##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727194.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6022713
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1542811354285	31.0	31.0	33.0	30.0	34.0
2	31.267918295293168	31.0	31.0	34.0	30.0	34.0
3	31.34634972644388	31.0	31.0	34.0	28.0	34.0
4	35.17355002637516	35.0	35.0	37.0	33.0	37.0
5	34.99699803062175	35.0	35.0	37.0	32.0	37.0
6	35.06493983691403	36.0	35.0	37.0	32.0	37.0
7	34.85429838679014	35.0	35.0	37.0	32.0	37.0
8	34.800484432846126	35.0	35.0	37.0	32.0	37.0
9	36.445918475610576	38.0	35.0	39.0	32.0	39.0
10	36.232138572766125	38.0	35.0	39.0	32.0	39.0
11	36.348713113176736	38.0	35.0	39.0	32.0	39.0
12	36.19610995908322	38.0	35.0	39.0	32.0	39.0
13	36.27709406043422	38.0	35.0	39.0	32.0	39.0
14	37.24316516493481	39.0	36.0	40.0	31.0	41.0
15	37.129368442427854	39.0	36.0	40.0	31.0	41.0
16	37.01912261135472	39.0	36.0	40.0	31.0	41.0
17	36.96665124172445	39.0	36.0	40.0	31.0	41.0
18	36.91319925090237	38.0	36.0	40.0	31.0	41.0
19	36.8987497826976	39.0	36.0	40.0	31.0	41.0
20	36.83058930418899	39.0	36.0	40.0	31.0	41.0
21	36.680625990313665	39.0	35.0	40.0	30.0	41.0
22	36.71214700086157	39.0	35.0	40.0	30.0	41.0
23	36.50243171142307	38.0	35.0	40.0	30.0	41.0
24	36.41859125613324	38.0	35.0	40.0	30.0	41.0
25	36.304242954960664	38.0	35.0	40.0	30.0	41.0
26	35.95672730877264	38.0	34.0	40.0	28.0	41.0
27	36.15917477721419	38.0	35.0	40.0	29.0	41.0
28	36.047610105279794	38.0	34.0	40.0	29.0	41.0
29	35.984953458682156	38.0	34.0	40.0	28.0	41.0
30	36.15637985738321	38.0	35.0	40.0	29.0	41.0
31	36.154106961430834	38.0	35.0	40.0	29.0	41.0
32	35.93335030243015	38.0	34.0	40.0	28.0	41.0
33	35.977602950696806	38.0	35.0	40.0	29.0	41.0
34	36.01163744644648	38.0	35.0	40.0	29.0	41.0
35	35.80852997644085	38.0	34.0	40.0	27.0	41.0
36	35.750897145522295	38.0	34.0	40.0	27.0	41.0
37	35.5594591673221	38.0	34.0	40.0	27.0	41.0
38	35.48723822636078	38.0	34.0	40.0	26.0	41.0
39	35.461873245495845	38.0	34.0	40.0	26.0	41.0
40	35.39921028944929	38.0	34.0	40.0	26.0	41.0
41	35.281030492404334	38.0	33.0	40.0	26.0	41.0
42	35.17947708947778	38.0	33.0	40.0	25.0	41.0
43	34.71165137704553	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	21.0
15	43.0
16	125.0
17	360.0
18	720.0
19	1470.0
20	2956.0
21	5847.0
22	10200.0
23	17321.0
24	28074.0
25	42187.0
26	61679.0
27	86039.0
28	117764.0
29	154388.0
30	197260.0
31	243574.0
32	295215.0
33	351603.0
34	423301.0
35	507159.0
36	614622.0
37	760234.0
38	1064769.0
39	1035771.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.04820983500293	18.06953112326621	11.867043971711752	28.01521507001911
2	16.599545752885785	23.769503876409185	31.52951170012584	28.10143867057919
3	21.563919781666502	24.357328665669442	28.629423318029602	25.44932823463446
4	16.127001236818025	17.429371115641736	30.836302510181042	35.60732513735919
5	14.158054683993743	36.53290468929866	34.0866981375337	15.222342489173899
6	35.95505879161102	31.02138521294307	15.985769204011548	17.03778679143436
7	26.81547335893309	29.782524918587356	22.195412598940045	21.20658912353951
8	28.356440029601277	31.71756648540284	21.95899090658977	17.96700257840611
9	28.386725384390722	13.170891589886486	17.775610426729614	40.66677259899318
10	18.099766666616855	26.223945919388818	32.71223450295573	22.964052911038596
11	35.977457335257384	21.275245890016674	18.544831872280813	24.20246490244513
12	22.422652382738477	26.040822466552864	28.086661277068988	23.44986387363967
13	33.287523413451716	17.741223930145765	22.51108429041862	26.4601683659839
14	23.77518238043221	20.813278002787115	23.507794576962244	31.903745039818432
