##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727195.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3154207
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13082844594537	31.0	31.0	33.0	30.0	34.0
2	31.25003844072377	31.0	31.0	34.0	30.0	34.0
3	31.32521232753589	31.0	31.0	34.0	28.0	34.0
4	35.155576663167636	35.0	35.0	37.0	33.0	37.0
5	34.97550192488952	35.0	35.0	37.0	32.0	37.0
6	35.04816107503407	36.0	35.0	37.0	32.0	37.0
7	34.82173712758865	35.0	35.0	37.0	32.0	37.0
8	34.77643445721856	35.0	35.0	37.0	32.0	37.0
9	36.41563315280196	38.0	35.0	39.0	32.0	39.0
10	36.20934834016918	38.0	35.0	39.0	32.0	39.0
11	36.31658480245589	38.0	35.0	39.0	32.0	39.0
12	36.16904026907555	38.0	35.0	39.0	31.0	39.0
13	36.24064273524217	38.0	35.0	39.0	32.0	39.0
14	37.19600267198697	39.0	36.0	40.0	31.0	41.0
15	37.07232657843952	39.0	36.0	40.0	31.0	41.0
16	36.957654966842696	38.0	36.0	40.0	31.0	41.0
17	36.90843150116654	38.0	36.0	40.0	31.0	41.0
18	36.85412751921481	38.0	36.0	40.0	31.0	41.0
19	36.843216377365216	39.0	36.0	40.0	31.0	41.0
20	36.77913624565541	39.0	36.0	40.0	30.0	41.0
21	36.616015689521966	38.0	35.0	40.0	30.0	41.0
22	36.64873484841039	38.0	35.0	40.0	30.0	41.0
23	36.43177318419495	38.0	35.0	40.0	30.0	41.0
24	36.32692432678007	38.0	35.0	40.0	30.0	41.0
25	36.237489486263904	38.0	34.0	40.0	29.0	41.0
26	35.881837495129524	38.0	34.0	40.0	27.0	41.0
27	36.097955841198754	38.0	34.0	40.0	29.0	41.0
28	35.98078217441024	38.0	34.0	40.0	28.0	41.0
29	35.927955584398866	38.0	34.0	40.0	28.0	41.0
30	36.10467195082631	38.0	35.0	40.0	29.0	41.0
31	36.11914436814071	38.0	35.0	40.0	29.0	41.0
32	35.8916355204335	38.0	34.0	40.0	28.0	41.0
33	35.94329478058986	38.0	34.0	40.0	29.0	41.0
34	35.98102724393168	38.0	35.0	40.0	29.0	41.0
35	35.787754893702285	38.0	34.0	40.0	27.0	41.0
36	35.7376900121013	38.0	34.0	40.0	27.0	41.0
37	35.529743926127864	38.0	34.0	40.0	26.0	41.0
38	35.45716054780171	38.0	34.0	40.0	26.0	41.0
39	35.41034085587915	38.0	34.0	40.0	26.0	41.0
40	35.35838992177749	38.0	34.0	40.0	26.0	41.0
41	35.251590019298035	38.0	33.0	40.0	26.0	41.0
42	35.15474031983316	38.0	33.0	40.0	26.0	41.0
43	34.6803310626094	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	3.0
15	28.0
16	76.0
17	162.0
18	375.0
19	743.0
20	1574.0
21	3026.0
22	5379.0
23	9126.0
24	14605.0
25	22225.0
26	32451.0
27	45546.0
28	62543.0
29	82005.0
30	105197.0
31	129464.0
32	157043.0
33	187683.0
34	223868.0
35	268315.0
36	323778.0
37	399716.0
38	559634.0
39	519638.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.29081984790472	17.545646179848056	11.559387193040914	28.604146779206314
2	16.68225959805428	23.782047278444313	30.355141561730097	29.180551561771313
3	22.32500910688487	23.78166683416783	28.37118806723845	25.52213599170885
4	16.459319252033872	17.381706400372583	29.79997825126886	36.35899609632469
5	14.246845562133368	36.176160917783776	34.09259443023239	15.484399089850475
6	36.72514200875212	29.896547690116726	15.606394887843441	17.771915413287715
7	26.57289137967166	29.53512562745565	21.847805169413423	22.044177823459272
8	29.036046144086296	30.680611640263304	21.932834465207897	18.350507750442503
9	28.453871289994602	12.723705197534594	17.475422507146803	41.347001005324
10	18.619323335469105	25.93678854938817	32.23092206694107	23.212966048201654
11	36.06139356104403	20.91543135881697	17.847116565272984	25.176058514866018
12	22.747777809129204	26.30645357137309	27.403401235239162	23.542367384258547
13	34.139325668860664	17.23682053841108	21.943613719708313	26.680240073019938
14	24.236868410982538	20.642621108887273	23.02566064941204	32.09484983071815
