##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1682495
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.129724011066898	31.0	31.0	33.0	30.0	34.0
2	31.257719636611103	31.0	31.0	34.0	30.0	34.0
3	31.32225236924924	31.0	31.0	34.0	28.0	34.0
4	35.15882602920068	35.0	35.0	37.0	33.0	37.0
5	34.98639936522842	35.0	35.0	37.0	32.0	37.0
6	35.022678224898144	35.0	35.0	37.0	32.0	37.0
7	34.812180125349556	35.0	35.0	37.0	32.0	37.0
8	34.74593980962796	35.0	35.0	37.0	31.0	37.0
9	36.385617193513205	38.0	35.0	39.0	32.0	39.0
10	36.16260850700893	38.0	35.0	39.0	31.0	39.0
11	36.27777199932243	38.0	35.0	39.0	32.0	39.0
12	36.10883657900915	38.0	35.0	39.0	31.0	39.0
13	36.20396137878567	38.0	35.0	39.0	32.0	39.0
14	37.151550524667236	39.0	36.0	40.0	31.0	41.0
15	37.04630147489294	39.0	36.0	40.0	31.0	41.0
16	36.942968626949856	38.0	36.0	40.0	31.0	41.0
17	36.847872356232855	38.0	36.0	40.0	31.0	41.0
18	36.79895036835176	38.0	36.0	40.0	31.0	41.0
19	36.78615211337924	39.0	35.0	40.0	30.0	41.0
20	36.67112888894172	38.0	35.0	40.0	30.0	41.0
21	36.509282939919586	38.0	35.0	40.0	30.0	41.0
22	36.508323650293164	38.0	35.0	40.0	30.0	41.0
23	36.28177854911902	38.0	35.0	40.0	29.0	41.0
24	36.15905901652011	38.0	34.0	40.0	29.0	41.0
25	36.027782549130904	38.0	34.0	40.0	29.0	41.0
26	35.680813910293935	38.0	34.0	40.0	27.0	41.0
27	35.9037780201427	38.0	34.0	40.0	27.0	41.0
28	35.7659481900392	38.0	34.0	40.0	27.0	41.0
29	35.67445074130978	38.0	34.0	40.0	27.0	41.0
30	35.85682869785646	38.0	34.0	40.0	27.0	41.0
31	35.85384503371481	38.0	34.0	40.0	27.0	41.0
32	35.662212369130366	38.0	34.0	40.0	27.0	41.0
33	35.666949381721786	38.0	34.0	40.0	27.0	41.0
34	35.699214559330045	38.0	34.0	40.0	27.0	41.0
35	35.462549368645966	38.0	34.0	40.0	26.0	41.0
36	35.37158565107177	38.0	34.0	40.0	26.0	41.0
37	35.18232089842763	38.0	33.0	40.0	25.0	41.0
38	35.08006205070446	38.0	33.0	40.0	25.0	41.0
39	35.04052374598439	38.0	33.0	40.0	25.0	41.0
40	34.96465843880665	38.0	33.0	40.0	25.0	41.0
41	34.83856237314227	38.0	33.0	40.0	24.0	41.0
42	34.71379172003483	38.0	33.0	40.0	24.0	41.0
43	34.268893518257116	37.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	7.0
15	17.0
16	38.0
17	103.0
18	224.0
19	525.0
20	1073.0
21	2035.0
22	3441.0
23	5790.0
24	9246.0
25	13945.0
26	19530.0
27	27179.0
28	36362.0
29	46825.0
30	58660.0
31	71836.0
32	85650.0
33	102296.0
34	121098.0
35	143650.0
36	171115.0
37	208444.0
38	286152.0
39	267248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.545386464744325	16.796067744629255	10.910463329757295	29.748082460869124
2	18.51316051459291	21.959233162654275	29.977622518937647	29.549983803815167
3	22.06574165153537	22.45118113278197	27.792237124033058	27.690840091649605
4	16.443555552913978	16.308636875592498	30.126449112775965	37.12135845871756
5	15.253418286532797	35.786852264048335	33.204972377332474	15.754757072086395
6	38.10382794599686	30.45922870498872	14.82892965506584	16.60801369394857
7	28.621719529627132	29.146119304960788	20.753761526780167	21.478399638631913
8	29.565258737767426	30.829928172149103	20.36029824754308	19.244514842540394
9	28.924603044882748	12.329784040962975	16.501861818311493	42.24375109584278
10	18.416637196544418	24.981173792492697	31.729485080193403	24.872703930769482
11	38.22151031652397	19.949301483808274	17.832326396215144	23.99686180345261
12	23.523695464176715	24.509552777274227	26.734938291049897	25.23181346749916
13	34.31059230488055	16.594759568379104	21.730584637695802	27.364063489044543
14	24.51745770418337	19.545674727116573	21.89950044428067	34.03736712441939
15	30.893286458503592	22.851241757033453	19.786269795749764	26.46920198871319
16	26.559246832828627	23.063664379388943	24.88887039783179	25.48821838995064
17	27.9817770632305	24.13671363064972	22.755669407635683	25.1258398984841
18	28.625047919904667	20.379436491638906	24.912525743018552	26.082989845437876
19	27.91621966187121	25.91585710507312	22.39525228901126	23.77267094404441
20	26.935176627567987	20.893613353977276	24.07448461956796	28.096725398886775
21	26.36180196672204	24.536952561523215	23.272045384978856	25.829200086775888
22	27.57291997895982	23.435017637496696	19.834888068018035	29.157174315525452
23	24.806968222788182	22.266098859134797	22.758344006965846	30.168588911111176
24	25.646019750430167	26.051429573341974	21.89325971251029	26.409290963717574
25	27.848284838885107	21.554655437311848	19.72909280562498	30.867966918178062
