##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3423772
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172483156004546	31.0	31.0	33.0	30.0	34.0
2	31.2763685782815	31.0	31.0	34.0	30.0	34.0
3	31.350729546243148	31.0	31.0	34.0	28.0	34.0
4	35.16577943858411	35.0	35.0	37.0	33.0	37.0
5	35.00905609368848	35.0	35.0	37.0	32.0	37.0
6	35.07505552355706	36.0	35.0	37.0	32.0	37.0
7	34.87732944833943	36.0	35.0	37.0	32.0	37.0
8	34.82804637691996	36.0	35.0	37.0	32.0	37.0
9	36.46674603332231	38.0	35.0	39.0	32.0	39.0
10	36.24048914472109	38.0	35.0	39.0	32.0	39.0
11	36.36561342285643	38.0	35.0	39.0	32.0	39.0
12	36.23803804692602	38.0	35.0	39.0	32.0	39.0
13	36.33431986709395	38.0	35.0	39.0	32.0	39.0
14	37.3034206132885	39.0	36.0	41.0	32.0	41.0
15	37.17867836993818	39.0	36.0	41.0	31.0	41.0
16	37.09357778496933	39.0	36.0	40.0	31.0	41.0
17	37.049885039073864	39.0	36.0	40.0	31.0	41.0
18	37.03438868008734	39.0	36.0	40.0	31.0	41.0
19	37.026039409166266	39.0	36.0	40.0	31.0	41.0
20	36.95973884943273	39.0	36.0	40.0	31.0	41.0
21	36.82020853024092	39.0	36.0	40.0	30.0	41.0
22	36.817935890590846	39.0	35.0	40.0	31.0	41.0
23	36.59971779662898	38.0	35.0	40.0	30.0	41.0
24	36.50261670461701	38.0	35.0	40.0	30.0	41.0
25	36.40783293981024	38.0	35.0	40.0	30.0	41.0
26	36.06372591399194	38.0	34.0	40.0	29.0	41.0
27	36.219612170436584	38.0	35.0	40.0	30.0	41.0
28	36.07907594314107	38.0	35.0	40.0	29.0	41.0
29	35.99311782443457	38.0	35.0	40.0	29.0	41.0
30	36.110141679995046	38.0	35.0	40.0	29.0	41.0
31	36.051199378930605	38.0	35.0	40.0	29.0	41.0
32	35.796153774258336	38.0	34.0	40.0	28.0	41.0
33	35.77027705115878	38.0	34.0	40.0	28.0	41.0
34	35.768783669006	38.0	34.0	40.0	28.0	41.0
35	35.50197530676692	38.0	34.0	40.0	27.0	41.0
36	35.38111503920238	38.0	34.0	40.0	26.0	41.0
37	35.15530648653006	38.0	34.0	40.0	25.0	41.0
38	35.02972832303086	38.0	33.0	40.0	25.0	41.0
39	34.96977602480539	38.0	33.0	40.0	25.0	41.0
40	34.84156012725146	38.0	33.0	40.0	24.0	41.0
41	34.69694535734272	38.0	33.0	40.0	24.0	41.0
42	34.54438087582935	38.0	33.0	40.0	23.0	41.0
43	34.08657907126993	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	12.0
14	7.0
15	37.0
16	129.0
17	253.0
18	511.0
19	1000.0
20	2001.0
21	3763.0
22	6471.0
23	10484.0
24	16748.0
25	25425.0
26	37205.0
27	52153.0
28	70665.0
29	93132.0
30	116292.0
31	141521.0
32	168847.0
33	200880.0
34	239709.0
35	285205.0
36	347133.0
37	438056.0
38	588046.0
39	578083.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.328761377802024	18.70939420031474	11.807211461510873	26.15463296037236
2	16.055742029551034	24.971727089303844	32.766025307759975	26.20650557338514
3	21.4194461547089	26.595462548323894	28.92745194481408	23.05763935215312
4	15.471152868824209	17.51252711921238	32.052543218415245	34.96377679354817
5	13.623220237796207	37.93795264404289	33.675723733940224	14.763103384220678
6	33.74588027473792	31.88357752794286	17.35530286479357	17.01523933252565
7	25.88934076217692	31.054988474699837	23.239806856297673	19.81586390682557
8	27.63387865780782	31.945555954076383	23.198624207453065	17.221941180662732
9	28.391259698367765	13.81657423449926	19.83452753279132	37.957638534341655
10	17.96159323693283	26.830670967576115	33.141429978398094	22.06630581709296
11	33.77771650682347	22.534707334483723	20.593923894464936	23.093652264227877
12	21.65468378151349	27.226579339979413	29.303995709994705	21.814741168512388
13	30.961319854242632	18.915803972928106	24.79277241592022	25.330103756909043
14	22.968059789028008	22.03925962359643	25.73226254551997	29.26041804185559
15	28.580057316900774	24.378434077970145	23.340952610162123	23.700555994966955
16	23.06424025898921	25.22010811467586	28.99071550325197	22.724936123082962
