22.725816638973527
31	29.03354816695319	22.442081804365994	20.313381007300034	28.210989021380783
32	20.735023343799856	25.536962559689325	25.07832161053897	28.64969248597185
33	24.668736329918875	24.290357546869835	25.46903326447152	25.57187285873977
34	23.019244400856916	24.153001674985116	25.459734358629643	27.36801956552832
35	22.49369861221711	27.10977791769917	27.81267275377246	22.583850716311265
36	27.120534702846765	23.972027630359037	23.417324255604857	25.49011341118934
37	23.478161165011056	25.095067521483035	25.971410592793426	25.455360720712484
38	26.039300485867827	24.10749219936066	25.399961307095182	24.453246007676327
39	25.713720574971838	19.901943825940243	24.963149145387213	29.42118645370071
40	24.822168276309895	23.254120577651182	26.305619573093537	25.618091572945385
41	23.58446814789831	22.57372436545803	25.921094055674445	27.92071343096922
42	25.974996187842624	23.8385725706707	26.129058568528013	24.057372672958664
43	25.456385176440826	19.423207783657016	27.43374234071585	27.68666469918631
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	39.0
2	57.0
3	327.5
4	598.0
5	598.0
6	875.0
7	1152.0
8	1200.0
9	1248.0
10	1457.0
11	1666.0
12	1666.0
13	2396.0
14	3126.0
15	4297.5
16	5469.0
17	4800.5
18	4132.0
19	4132.0
20	4309.5
21	4487.0
22	4059.5
23	3632.0
24	4543.5
25	5455.0
26	5455.0
27	6629.5
28	7804.0
29	9472.0
30	11140.0
31	13880.0
32	16620.0
33	16620.0
34	21400.5
35	26181.0
36	34030.0
37	41879.0
38	51725.0
39	61571.0
40	61571.0
41	72046.5
42	82522.0
43	100920.0
44	119318.0
45	156370.0
46	193422.0
47	193422.0
48	361267.0
49	529112.0
50	535424.0
51	541736.0
52	375744.5
53	209753.0
54	209753.0
55	183529.0
56	157305.0
57	149222.5
58	141140.0
59	129774.0
60	118408.0
61	118408.0
62	100967.0
63	83526.0
64	72374.0
65	61222.0
66	51038.0
67	40854.0
68	40854.0
69	33281.5
70	25709.0
71	21208.5
72	16708.0
73	12710.0
74	8712.0
75	8712.0
76	6728.0
77	4744.0
78	3986.5
79	3229.0
80	2553.0
81	1877.0
82	1877.0
83	1492.0
84	1107.0
85	982.0
86	857.0
87	567.0
88	277.0
89	277.0
90	187.0
91	97.0
92	66.0
93	35.0
94	28.0
95	21.0
96	21.0
97	11.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2537933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.583578441765304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.36556847717691	31.77958372239747
2	8.76005863742075	6.759888191827745
3	3.0303036405463155	3.5075987465215714
4	1.5192210528649797	2.3446793865438886
5	0.9433582819329754	1.8199069134824952
6	0.6273212941500321	1.4522580216616496
7	0.4434681757752867	1.1977412402516636
8	0.34921918845618954	1.0779300760935207
9	0.2606867757186843	0.9052405793705464
>10	1.4580798229377527	10.281848546213974
>50	0.11700716426515807	3.153929427321494
>100	0.10428967125638211	8.061311115153407
>500	0.010293028797877912	2.7100320320483644
>1k	0.007901719077158802	7.132146737668169
>5k	0.001455579830002937	4.353076202346146
>10k+	0.001767489793574995	13.462829061097864
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	48275	1.9021384725286286	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	44545	1.7551684776548475	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	28793	1.134505914852756	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	28759	1.133166241977231	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	21737	0.8564843910379036	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	19658	0.7745673349138846	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	17407	0.6858731101254446	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	16365	0.6448160767049407	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	15126	0.5959968210350707	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	13427	0.5290525794022143	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	12952	0.510336561288261	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	12856	0.5065539555220725	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	12632	0.4977278754009661	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	12184	0.4800757151587532	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	10459	0.41210701779755415	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	10391	0.409427672046504	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	10037	0.395479313283684	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	9992	0.3937062168307832	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	9739	0.3837374745511406	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9229	0.3636423814182644	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	8575	0.3378733796361054	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	8468	0.3336573502925412	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	8300	0.32703779020171136	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	8264	0.32561931303939073	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	7711	0.30382992774040923	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7027	0.27687886165631637	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	6992	0.2754997866373935	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	6653	0.2621424600255405	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	6336	0.24965198056843893	