FastQCFastQC Report
Thu 9 Feb 2017
SRR2727194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022713
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG872931.449396642343741No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC791341.3139261326249483No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA496060.8236487443449488No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG491350.8158283484535955No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG367820.6107214472945997No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT320710.532500884568134No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC305270.5068645973998761No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC286040.47493546513008345No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG280300.46540487650665074No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT252320.41894740792064966No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG237160.39377602751451046No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC229260.38065901529759094No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT226290.3757276828565465No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT221600.3679404945910589No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA217180.36060160927475704No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT184410.3061909142939403No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC173140.28747841711866395No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT171890.2854029405020628No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT169630.28165047877924787No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA158780.2636353417471495No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC153100.2542043760013137No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC152940.25393871499438875No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT149630.2484428529136288No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC141630.2351598025673812No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG132470.21995070992092766No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG126870.21065257467855433No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT125970.20915823151460144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC121860.20233406439921675No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC115700.1921061156326061No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT108910.18083212665122844No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT103600.17201550198390658No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT95770.15901471645751675No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG92680.1538841382612786No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT92140.15298753236290688No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG86160.14305845222908678No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT86140.14302524460322116No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT85050.14121542899354494No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG84790.1407837298572919No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC84700.1406342955408966No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT83950.1393890095709359No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG83060.13791127021991584No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG82550.13706447576034256No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT78490.1303233277096219No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA78320.13004106288976414No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT76990.12783275576970046No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT67420.11194290679300177No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC65680.1090538433426929No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC64030.10631421420877933No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA62760.10420552996631254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA22800.018.7434222
AAAAAGT22550.018.623061
AAGACGG13550.016.5202965
TGGGCTG56250.016.51022136
GGGCTGT59950.016.38615437
TCGTTAC5900.016.30508423
CAAGACG14100.016.1382964
TAAACGG1152.2128115E-514.4782615
TACTCCT57300.014.43193737
AAAGTAC30300.014.4092423
TCTTATA50000.014.39337
GACGGAC15500.014.0838727
AAGTACT31750.014.04252054
AGTACTC31750.013.9842535
ACGGACC15550.013.9196158
TACCTGT84950.013.850537
GTACTCC60350.013.76387836
CTTATAC30650.013.701467537
TAACACG950.00124614713.6315794
TTAGAGT7750.013.6064524
Produced by FastQC (version 0.11.5)