17	25.553015796612627	26.572622242368944	26.47162252626635	21.40273943475208
18	25.673234082176034	21.967350629656416	29.320906882818132	23.038508405349422
19	24.581251321641744	28.27402058314631	27.08553606957473	20.05919202563722
20	23.61789862175402	24.048972887213285	28.490682206642266	23.842446284390434
21	22.580796852126834	27.055393875526757	27.93813373086759	22.425675541478814
22	23.855326814986512	26.57639001662494	23.769398195907904	25.798884972480646
23	20.781611626007805	25.845208150542735	26.773482580031615	26.599697643417848
24	21.92158239508939	29.716114273964507	25.704194087690418	22.658109243255684
25	24.24130461958331	25.086600392783165	23.4182357937386	27.253859193894918
26	23.44314983591197	25.615870449317303	26.74082853647965	24.200151178291076
27	25.369271084639983	24.858109710576525	24.353227960273056	25.419391244510443
28	22.784870020550436	26.37888854748505	27.11775199984111	23.718489432123402
29	23.866542515097382	24.32013580343551	27.671030664425082	24.142291017042023
30	22.735334011727417	27.561268682610873	28.298788587557816	21.404608718103894
31	26.34763646644695	24.690049454227676	23.833976094202537	25.12833798512284
32	20.19056759620676	26.755578350427538	27.03150209768641	26.02235195567929
33	22.632465012273016	25.821725278435597	27.50989259798842	24.03591711130297
34	21.965510553856973	25.606699277872476	27.53147697919137	24.89631318907918
35	21.939311379379234	27.535040300580764	28.728636135817453	21.79701218422255
36	24.254214357731765	25.613679882889397	26.234398785900463	23.897706973478375
37	22.600365912216116	26.238779918756272	27.58992129148787	23.570932877539743
38	23.74670392771481	25.396375693241257	27.7113662942509	23.14555408479303
39	23.839846812229318	22.70364381740373	27.230055038711694	26.226454331655262
40	23.287093883588042	24.93147908213514	28.018045594157552	23.76338144011926
41	21.63149298493007	24.726967800425964	28.36269471214789	25.278844502496078
42	23.71296920472508	25.766026476062077	27.879105267523656	22.64189905168919
43	22.56230847147532	22.79944458918409	28.801304526119146	25.836942413221443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	618.0
1	864.5
2	1111.0
3	4219.5
4	7328.0
5	7328.0
6	9795.0
7	12262.0
8	12213.5
9	12165.0
10	13821.0
11	15477.0
12	15477.0
13	21526.5
14	27576.0
15	34929.5
16	42283.0
17	38278.5
18	34274.0
19	34274.0
20	34254.5
21	34235.0
22	29493.5
23	24752.0
24	26596.5
25	28441.0
26	28441.0
27	31286.0
28	34131.0
29	36729.0
30	39327.0
31	44054.5
32	48782.0
33	48782.0
34	58156.5
35	67531.0
36	82225.5
37	96920.0
38	112024.5
39	127129.0
40	127129.0
41	137941.0
42	148753.0
43	167239.5
44	185726.0
45	221491.5
46	257257.0
47	257257.0
48	402215.5
49	547174.0
50	546928.0
51	546682.0
52	397529.5
53	248377.0
54	248377.0
55	220599.5
56	192822.0
57	184196.5
58	175571.0
59	162429.5
60	149288.0
61	149288.0
62	124006.5
63	98725.0
64	85550.5
65	72376.0
66	61752.5
67	51129.0
68	51129.0
69	42783.0
70	34437.0
71	29877.0
72	25317.0
73	19163.5
74	13010.0
75	13010.0
76	10191.0
77	7372.0
78	6519.5
79	5667.0
80	4678.0
81	3689.0
82	3689.0
83	3187.0
84	2685.0
85	2339.5
86	1994.0
87	1384.5
88	775.0
89	775.0
90	552.0
91	329.0
92	228.5
93	128.0
94	130.0
95	132.0
96	132.0
97	68.5
98	5.0
99	7.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3423772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.087709501767286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.70628779790883	34.39299636511806
2	8.297024463728317	6.81811461789451
3	2.7540018910650295	3.3946688900219306
4	1.4088902508768202	2.3155229339159527
5	0.827277879653813	1.6995476598226935
6	0.5565311728753357	1.3719954695867775
7	0.39950798739041904	1.1490407690673272