26	27.03841616171222	22.519769746715443	23.045120490699826	27.396693600872513
27	29.544396862992162	21.771535725217607	20.865619214321587	27.818448197468644
28	26.457612058282493	23.027111521876737	22.686070389510814	27.829206030329956
29	26.841862828715684	21.59418007185757	23.91983334274396	27.64412375668278
30	26.00394057634644	24.315792914689197	24.357694970861726	25.32257153810264
31	29.584278110781902	21.943779922080004	19.759048318122787	28.712893649015303
32	24.40025081798163	23.358464661113405	22.536827746887806	29.704456774017157
33	26.320375394874873	22.536173955940434	23.052609368824275	28.09084128036042
34	26.22296054371633	22.826873185358647	23.451600153343698	27.49856611758133
35	25.955203432996832	24.129165316984597	25.087801152455135	24.827830097563442
36	28.020826213450857	22.78253427201864	22.414866017432445	26.78177349709806
37	26.13814602717987	23.033233382565772	24.15026493392254	26.678355656331814
38	27.191819292182146	22.49011141192099	23.80013016383407	26.5179391320628
39	26.81143183189252	19.954710118009267	23.644706224981352	29.589151825116865
40	26.75009435392081	21.583422238996253	24.674426967093513	26.99205643998942
41	25.40227459814145	21.43519000056464	24.604174158021273	28.55836124327264
42	27.203706400316197	22.00297772058758	25.198589000264487	25.594726878831736
43	26.2627823559654	18.92350348738035	25.81267700646956	29.001037150184693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	11.0
2	17.0
3	73.5
4	130.0
5	130.0
6	194.5
7	259.0
8	264.0
9	269.0
10	319.0
11	369.0
12	369.0
13	552.0
14	735.0
15	997.0
16	1259.0
17	1144.5
18	1030.0
19	1030.0
20	1056.5
21	1083.0
22	1096.0
23	1109.0
24	1419.5
25	1730.0
26	1730.0
27	2190.0
28	2650.0
29	3283.0
30	3916.0
31	5090.0
32	6264.0
33	6264.0
34	8481.5
35	10699.0
36	14806.5
37	18914.0
38	24553.5
39	30193.0
40	30193.0
41	35429.5
42	40666.0
43	50286.0
44	59906.0
45	79767.5
46	99629.0
47	99629.0
48	171577.0
49	243525.0
50	259842.0
51	276159.0
52	216684.0
53	157209.0
54	157209.0
55	149326.0
56	141443.0
57	141496.0
58	141549.0
59	135488.0
60	129427.0
61	129427.0
62	112516.5
63	95606.0
64	85258.0
65	74910.0
66	63970.0
67	53030.0
68	53030.0
69	43407.5
70	33785.0
71	28746.5
72	23708.0
73	17916.0
74	12124.0
75	12124.0
76	9562.5
77	7001.0
78	6315.5
79	5630.0
80	4288.5
81	2947.0
82	2947.0
83	2370.0
84	1793.0
85	1490.5
86	1188.0
87	790.5
88	393.0
89	393.0
90	265.5
91	138.0
92	94.5
93	51.0
94	46.0
95	41.0
96	41.0
97	23.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1682495.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.71706539697125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.31234018928883	39.38825110394694
2	8.241990733597714	7.700832402054308
3	2.670957590836277	3.7433790133090534
4	1.3585390491926104	2.538678304218813
5	0.7870640080184714	1.8384660367100618
6	0.5171425523328295	1.4495629462133652
7	0.3460304204589715	1.131586804734626
8	0.2616303988184887	0.9778083561151205
9	0.19344656764810983	0.8133530356472733
>10	1.0830469924039827	9.240444787271054
>50	0.10760577074758972	3.510351283991263
>100	0.09928921153052826	9.64577429712476
>500	0.01071648614467327	3.4927977172280738
>1k	0.008650657490278423	7.7673219984611865
>5k	0.0010329143271974237	3.022977639524736
>10k+	5.164571635987118E-4	3.73841427344941
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	21978	1.3062743128508554	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	17392	1.0337029233370678	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	12045	0.7159010873732166	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	10563	0.6278176160998993	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	7880	0.46835206048160616	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	7867	0.4675793984528929	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	6427	0.38199221988772625	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	6229	0.37022398283501584	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	6196	0.36826260999289745	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	5297	0.31483005893033855	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	5115	0.30401279052835223	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	5106	0.3034778706623199	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	4879	0.2899860029301721	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	4646	0.2761375219540029	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	4288	