15	31.34787919752889	22.062597667179105	20.865624862287095	25.72389827300491
16	25.167498518645097	23.223079525218225	27.316469718062258	24.29295223807442
17	27.71501680137036	25.465069350236053	24.09822817589334	22.721685672500254
18	28.367447031853015	19.488067840823383	26.94785091783767	25.196634209485936
19	26.808418090505793	27.945027070195454	23.658973555001307	21.587581284297446
20	26.247072560551672	21.29752422716708	25.853629771286414	26.601773440994837
21	24.589952403250642	25.71511635095604	24.510566364224033	25.184364881569284
22	26.287177728031164	25.18163836425447	20.220423073057667	28.3107608346567
23	22.859977167002672	23.13446771248685	23.945701724712425	30.059853395798058
24	24.523279543796587	28.55028221039393	22.43112135633457	24.495316889474914
25	26.953113730329047	22.468848747086035	19.69081293650036	30.887224586084557
26	25.784293801896958	23.328621108253202	24.094772473715263	26.792312616134577
27	28.647073575069744	22.271873722935748	21.231295219368924	27.849757482625588
28	25.214578497860156	23.46757838023947	24.740101077703525	26.577742044196846
29	26.416084930380286	21.16551006322667	25.284072985698145	27.134332020694902
30	24.535453760644117	25.619529726489098	26.16359674555284	23.68141976731394
31	29.28593462635775	22.083426991316678	19.861030046537845	28.769608335787726
32	21.91346351079685	24.61531535501633	24.28229979833283	29.18892133585399
33	25.39281664139354	23.552829601861895	24.7284024162016	26.325951340542964
34	24.251388700868397	23.471953489419054	24.528003393562948	27.748654416149606
35	23.758332918543392	26.00193963173628	26.979903348131558	23.259824101588766
36	27.516615111183256	23.582884699704238	22.810138966783093	26.090361222329417
37	24.434572619996086	24.358737394216675	25.180687253563256	26.02600273222398
38	26.340249704600872	23.562055375566665	24.94687254197331	25.150822377859157
39	26.348968219270326	19.60695667722505	24.343297697329312	29.70077740617531
40	25.469285940967097	22.874973012234136	25.757472480404743	25.898268566394027
41	24.28917949899927	22.080129807587138	25.379501091716556	28.251189601697035
42	26.57935893237191	23.302972823280147	25.76124521947989	24.356423024868057
43	26.02929991595352	19.168082500609504	26.87287169168035	27.92974589175663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	37.0
2	53.0
3	280.5
4	508.0
5	508.0
6	749.0
7	990.0
8	1046.5
9	1103.0
10	1286.5
11	1470.0
12	1470.0
13	2222.5
14	2975.0
15	4195.5
16	5416.0
17	4796.5
18	4177.0
19	4177.0
20	4189.0
21	4201.0
22	3718.0
23	3235.0
24	3957.0
25	4679.0
26	4679.0
27	5620.0
28	6561.0
29	8120.0
30	9679.0
31	12366.5
32	15054.0
33	15054.0
34	20153.5
35	25253.0
36	34703.0
37	44153.0
38	55897.5
39	67642.0
40	67642.0
41	80323.0
42	93004.0
43	113493.5
44	133983.0
45	177689.5
46	221396.0
47	221396.0
48	408727.0
49	596058.0
50	608786.0
51	621514.0
52	442069.0
53	262624.0
54	262624.0
55	237943.5
56	213263.0
57	209656.0
58	206049.0
59	196659.0
60	187269.0
61	187269.0
62	159876.5
63	132484.0
64	116493.0
65	100502.0
66	85264.5
67	70027.0
68	70027.0
69	57782.5
70	45538.0
71	38266.0
72	30994.0
73	23666.5
74	16339.0
75	16339.0
76	12971.5
77	9604.0
78	8331.0
79	7058.0
80	5513.5
81	3969.0
82	3969.0
83	3270.0
84	2571.0
85	2203.5
86	1836.0
87	1209.5
88	583.0
89	583.0
90	391.0
91	199.0
92	152.0
93	105.0
94	83.5
95	62.0
96	62.0
97	33.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3154207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.943623577542745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.19020116625319	32.39727879558622
2	8.567949704418572	6.6733401624039095
3	2.9068535574352827	3.3961023220740203