8	0.28006676601745656	0.9205841538579747
9	0.22090960525570452	0.8169002718206826
>10	1.299917233261305	9.79899558653721
>50	0.11193708300449964	3.222099395723019
>100	0.10848789290822426	9.619513918765936
>500	0.01671452721725753	4.83667492317181
>1k	0.009912944713260473	7.645648945469835
>5k	0.0014471452136146673	4.082842537350071
>10k+	0.0010853589102110006	7.9148535618762175
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	40054	1.1698793027105776	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	36687	1.0715374738738443	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	25914	0.7568845121696188	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	20175	0.589262369106354	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	17308	0.5055243164556519	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	17209	0.5026327687708176	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	15184	0.4434874752173918	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14337	0.4187486783582552	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	13923	0.40665675167622145	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	12045	0.3518049683214887	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	11244	0.3284097188714669	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	11009	0.3215459440640323	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	10534	0.30767235668730275	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	10397	0.30367092201233026	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	10205	0.2980630719568943	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	9652	0.28191129549514393	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9338	0.27274012405031645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7800	0.22781890850208483	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	7672	0.22408034179846087	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	7581	0.2214224545326032	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	7415	0.2165740008388409	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	7403	0.21622351021037614	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7301	0.21324433986842584	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	7152	0.20889241456498855	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	7088	0.2070231312131766	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	6992	0.2042192061854586	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	6966	0.203459809823785	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	6800	0.19861135613002268	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	5916	0.17279187983311972	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	5769	0.16849836963442658	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	5744	0.16776818082512504	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	5258	0.15357331037230282	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	5220	0.15246342338216448	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	5140	0.1501268191923995	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	5124	0.1496594983544465	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	4630	0.1352309674826478	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4595	0.13420870314962563	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	4444	0.12979836274144424	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4177	0.12199994625810365	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	4056	0.11846583242108412	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3988	0.11647971885978389	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	