0.2548595983940517	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	4227	0.25123403041316617	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	4079	0.24243757039396846	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	3950	0.23477038564750563	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	3858	0.2293023159058422	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	3625	0.21545383492967288	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	3419	0.20321011355160043	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	3294	0.19578067096781862	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	3271	0.19441365353240278	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	3107	0.18466622486248102	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	2963	0.17610750700596436	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	2653	0.15768248939818544	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	2573	0.15292764614456505	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	2553	0.15173893533115995	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	2452	0.14573594572346427	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	2056	0.12219947161804345	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	1982	0.1178012416084446	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1964	0.11673140187638002	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	1877	0.11156050983806788	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	1875	0.11144163875672737	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	1857	0.11037179902466276	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	1843	0.1095397014552792	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	1764	0.1048442937423291	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	1726	0.10258574319685941	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	1713	0.10181308116814611	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1697	0.10086211251742205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.943554067025459E-5	0.0	0.0	0.0
5	0.0	5.943554067025459E-5	0.0	0.0	0.0
6	0.0	5.943554067025459E-5	0.0	0.0	0.0
7	0.0	5.943554067025459E-5	0.0	0.0	0.0
8	0.0	5.943554067025459E-5	0.0	0.0	0.0
9	0.0	5.943554067025459E-5	0.0	0.0	0.0
10	0.0	5.943554067025459E-5	0.0	0.0	0.0
11	0.0	5.943554067025459E-5	0.0	0.0	0.0
12	0.0	5.943554067025459E-5	0.0	0.0	0.0
13	0.0	5.943554067025459E-5	0.0	0.0	0.0
14	0.0	5.943554067025459E-5	0.0	0.0	0.0
15	0.0	5.943554067025459E-5	0.0	5.943554067025459E-5	0.0
16	0.0	5.943554067025459E-5	0.0	2.97177703351273E-4	5.943554067025459E-5
17	0.0	5.943554067025459E-5	0.0	7.132264880430552E-4	5.943554067025459E-5
18	0.0	5.943554067025459E-5	0.0	8.915331100538188E-4	1.783066220107638E-4
19	0.0	5.943554067025459E-5	0.0	0.0013075818947456011	1.783066220107638E-4
20	0.0	5.943554067025459E-5	0.0	0.0018425017607778924	1.783066220107638E-4
21	0.0	5.943554067025459E-5	0.0	0.0034472613588747664	1.783066220107638E-4
22	0.0	5.943554067025459E-5	0.0	0.007072829339760296	1.783066220107638E-4
23	0.0	5.943554067025459E-5	0.0	0.01087670394265659	3.566132440215276E-4
24	0.0	5.943554067025459E-5	0.0	0.017295742335044086	3.566132440215276E-4
25	0.0	5.943554067025459E-5	0.0	0.021159052478610635	3.566132440215276E-4
26	0.0	5.943554067025459E-5	0.0	0.02989607695713806	3.566132440215276E-4
27	0.0	5.943554067025459E-5	0.0	0.04707294821084164	3.566132440215276E-4
28	0.0	5.943554067025459E-5	0.0	0.07875209138808734	3.566132440215276E-4
29	0.0	5.943554067025459E-5	0.0	0.11685027295772053	3.566132440215276E-4
30	0.0	5.943554067025459E-5	0.0	0.17462161848920799	3.566132440215276E-4
31	0.0	5.943554067025459E-5	0.0	0.3316503169400206	3.566132440215276E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	30	3.6001828E-4	30.833332	3
CGCAATT	30	3.6001828E-4	30.833332	14
ATACTAA	40	5.9417423E-5	27.750002	1
TCTATAC	35	8.8711124E-4	26.428572	3
TAGTATA	40	0.0019315572	23.125002	7
ATTATAC	60	3.72761E-5	21.583332	3
TATTCTA	45	0.0038265353	20.555555	5
TTAGAAC	55	5.1444565E-4	20.181818	3
GTATACT	75	9.269963E-6	19.733334	4
AAGACGG	335	0.0	19.328358	5
CGATCGT	60	9.2392444E-4	18.5	25
AAAAAGT	285	0.0	18.17544	1
TAAGAGT	125	8.58563E-9	17.76	4
CAAGACG	400	0.0	17.575	4
GCATAAA	75	2.0679849E-4	17.266666	1
TATACAA	65	0.0015803454	17.076923	5
AAAAGTA	295	0.0	16.932203	2
TCAAAGC	640	0.0	16.765625	3
CTTATAC	635	0.0	16.31496	37
TCCTATT	220	0.0	15.977273	2
>>END_MODULE