FastQCFastQC Report
Thu 9 Feb 2017
SRR2727192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5552540
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC599471.07963202426277No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG562661.0133380398880514No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA390380.7030656240207185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340490.6132148530222205No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG311550.5610945621283232No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG275440.49606126205304235No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC268850.4841928198626214No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT241340.43464792689471843No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG218970.39436005863982976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT217230.39122635766694164No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT202190.36413965500473655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT184860.3329287137057995No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT180350.32480630486227924No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA175910.3168099644487028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170690.3074088615300385No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC158930.28622936529948456No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC152900.27536947054861377No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT152330.2743429133333573No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG150530.2711011537062318No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT124130.2235553458417229No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT122690.22096193814002243No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC117210.21109258105299558No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT116430.20968781854790783No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC113280.204014739200438No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT111570.20093506755466867No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC107430.1934790204122798No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA106620.19202022858007325No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC100820.1815745586704463No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91370.1645553206280369No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG91370.1645553206280369No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC88290.15900830971051086No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT87210.1570632539342355No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT84660.1524707611291409No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG81080.1460232614263022No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT80840.14559102680935213No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT79090.14243931606075777No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC74190.13361452596469364No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG72330.1302647076833305No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70650.12723906536467994No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG70110.12626653747654226No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT69310.124825755420042No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG64290.11578484801550282No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT61180.11018380777085802No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG58910.1060955886855385No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT57770.10404247425502562No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT57080.10279979973129416No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT56940.1025476628714066No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA56900.10247562376858159No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT56120.10107086126349381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA34600.017.9118482
AAAAAGT35950.017.1877611
TATCACG2500.017.022
TATAACG1901.8189894E-1216.5526312
TGGGCTG51250.015.59414636
AGTACTC41700.015.4832135
CAAGACG13500.015.3481484
AAGACGG13550.015.018455
ACCGTCG12300.014.8902448
AAAGTAC41750.014.8443113
GGGCTGT55250.014.83348437
TGCGTAA1001.0945625E-414.810
AAGTACT43400.014.7914744
ATACCGT13400.014.7723896
TCTAGCG10700.014.52336528
TACCGTC13050.014.1762457
CGTCGTA13300.013.63157910
TAGAGTG10100.013.5544555
CTAGCGG11250.013.48444529
CGTTATA1102.4590184E-413.4545452
Produced by FastQC (version 0.11.5)