FastQCFastQC Report
Thu 9 Feb 2017
SRR2727196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5938725
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG585030.9851104403723021No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC491890.8282754294903367No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG347960.5859170108061915No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA307620.5179899725951277No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG216380.36435430163881977No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT184600.3108411317244021No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG159030.26778475177752803No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT153340.2582035706317433No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT149020.25092928195866954No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC144100.24264467541433554No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT130870.22036716635304718No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC120190.2023835082446148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT117730.1982412049724478No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT117190.19733191888831356No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC107390.18083006032439622No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT104440.17586266412403334No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC104320.1756606005497813No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC101990.17173719948305402No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT94260.15872093757498454No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA93870.15806423095866537No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG90330.15210335551822993No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA89160.15013323566927245No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT83010.1397774774888549No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC78710.1325368660781565No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG78670.13246951155340583No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG76640.12905126942230866No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC73450.12367974607344169No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC73170.1232082644001869No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT71610.12058143793491026No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG66870.11259992675195434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65020.10948477998223524No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT64990.10943426408867223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64160.10803665770009556No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT64020.10780091686346817No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT63580.10706001709121066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC31650.017.94470837
CAAGACG18600.015.118284
AAGACGG18750.014.85
TCTTATA50550.014.49258237
TACCGTC13950.014.3225817
ACGGACC19850.014.1662478
ACCGTCG13200.014.1553038
ATACCGT14750.014.0474586
ATTACGC800.006302698513.8753
TATACTG5900.013.796615
TACTTAC7450.013.65771831
TGGGCTG42450.013.59717436
TACTCCT36800.013.5733737
CGTATAC2051.4279067E-913.5365853
GGGCTGT46200.013.41450237
CTCTTAT79600.013.41017637
CGCGCTA10000.013.3224
ACGAACG8700.013.183907515
GTACTCC38050.013.12746536
CGCAAGA21150.013.0330962
Produced by FastQC (version 0.11.5)