15	30.34293681269554	23.023544372776854	21.340100383332228	25.293418431195374
16	24.905902041156537	23.870206001846675	27.25137325985814	23.972518697138646
17	27.07680077068258	25.611414656484545	24.71751517962088	22.594269393211995
18	27.92638467082858	20.470525492415128	27.074791709317715	24.528298127438582
19	26.2235806355043	28.05889970184533	24.256344275412093	21.461175387238278
20	25.553965463737022	22.318994778598945	26.229225931901457	25.89781382576258
21	24.16658406269733	26.15419330125809	24.887820488872705	24.79140214717188
22	25.547191108060435	25.529441632035265	21.02572378926241	27.897643470641885
23	22.451443394363967	23.90100607483704	24.227453640909005	29.42009688988999
24	23.96728849606481	28.71918353074437	23.02269757831728	24.290830394873538
25	26.415537316820508	23.024905885437345	20.534898475155632	30.024658322586518
26	25.271617624814596	23.867449768899828	24.54365665440143	26.317275951884145
27	27.925006554355154	23.00235790747459	21.851215556842902	27.22141998132735
28	24.696860036332463	24.111974121961318	24.906732231803176	26.284433609903047
29	25.831381970218402	22.281138085112143	25.563479448547522	26.32400049612193
30	24.457997583481067	25.78543921983332	26.253401083531624	23.503162113153987
31	28.424598681690462	22.795258548763655	20.825697654860857	27.95444511468503
32	21.94084293905421	25.01809400514353	24.56505896927182	28.476004086530438
33	24.735513712840042	24.067359676610856	25.060433728122195	26.136692882426903
34	23.97258511239038	24.04692038289057	24.99107960150185	26.989414903217206
35	23.85084595596702	26.056778730781293	26.860768560613796	23.23160675263789
36	26.80644088469764	23.95473601348761	23.597222713418358	25.64160038839639
37	24.318990461607584	24.481807451226715	25.586376106581866	25.612825980583832
38	25.821403078645787	23.756254033688805	25.363303879829573	25.059039007835835
39	25.795152450399016	20.38850265652705	24.984670529709785	28.83167436336415
40	25.138372026028797	22.978896719800527	26.208836449619966	25.673894804550706
41	23.818950031323094	22.300132847107275	26.406089747261742	27.474827374307893
42	26.088624843986423	23.369966325806992	26.297799679313954	24.243609150892627
43	25.127114640860356	19.95127445056738	27.410919298329507	27.51069161024276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	109.5
2	165.0
3	857.5
4	1550.0
5	1550.0
6	2278.5
7	3007.0
8	3109.0
9	3211.0
10	3830.5
11	4450.0
12	4450.0
13	6406.0
14	8362.0
15	11622.5
16	14883.0
17	13511.0
18	12139.0
19	12139.0
20	12680.5
21	13222.0
22	12470.0
23	11718.0
24	14268.5
25	16819.0
26	16819.0
27	20524.5
28	24230.0
29	28795.0
30	33360.0
31	41158.0
32	48956.0
33	48956.0
34	62682.5
35	76409.0
36	99730.0
37	123051.0
38	149714.0
39	176377.0
40	176377.0
41	199674.0
42	222971.0
43	259289.5
44	295608.0
45	369395.5
46	443183.0
47	443183.0
48	754801.5
49	1066420.0
50	1076397.5
51	1086375.0
52	787054.5
53	487734.0
54	487734.0
55	443292.5
56	398851.0
57	388052.5
58	377254.0
59	355228.0
60	333202.0
61	333202.0
62	281606.0
63	230010.0
64	200181.0
65	170352.0
66	144770.5
67	119189.0
68	119189.0
69	99998.0
70	80807.0
71	68770.0
72	56733.0
73	43785.0
74	30837.0
75	30837.0
76	24519.0
77	18201.0
78	15745.0
79	13289.0
80	10738.5
81	8188.0
82	8188.0
83	6776.0
84	5364.0
85	4650.5
86	3937.0
87	2652.5
88	1368.0
89	1368.0
90	932.0
91	496.0
92	343.0
93	190.0
94	182.0
95	174.0
96	174.0
97	91.5
98	9.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6022713.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.243064147760116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25905579300003	30.263362274851186
2	9.487417320149852	7.066809837018227
3	3.2391809658037762	3.6191107348690092