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	5732	0.22585308595616987	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	5496	0.21655418011428984	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	4905	0.1932675133661921	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	4852	0.1911791997661089	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4798	0.18905148402262786	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	4746	0.18700257256594244	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	4718	0.18589931255080414	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	4706	0.18542648683003057	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	4703	0.18530828039983718	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	4686	0.18463844396207466	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	4400	0.17336943095030485	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	4393	0.17309361594652026	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	4374	0.17234497522196213	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	4325	0.1704142701954701	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	4151	0.1635582972442535	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	4112	0.16202161365173942	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	3641	0.14346320411137725	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	3602	0.14192652051886318	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	3479	0.1370800568809342	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	3240	0.12766294460886085	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	3219	0.12683549959750712	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3051	0.12021593950667729	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	3041	0.11982191807269932	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG	2963	0.11674855088767118	No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	2887	0.11375398798943864	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2721	0.10721323218540442	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCC	2707	0.10666160217783525	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	2686	0.10583415716648155	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	2679	0.10555834216269695	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTG	2645	0.10421866928717188	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2575	0.10146051924932611	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC	2574	0.10142111710592834	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	2566	0.10110589995874594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1820643019338966E-4
2	0.0	0.0	0.0	0.0	1.1820643019338966E-4
3	0.0	0.0	0.0	0.0	1.1820643019338966E-4
4	0.0	0.0	0.0	0.0	1.576085735911862E-4
5	0.0	0.0	0.0	0.0	1.576085735911862E-4
6	0.0	0.0	0.0	0.0	2.758150037845759E-4
7	0.0	0.0	0.0	0.0	3.152171471823724E-4
8	0.0	0.0	0.0	0.0	3.152171471823724E-4
9	0.0	0.0	0.0	0.0	3.54619290580169E-4
10	0.0	0.0	0.0	0.0	3.54619290580169E-4
11	0.0	0.0	0.0	0.0	3.54619290580169E-4
12	0.0	3.940214339779655E-5	0.0	3.940214339779655E-5	3.54619290580169E-4
13	0.0	3.940214339779655E-5	0.0	7.88042867955931E-5	3.54619290580169E-4
14	0.0	3.940214339779655E-5	0.0	1.9701071698898277E-4	3.54619290580169E-4
15	0.0	3.940214339779655E-5	0.0	3.54619290580169E-4	3.9402143397796553E-4
16	0.0	3.940214339779655E-5	0.0	0.0012608685887294897	5.516300075691518E-4
17	0.0	3.940214339779655E-5	0.0	0.002088313600083217	5.516300075691518E-4
18	0.0	3.940214339779655E-5	0.0	0.003191573615221521	6.698364377625414E-4
19	0.0	3.940214339779655E-5	0.0	0.005792115079476094	6.698364377625414E-4
20	0.0	3.940214339779655E-5	0.0	0.007407602958785752	6.698364377625414E-4
21	0.0	3.940214339779655E-5	0.0	0.01268749017409049	7.09238581160338E-4
22	0.0	3.940214339779655E-5	0.0	0.020410310280058616	7.09238581160338E-4
23	0.0	3.940214339779655E-5	0.0	0.029354596831358434	8.668471547515242E-4
24	0.0	3.940214339779655E-5	0.0	0.04511545419047706	8.668471547515242E-4
25	0.0	3.940214339779655E-5	0.0	0.0548871857531306	9.062492981493207E-4
26	0.0	3.940214339779655E-5	0.0	0.07360320386708397	9.062492981493207E-4
27	0.0	3.940214339779655E-5	0.0	0.10555834216269697	9.062492981493207E-4
28	0.0	3.940214339779655E-5	0.0	0.16994144447469653	9.062492981493207E-4
29	0.0	3.940214339779655E-5	0.0	0.2229373273447329	9.062492981493207E-4
30	0.0	3.940214339779655E-5	0.0	0.3145079086012121	9.062492981493207E-4
31	0.0	3.940214339779655E-5	0.0	0.5250729629190368	9.062492981493207E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	1020	0.0	21.039215	2
AAAAAGT	1035	0.0	20.7343	1
GTACCGT	110	1.7571438E-9	20.181818	6
GCCTAAT	75	9.2730115E-6	19.733332	1
TAATAGT	105	2.262641E-8	19.380953	4
TACTTAC	250	0.0	18.5	31
CCCGTTA	130	6.9849193E-10	18.5	22
CAAGACG	470	0.0	18.5	4
AAGACGG	460	0.0	17.69565	5
TGGGCTG	2450	0.0	16.687756	36
GGGCTGT	2565	0.0	16.588696	37
TACTCCT	2805	0.0	16.290552	37
ATAATAC	115	1.2435667E-6	16.086956	3
GTACTCC	2860	0.0	15.847902	36
GACGGAC	540	0.0	15.416667	7
ATAAGAC	110	1.4531635E-5	15.136364	3
AAAGTAC	1470	0.0	15.10204	3
TAGTACC	160	1.0981239E-8	15.03125	4
CGAACGT	210	9.094947E-12	14.9761915	4
TACCTGT	3920	0.0	14.818878	37
>>END_MODULE