FastQCFastQC Report
Thu 9 Feb 2017
SRR2727193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423772
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC400541.1698793027105776No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG366871.0715374738738443No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA259140.7568845121696188No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG201750.589262369106354No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC173080.5055243164556519No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG172090.5026327687708176No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT151840.4434874752173918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143370.4187486783582552No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG139230.40665675167622145No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT120450.3518049683214887No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA112440.3284097188714669No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT110090.3215459440640323No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC105340.30767235668730275No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC103970.30367092201233026No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG102050.2980630719568943No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT96520.28191129549514393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93380.27274012405031645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78000.22781890850208483No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT76720.22408034179846087No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT75810.2214224545326032No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC74150.2165740008388409No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC74030.21622351021037614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73010.21324433986842584No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT71520.20889241456498855No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC70880.2070231312131766No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC69920.2042192061854586No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT69660.203459809823785No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA68000.19861135613002268No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG59160.17279187983311972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC57690.16849836963442658No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT57440.16776818082512504No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT52580.15357331037230282No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT52200.15246342338216448No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT51400.1501268191923995No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG51240.1496594983544465No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46300.1352309674826478No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG45950.13420870314962563No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG44440.12979836274144424No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG41770.12199994625810365No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT40560.11846583242108412No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39880.11647971885978389No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT39030.1139970769081586No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA37520.10958673649997722No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG36470.10651994350091068No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT36440.10643232084379452No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT35770.10447541483486633No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT35720.10432937707300603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35680.10421254686351779No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC35160.10269375414017055No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG35130.10260613148305436No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG34840.10175911246426456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA20900.017.9688992
AAAAAGT20950.017.8377081
TGGGCTG29500.015.67796636
CAAGACG8500.015.4529424
GGGCTGT31500.014.97619137
AGTACTC26450.014.9678645
GGGTATA1001.0943679E-414.81
AAGACGG8750.014.7999985
TACTCCT27200.014.62316237
AAGTACT26950.014.3469394
AAAGTAC26450.014.3383743
TATAACG1551.2122837E-714.322582
GTACTCC28450.013.98066736
TATCGTC800.006301978613.8750015
TACACCG1601.786484E-713.8750015
TGGAGTG9000.013.77222255
TACACTA1753.572677E-813.7428585
TAGTACT1501.3075442E-613.5666674
ATAACGC6450.013.480623
TTGCGCG4850.013.35051518
Produced by FastQC (version 0.11.5)