4	1.5557554189742047	2.3176439546832692
5	0.947939862005416	1.765209254444329
6	0.5871878345469029	1.3121204513288796
7	0.4594036753268616	1.197672038494055
8	0.32612551470524653	0.9716730771511026
9	0.24897988670227253	0.8345496502759304
>10	1.648961815866613	11.425189333698308
>50	0.11838202750685677	3.0456040858635833
>100	0.09160577173573044	7.187023611517201
>500	0.015569457476440077	4.08175157988368
>1k	0.011801920163331309	8.291560856263112
>5k	0.001225584016961306	3.28887248397462
>10k+	0.0014071520194740923	13.331846775683534
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	87293	1.449396642343741	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	79134	1.3139261326249483	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	49606	0.8236487443449488	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	49135	0.8158283484535955	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	36782	0.6107214472945997	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	32071	0.532500884568134	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	30527	0.5068645973998761	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	28604	0.47493546513008345	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	28030	0.46540487650665074	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	25232	0.41894740792064966	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	23716	0.39377602751451046	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	22926	0.38065901529759094	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	22629	0.3757276828565465	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	22160	0.3679404945910589	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	21718	0.36060160927475704	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	18441	0.3061909142939403	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	17314	0.28747841711866395	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	17189	0.2854029405020628	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	16963	0.28165047877924787	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	15878	0.2636353417471495	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	15310	0.2542043760013137	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	15294	0.25393871499438875	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	14963	0.2484428529136288	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	14163	0.2351598025673812	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	13247	0.21995070992092766	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	12687	0.21065257467855433	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	12597	0.20915823151460144	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	12186	0.20233406439921675	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	11570	0.1921061156326061	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	10891	0.18083212665122844	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	10360	0.17201550198390658	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	9577	0.15901471645751675	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	9268	0.1538841382612786	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	9214	0.15298753236290688	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	8616	0.14305845222908678	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	8614	0.14302524460322116	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	8505	0.14121542899354494	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	8479	0.1407837298572919	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	8470	