##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727196.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5938725
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.154519362321036	31.0	31.0	33.0	30.0	34.0
2	31.298790565314945	31.0	31.0	34.0	30.0	34.0
3	31.362878227228908	31.0	31.0	34.0	28.0	34.0
4	35.197588708013924	35.0	35.0	37.0	33.0	37.0
5	35.03026946019558	35.0	35.0	37.0	32.0	37.0
6	35.063940829050004	36.0	35.0	37.0	32.0	37.0
7	34.87322463996902	36.0	35.0	37.0	32.0	37.0
8	34.80082458776926	36.0	35.0	37.0	32.0	37.0
9	36.44141444501977	38.0	35.0	39.0	32.0	39.0
10	36.22113012473216	38.0	35.0	39.0	32.0	39.0
11	36.337714576782055	38.0	35.0	39.0	32.0	39.0
12	36.1781306593587	38.0	35.0	39.0	31.0	39.0
13	36.28013150970958	38.0	35.0	39.0	32.0	39.0
14	37.25562860715053	39.0	36.0	40.0	31.0	41.0
15	37.164625403600944	39.0	36.0	40.0	31.0	41.0
16	37.09515173711529	39.0	36.0	40.0	31.0	41.0
17	37.00000606190723	39.0	36.0	40.0	31.0	41.0
18	36.979665668977766	39.0	36.0	40.0	31.0	41.0
19	36.97805741131303	39.0	36.0	40.0	31.0	41.0
20	36.87140067943877	39.0	36.0	40.0	31.0	41.0
21	36.73197984415847	39.0	35.0	40.0	30.0	41.0
22	36.716030966242755	39.0	35.0	40.0	30.0	41.0
23	36.51202202492959	38.0	35.0	40.0	30.0	41.0
24	36.40707559282506	38.0	35.0	40.0	30.0	41.0
25	36.27943691617308	38.0	35.0	40.0	29.0	41.0
26	35.96615132035917	38.0	34.0	40.0	28.0	41.0
27	36.15669053542638	38.0	35.0	40.0	29.0	41.0
28	36.043133500877715	38.0	34.0	40.0	29.0	41.0
29	35.96631027703758	38.0	34.0	40.0	28.0	41.0
30	36.13269851693756	38.0	35.0	40.0	29.0	41.0
31	36.13938884188104	38.0	35.0	40.0	29.0	41.0
32	35.964805913727275	38.0	34.0	40.0	28.0	41.0
33	35.94744275917811	38.0	34.0	40.0	28.0	41.0
34	35.985949004205445	38.0	35.0	40.0	29.0	41.0
35	35.74525474744158	38.0	34.0	40.0	27.0	41.0
36	35.66394268129944	38.0	34.0	40.0	27.0	41.0
37	35.48190933239037	38.0	34.0	40.0	26.0	41.0
38	35.40182732825649	38.0	34.0	40.0	26.0	41.0
39	35.371405983607595	38.0	34.0	40.0	26.0	41.0
40	35.281865046790344	38.0	33.0	40.0	26.0	41.0
41	35.158206012233265	38.0	33.0	40.0	25.0	41.0
42	35.041075651760266	38.0	33.0	40.0	25.0	41.0
43	34.613564022580604	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	6.0
13	10.0
14	13.0
15	59.0
16	146.0
17	319.0
18	627.0
19	1356.0
20	2836.0
21	5542.0
22	10000.0
23	17231.0
24	28134.0
25	42565.0
26	61864.0
27	86507.0
28	116886.0
29	154353.0
30	195870.0
31	241881.0
32	291517.0
33	348095.0
34	417470.0
35	498163.0
36	602023.0
37	746733.0
38	1030731.0
39	1037787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25366976918446	19.089939338831147	12.300670598487049	27.355720293497342
2	17.560334920374324	22.77557893318852	33.170520608379746	26.49356553805741
3	20.772876332882902	23.731979507385844	29.424935486994265	26.070208672736996
4	15.63738007737351	16.692589739380086	32.61940904823847	35.05062113500794
5	14.429292482814072	36.80524018202561	34.131130840373984	14.634336494786337
6	35.66066790430606	33.207144631212934	15.616112886183481	15.51607457829753
7	27.904120834017405	30.389216540587416	21.77846254877941	19.92820007661577
8	28.012241684873434	32.76380031067275	20.894282863746007	18.329675140707813
9	28.053159558659473	13.231308066967237	18.361096026504008	40.35443634786928
10	17.52147135959318	26.450795414840726	33.09861965320839	22.929113572357704
11	36.680819536179904	21.30726713225482	19.57477404661775	22.437139284947527
12	22.124614290104358	25.531338797469154	28.878538743585537	23.465508168840955
13	32.08783030027489	18.42129413299993	23.42332739771584	26.067548169009342
14	23.08411317244021	20.8911508783451	24.152086516887042	31.87264943232765
15	28.20719935676429	25.314996063969957	21.32157996876434	25.15622461050141
16	24.75472428846259	24.978795953676926	25.8257285865232	24.440751171337283
17	25.66973550720062	26.073205949088397	24.84920584805661	23.40785269565437
18	26.257235349338455	22.751701754164404	26.748350866558056	24.24271202993909
19	25.318447983363434	26.934922900117446	25.300969484190627	22.44565963232849
20	25.241091311687274	23.636975950225008	25.48705319744558	25.634879540642142
21	24.44034704418878	25.897360123595554	25.318195403895615	24.34409742832005
22	25.257997297399694	25.309001511267148	23.010006356583272	26.422994834749886
23	23.23811929328265	24.306412571722046	25.084559396166682	27.37090873882862
24	24.298649962744527	27.190213387553726	24.133294604481602	24.377842045220145
25	25.548598394436517	23.726843724873607	22.72635961422696	27.99819826646292
26	24.906305646414005	25.020808338490163	25.205443929462973	24.86744208563286
27	26.687243473978	23.925118607108427	23.287911125704593	26.099726793208983