4	1.403214308973105	2.185849993890815
5	0.8358284616197238	1.6275094492357578
6	0.5782385373474	1.3511222361891626
7	0.4065043768447945	1.1081527404127083
8	0.31120298365709087	0.9695497481399947
9	0.22693495948670214	0.7953902674951536
>10	1.3312162891034727	9.322166924606998
>50	0.11070563538443945	3.013875593619238
>100	0.10353418130083783	8.690857776258852
>500	0.014595758315020866	3.8477920711599363
>1k	0.009537001171746504	6.751794618180304
>5k	0.0017415393444058834	4.4356460532420865
>10k+	0.0017415393444058834	13.433571247504759
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	56111	1.7789257331557502	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	49709	1.5759587116508207	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	32791	1.039595689185903	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	31635	1.0029462238844817	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	24131	0.7650417363223149	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	22229	0.7047413184993883	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	18532	0.5875327776521959	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	18408	0.5836015201285141	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	16995	0.5388042065723652	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	15253	0.4835763791025763	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	14843	0.47057786632266047	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	14351	0.4549796509867615	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	13803	0.4376060290272642	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	12097	0.3835195343869315	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	11650	0.3693479850878525	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	11520	0.365226505425928	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	10701	0.3392611835558034	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	10497	0.3327936308555526	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	10454	0.3314303721981468	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	10137	0.3213803025609923	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	10103	0.32030237711095055	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	9516	0.30169231125287593	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	9480	0.3005509784234199	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	8947	0.2836529118095293	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	8805	0.2791509878711194	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	8095	0.25664136817907	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7948	0.2519809257921246	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	7460	0.23650952521505406	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	7414	0.23505115548852692	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	6527	0.20692998271831875	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	5711	0.1810597719173155	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	5636	0.17868199518928213	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	5434	0.17227784986844555	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	5414	0.17164377607430328	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	5227	0.1657151860990734	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	5192	0.16460555695932447	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	5183	0.16432022375196048	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	5168	0.1638446684063538	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	5108	