0.1406342955408966	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	8395	0.1393890095709359	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	8306	0.13791127021991584	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	8255	0.13706447576034256	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	7849	0.1303233277096219	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	7832	0.13004106288976414	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	7699	0.12783275576970046	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	6742	0.11194290679300177	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	6568	0.1090538433426929	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	6403	0.10631421420877933	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	6276	0.10420552996631254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.320762586561903E-5
2	0.0	3.320762586561903E-5	0.0	0.0	3.320762586561903E-5
3	0.0	3.320762586561903E-5	0.0	0.0	3.320762586561903E-5
4	0.0	3.320762586561903E-5	0.0	0.0	3.320762586561903E-5
5	0.0	3.320762586561903E-5	0.0	0.0	3.320762586561903E-5
6	0.0	3.320762586561903E-5	0.0	0.0	4.981143879842855E-5
7	0.0	3.320762586561903E-5	0.0	0.0	4.981143879842855E-5
8	0.0	3.320762586561903E-5	0.0	0.0	4.981143879842855E-5
9	0.0	3.320762586561903E-5	0.0	0.0	4.981143879842855E-5
10	0.0	3.320762586561903E-5	0.0	3.320762586561903E-5	4.981143879842855E-5
11	0.0	3.320762586561903E-5	0.0	3.320762586561903E-5	4.981143879842855E-5
12	0.0	3.320762586561903E-5	0.0	6.641525173123806E-5	6.641525173123806E-5
13	0.0	3.320762586561903E-5	0.0	1.3283050346247612E-4	8.301906466404758E-5
14	0.0	3.320762586561903E-5	0.0	1.4943431639528564E-4	9.96228775968571E-5
15	0.0	3.320762586561903E-5	0.0	2.6566100692495225E-4	1.3283050346247612E-4
16	0.0	3.320762586561903E-5	0.0	7.305677690436187E-4	2.1584956812652372E-4
17	0.0	3.320762586561903E-5	0.0	0.0012120783440950948	2.1584956812652372E-4
18	0.0	3.320762586561903E-5	0.0	0.0018430232355418563	2.3245338105933324E-4
19	0.0	3.320762586561903E-5	0.0	0.003353970212427522	2.3245338105933324E-4
20	0.0	3.320762586561903E-5	0.0	0.004732086685850712	2.4905719399214273E-4
21	0.0	3.320762586561903E-5	0.0	0.008119264524143853	2.6566100692495225E-4
22	0.0	3.320762586561903E-5	0.0	0.012967577900524233	2.6566100692495225E-4
23	0.0	3.320762586561903E-5	0.0	0.019343442066723088	3.154724457233808E-4
24	0.0	3.320762586561903E-5	0.0	0.029704221336796226	3.154724457233808E-4
25	0.0	3.320762586561903E-5	0.0	0.035897443560734175	3.154724457233808E-4
26	0.0	4.981143879842855E-5	0.0	0.050575214193337784	3.320762586561903E-4
27	0.0	4.981143879842855E-5	0.0	0.07609527467106601	3.320762586561903E-4
28	0.0	4.981143879842855E-5	0.0	0.1261225630376211	3.320762586561903E-4
29	0.0	4.981143879842855E-5	0.0	0.17321097651506887	3.320762586561903E-4
30	0.0	4.981143879842855E-5	0.0	0.24887455204988185	3.320762586561903E-4
31	1.6603812932809515E-5	4.981143879842855E-5	0.0	0.4454803009872793	3.320762586561903E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	2280	0.0	18.743422	2
AAAAAGT	2255	0.0	18.62306	1
AAGACGG	1355	0.0	16.520296	5
TGGGCTG	5625	0.0	16.510221	36
GGGCTGT	5995	0.0	16.386154	37
TCGTTAC	590	0.0	16.305084	23
CAAGACG	1410	0.0	16.138296	4
TAAACGG	115	2.2128115E-5	14.478261	5
TACTCCT	5730	0.0	14.431937	37
AAAGTAC	3030	0.0	14.409242	3
TCTTATA	5000	0.0	14.393	37
GACGGAC	1550	0.0	14.083872	7
AAGTACT	3175	0.0	14.0425205	4
AGTACTC	3175	0.0	13.984253	5
ACGGACC	1555	0.0	13.919615	8
TACCTGT	8495	0.0	13.8505	37
GTACTCC	6035	0.0	13.763878	36
CTTATAC	3065	0.0	13.7014675	37
TAACACG	95	0.001246147	13.631579	4
TTAGAGT	775	0.0	13.606452	4
>>END_MODULE