3903	0.1139970769081586	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	3752	0.10958673649997722	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	3647	0.10651994350091068	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	3644	0.10643232084379452	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	3577	0.10447541483486633	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	3572	0.10432937707300603	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3568	0.10421254686351779	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	3516	0.10269375414017055	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	3513	0.10260613148305436	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	3484	0.10175911246426456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.841510474412432E-5
2	0.0	0.0	0.0	0.0	5.841510474412432E-5
3	0.0	0.0	0.0	0.0	5.841510474412432E-5
4	0.0	0.0	0.0	0.0	5.841510474412432E-5
5	0.0	0.0	0.0	0.0	5.841510474412432E-5
6	0.0	0.0	0.0	0.0	8.762265711618648E-5
7	0.0	0.0	0.0	0.0	8.762265711618648E-5
8	0.0	0.0	0.0	0.0	8.762265711618648E-5
9	0.0	0.0	0.0	0.0	1.1683020948824864E-4
10	0.0	0.0	0.0	1.1683020948824864E-4	1.1683020948824864E-4
11	0.0	0.0	0.0	2.0445286660443512E-4	1.1683020948824864E-4
12	0.0	0.0	0.0	4.6732083795299455E-4	1.1683020948824864E-4
13	0.0	0.0	0.0	5.54943495069181E-4	1.1683020948824864E-4
14	0.0	2.920755237206216E-5	0.0	9.930567806501133E-4	2.0445286660443512E-4
15	0.0	2.920755237206216E-5	0.0	0.0016940380375796052	3.2128307609268376E-4
16	2.920755237206216E-5	2.920755237206216E-5	0.0	0.003913812017856329	3.2128307609268376E-4
17	2.920755237206216E-5	2.920755237206216E-5	0.0	0.006484076626597799	3.2128307609268376E-4
18	2.920755237206216E-5	2.920755237206216E-5	0.0	0.008207322216549467	3.2128307609268376E-4
19	2.920755237206216E-5	2.920755237206216E-5	0.0	0.01299736080556766	3.2128307609268376E-4
20	2.920755237206216E-5	2.920755237206216E-5	0.0	0.016998795480540178	3.796981808368081E-4
21	2.920755237206216E-5	2.920755237206216E-5	0.0	0.027016985944157496	3.796981808368081E-4
22	2.920755237206216E-5	2.920755237206216E-5	0.0	0.04030642227344578	3.796981808368081E-4
23	2.920755237206216E-5	2.920755237206216E-5	0.0	0.05736363285873008	4.6732083795299455E-4
24	2.920755237206216E-5	2.920755237206216E-5	0.0	0.08400092062205076	4.6732083795299455E-4
25	2.920755237206216E-5	2.920755237206216E-5	0.0	0.10085367834073063	4.6732083795299455E-4
26	2.920755237206216E-5	2.920755237206216E-5	0.0	0.13333247657846375	4.6732083795299455E-4
27	2.920755237206216E-5	2.920755237206216E-5	0.0	0.19691731809244306	4.6732083795299455E-4
28	2.920755237206216E-5	2.920755237206216E-5	0.0	0.30168480845103	4.6732083795299455E-4
29	2.920755237206216E-5	2.920755237206216E-5	0.0	0.39143961689037704	4.6732083795299455E-4
30	2.920755237206216E-5	2.920755237206216E-5	0.0	0.5315190380667871	4.6732083795299455E-4
31	2.920755237206216E-5	2.920755237206216E-5	0.0	0.8473695094182674	4.6732083795299455E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	2090	0.0	17.968899	2
AAAAAGT	2095	0.0	17.837708	1
TGGGCTG	2950	0.0	15.677966	36
CAAGACG	850	0.0	15.452942	4
GGGCTGT	3150	0.0	14.976191	37
AGTACTC	2645	0.0	14.967864	5
GGGTATA	100	1.0943679E-4	14.8	1
AAGACGG	875	0.0	14.799998	5
TACTCCT	2720	0.0	14.623162	37
AAGTACT	2695	0.0	14.346939	4
AAAGTAC	2645	0.0	14.338374	3
TATAACG	155	1.2122837E-7	14.32258	2
GTACTCC	2845	0.0	13.980667	36
TATCGTC	80	0.0063019786	13.875001	5
TACACCG	160	1.786484E-7	13.875001	5
TGGAGTG	900	0.0	13.7722225	5
TACACTA	175	3.572677E-8	13.742858	5
TAGTACT	150	1.3075442E-6	13.566667	4
ATAACGC	645	0.0	13.48062	3
TTGCGCG	485	0.0	13.350515	18
>>END_MODULE