FastQCFastQC Report
Thu 9 Feb 2017
SRR2727197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682495
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG219781.3062743128508554No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC173921.0337029233370678No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG120450.7159010873732166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA105630.6278176160998993No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG78800.46835206048160616No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT78670.4675793984528929No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC64270.38199221988772625No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC62290.37022398283501584No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG61960.36826260999289745No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC52970.31483005893033855No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA51150.30401279052835223No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51060.3034778706623199No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG48790.2899860029301721No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT46460.2761375219540029No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT42880.2548595983940517No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT42270.25123403041316617No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT40790.24243757039396846No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA39500.23477038564750563No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT38580.2293023159058422No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT36250.21545383492967288No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC34190.20321011355160043No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC32940.19578067096781862No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC32710.19441365353240278No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31070.18466622486248102No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC29630.17610750700596436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC26530.15768248939818544No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC25730.15292764614456505No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG25530.15173893533115995No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT24520.14573594572346427No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20560.12219947161804345No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG19820.1178012416084446No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG19640.11673140187638002No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT18770.11156050983806788No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT18750.11144163875672737No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG18570.11037179902466276No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA18430.1095397014552792No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT17640.1048442937423291No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT17260.10258574319685941No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG17130.10181308116814611No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16970.10086211251742205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC303.6001828E-430.8333323
CGCAATT303.6001828E-430.83333214
ATACTAA405.9417423E-527.7500021
TCTATAC358.8711124E-426.4285723
TAGTATA400.001931557223.1250027
ATTATAC603.72761E-521.5833323
TATTCTA450.003826535320.5555555
TTAGAAC555.1444565E-420.1818183
GTATACT759.269963E-619.7333344
AAGACGG3350.019.3283585
CGATCGT609.2392444E-418.525
AAAAAGT2850.018.175441
TAAGAGT1258.58563E-917.764
CAAGACG4000.017.5754
GCATAAA752.0679849E-417.2666661
TATACAA650.001580345417.0769235
AAAAGTA2950.016.9322032
TCAAAGC6400.016.7656253
CTTATAC6350.016.3149637
TCCTATT2200.015.9772732
Produced by FastQC (version 0.11.5)