0.1619424470239271	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	5061	0.16045237360769285	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	5016	0.1590257075708728	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	5001	0.15855015222526614	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	4968	0.15750393046493144	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	4154	0.13169712704334244	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	4037	0.12798779534761034	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	4023	0.1275439436917108	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	3914	0.12408824151363561	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	3755	0.11904735485020483	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	3687	0.11689150395012121	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	3537	0.11213595049405443	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3510	0.11127995087196244	No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	3243	0.10281506572016358	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.17036897071118E-5	0.0	0.0	0.0
6	0.0	3.17036897071118E-5	0.0	0.0	3.17036897071118E-5
7	0.0	3.17036897071118E-5	0.0	0.0	3.17036897071118E-5
8	0.0	3.17036897071118E-5	0.0	0.0	3.17036897071118E-5
9	0.0	3.17036897071118E-5	0.0	0.0	9.511106912133541E-5
10	0.0	3.17036897071118E-5	0.0	0.0	9.511106912133541E-5
11	0.0	3.17036897071118E-5	0.0	0.0	9.511106912133541E-5
12	0.0	3.17036897071118E-5	0.0	0.0	9.511106912133541E-5
13	0.0	3.17036897071118E-5	0.0	6.34073794142236E-5	9.511106912133541E-5
14	0.0	6.34073794142236E-5	0.0	2.2192582794978261E-4	9.511106912133541E-5
15	0.0	6.34073794142236E-5	0.0	2.8533320736400626E-4	9.511106912133541E-5
16	0.0	6.34073794142236E-5	0.0	8.559996220920187E-4	1.268147588284472E-4
17	0.0	6.34073794142236E-5	0.0	0.0015217771059413666	1.5851844853555903E-4
18	0.0	6.34073794142236E-5	0.0	0.0023460730383262733	1.5851844853555903E-4
19	0.0	6.34073794142236E-5	0.0	0.004406812869288541	1.5851844853555903E-4
20	0.0	6.34073794142236E-5	0.0	0.005738367836987237	1.5851844853555903E-4
21	0.0	6.34073794142236E-5	0.0	0.010271995465104225	1.9022213824267082E-4
22	0.0	6.34073794142236E-5	0.0	0.015439696887363448	1.9022213824267082E-4
23	0.0	6.34073794142236E-5	0.0	0.022699841830292052	2.536295176568944E-4
24	0.0	6.34073794142236E-5	0.0	0.035032577126358544	2.536295176568944E-4
25	0.0	6.34073794142236E-5	0.0	0.04314872169137916	2.8533320736400626E-4
26	0.0	6.34073794142236E-5	0.0	0.060332121512633764	2.8533320736400626E-4
27	0.0	6.34073794142236E-5	0.0	0.08854840535196326	2.8533320736400626E-4
28	0.0	6.34073794142236E-5	0.0	0.14453712137472272	2.8533320736400626E-4
29	0.0	9.511106912133541E-5	0.0	0.19206095224568331	2.8533320736400626E-4
30	0.0	9.511106912133541E-5	0.0	0.27249321303262597	2.8533320736400626E-4
31	0.0	9.511106912133541E-5	0.0	0.449463208977724	3.1703689707111805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	915	0.0	20.420767	2
AAAAAGT	910	0.0	20.329672	1
TATACTA	70	1.2200536E-4	18.5	5
CTATACT	125	8.594725E-9	17.76	4
GGGCTGT	2760	0.0	16.422102	37
CGTTAAT	80	3.384906E-4	16.1875	2
GTACTCC	3055	0.0	16.047462	36
TACTCCT	3020	0.0	15.988409	37
TACCTGT	3845	0.0	15.733419	37
TGGGCTG	2605	0.0	15.6238	36
GCGTTAA	95	7.065921E-5	15.578948	1
TATTAAG	95	7.065921E-5	15.578948	2
TTAGAGT	310	0.0	15.5161295	4
GTATAGA	155	7.223207E-9	15.5161295	1
ATTACAC	180	2.0190782E-10	15.416666	3
TAGAGTG	325	0.0	15.369231	5
GTATTAC	85	5.3678366E-4	15.235294	1
TATACTG	135	3.978239E-7	15.074073	5
ATTAGAG	320	0.0	15.03125	3
AAAGTAC	1455	0.0	15.003436	1
>>END_MODULE