28	24.302573363811256	24.99327717649832	25.537114447966523	25.167035011723897
29	25.4284379222813	23.66001119768974	25.508572968103422	25.40297791192554
30	24.088082879742707	26.103616517013332	25.943750552517585	23.864550050726375
31	26.724052721754248	24.093858530240077	22.760643067325056	26.421445680680616
32	22.86803985704002	25.407810599076402	24.870136266622886	26.85401327726069
33	24.43672673848343	24.624561669381894	25.366236018674044	25.572475573460636
34	24.632155892047535	24.551044205616527	25.06918572589234	25.747614176443594
35	24.042046062075613	25.8231354373203	26.803733124534308	23.33108537606978
36	25.619337484055922	24.67159196628906	24.752838361769573	24.956232187885448
37	24.040294844432097	24.66249910544772	26.11666645618378	25.18053959393641
38	24.92528278376251	24.11515939869248	26.277879511174536	24.681678306370475
39	24.981725875503578	21.945215513430913	25.811853554424562	27.261205056640947
40	24.44575224480002	23.665180657464354	27.24266572370332	24.646401374032305
41	23.613772316448394	23.11159381853849	27.00466851049678	26.269965354516334
42	24.62762630025805	23.93737713061305	27.05565588573305	24.37934068339585
43	24.18946827812367	21.667310744309596	27.804840264534896	26.33838071303184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	315.0
1	354.5
2	394.0
3	918.5
4	1443.0
5	1443.0
6	2357.5
7	3272.0
8	3354.0
9	3436.0
10	4585.5
11	5735.0
12	5735.0
13	9375.0
14	13015.0
15	19577.5
16	26140.0
17	23292.0
18	20444.0
19	20444.0
20	22167.0
21	23890.0
22	24238.5
23	24587.0
24	30093.5
25	35600.0
26	35600.0
27	42447.5
28	49295.0
29	56719.0
30	64143.0
31	75464.0
32	86785.0
33	86785.0
34	105329.0
35	123873.0
36	150943.0
37	178013.0
38	205232.0
39	232451.0
40	232451.0
41	260017.5
42	287584.0
43	313308.5
44	339033.0
45	385752.5
46	432472.0
47	432472.0
48	603393.5
49	774315.0
50	806360.5
51	838406.0
52	660891.5
53	483377.0
54	483377.0
55	453724.5
56	424072.0
57	408271.0
58	392470.0
59	367176.5
60	341883.0
61	341883.0
62	289142.0
63	236401.0
64	205505.5
65	174610.0
66	146227.0
67	117844.0
68	117844.0
69	98085.0
70	78326.0
71	66107.0
72	53888.0
73	41081.0
74	28274.0
75	28274.0
76	22249.0
77	16224.0
78	13874.5
79	11525.0
80	9028.5
81	6532.0
82	6532.0
83	5301.5
84	4071.0
85	3454.5
86	2838.0
87	1897.0
88	956.0
89	956.0
90	687.5
91	419.0
92	302.0
93	185.0
94	170.5
95	156.0
96	156.0
97	88.5
98	21.0
99	16.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5938725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.22115419691618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.26004503104646	36.29451872200388
2	10.060977467018297	9.09938026815467
3	3.467106250476723	4.703596391096993
4	1.7451630636040556	3.1567315199200654
5	1.005310016105542	2.2730639627006504
6	0.6689023197695392	1.814912096698396
7	0.48523906629189867	1.5360149447407507
8	0.35723056170077466	1.2923502651617367
9	0.2654798019868448	1.0804772755632492
>10	1.5195523543960117	12.31128248843136
>50	0.08773035652183096	2.728673286315902
>100	0.05820579847559648	5.462594243567126
>500	0.00969811468799267	3.0721795579315936
>1k	0.007705857724109269	5.951506718425263
>5k	9.773282967119783E-4	3.030037840013371
>10k+	6.766118977236773E-4	6.192680419274918
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	58503	0.9851104403723021	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	49189	0.8282754294903367	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	34796	0.5859170108061915	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	30762	0.5179899725951277	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	21638	0.36435430163881977	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	18460	0.3108411317244021	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	15903	0.26778475177752803	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	15334	0.2582035706317433	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	14902	0.25092928195866954	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	14410	0.24264467541433554	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	13087	0.22036716635304718	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	12019	0.2023835082446148	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11773	0.1982412049724478	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	11719	0.19733191888831356	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	10739	0.18083006032439622	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	10444	0.17586266412403334	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	10432	0.1756606005497813	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	10199	0.17173719948305402	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	9426	0.15872093757498454	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	9387	0.15806423095866537	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	9033	0.15210335551822993	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	8916	0.15013323566927245	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	8301	0.1397774774888549	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	7871	0.1325368660781565	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	7867	0.13246951155340583	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	7664	0.12905126942230866	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	7345	0.12367974607344169	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7317	0.1232082644001869	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	7161	0.12058143793491026	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	6687	0.11259992675195434	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6502	0.10948477998223524	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	6499	0.10943426408867223	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6416	0.10803665770009556	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	6402	0.10780091686346817	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6358	0.10706001709121066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0515893563012266E-5
2	0.0	0.0	0.0	0.0	5.0515893563012266E-5
3	0.0	0.0	0.0	0.0	5.0515893563012266E-5
4	0.0	0.0	0.0	0.0	5.0515893563012266E-5
5	0.0	0.0	0.0	0.0	5.0515893563012266E-5
6	0.0	0.0	0.0	0.0	6.735452475068302E-5
7	0.0	0.0	1.6838631187670754E-5	1.6838631187670754E-5	1.0103178712602453E-4
8	0.0	0.0	1.6838631187670754E-5	1.6838631187670754E-5	1.1787041831369528E-4
9	0.0	0.0	1.6838631187670754E-5	3.367726237534151E-5	1.3470904950136603E-4
10	0.0	0.0	1.6838631187670754E-5	5.0515893563012266E-5	1.3470904950136603E-4
11	0.0	0.0	1.6838631187670754E-5	6.735452475068302E-5	1.3470904950136603E-4
12	0.0	0.0	1.6838631187670754E-5	8.419315593835377E-5	1.515476806890368E-4
13	0.0	0.0	1.6838631187670754E-5	1.0103178712602453E-4	1.515476806890368E-4
14	0.0	1.6838631187670754E-5	1.6838631187670754E-5	1.515476806890368E-4	1.6838631187670753E-4
15	0.0	1.6838631187670754E-5	1.6838631187670754E-5	2.5257946781506133E-4	1.852249430643783E-4
16	0.0	1.6838631187670754E-5	1.6838631187670754E-5	4.5464304206711034E-4	2.3574083662739056E-4
17	0.0	1.6838631187670754E-5	1.6838631187670754E-5	6.903838786945009E-4	2.3574083662739056E-4
18	0.0	1.6838631187670754E-5	1.6838631187670754E-5	8.419315593835377E-4	2.5257946781506133E-4
19	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.0013639291262013311	2.5257946781506133E-4
20	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.0017848949058930999	2.5257946781506133E-4
21	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.002946760457842382	2.6941809900273207E-4
22	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.004664300838984799	2.6941809900273207E-4
23	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.006583904794379265	3.3677262375341507E-4
24	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.009985308294288757	3.5361125494108586E-4
25	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.012443748447688687	3.5361125494108586E-4
26	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.018118367157933732	3.5361125494108586E-4
27	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.040917873786039935	3.8728851731642733E-4
28	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.0771546081019074	3.8728851731642733E-4
29	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.11749996842756652	3.8728851731642733E-4
30	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.1829854051164181	3.8728851731642733E-4
31	0.0	1.6838631187670754E-5	1.6838631187670754E-5	0.41914720752350043	3.8728851731642733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3165	0.0	17.944708	37
CAAGACG	1860	0.0	15.11828	4
AAGACGG	1875	0.0	14.8	5
TCTTATA	5055	0.0	14.492582	37
TACCGTC	1395	0.0	14.322581	7
ACGGACC	1985	0.0	14.166247	8
ACCGTCG	1320	0.0	14.155303	8
ATACCGT	1475	0.0	14.047458	6
ATTACGC	80	0.0063026985	13.875	3
TATACTG	590	0.0	13.79661	5
TACTTAC	745	0.0	13.657718	31
TGGGCTG	4245	0.0	13.597174	36
TACTCCT	3680	0.0	13.57337	37
CGTATAC	205	1.4279067E-9	13.536585	3
GGGCTGT	4620	0.0	13.414502	37
CTCTTAT	7960	0.0	13.410176	37
CGCGCTA	1000	0.0	13.32	24
ACGAACG	870	0.0	13.1839075	15
GTACTCC	3805	0.0	13.127465	36
CGCAAGA	2115	0.0	13.033096	2
>>END_MODULE