##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727192.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5552540
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14588728761972	31.0	31.0	33.0	30.0	34.0
2	31.239871662338317	31.0	31.0	34.0	28.0	34.0
3	31.31036768758082	31.0	31.0	34.0	28.0	34.0
4	35.129228785384704	35.0	35.0	37.0	32.0	37.0
5	34.97269357807418	35.0	35.0	37.0	32.0	37.0
6	35.04204021943111	36.0	35.0	37.0	32.0	37.0
7	34.84791230680013	35.0	35.0	37.0	32.0	37.0
8	34.80541517935936	35.0	35.0	37.0	32.0	37.0
9	36.43275906161865	38.0	35.0	39.0	32.0	39.0
10	36.213317148548235	38.0	35.0	39.0	32.0	39.0
11	36.33240625011256	38.0	35.0	39.0	32.0	39.0
12	36.21594891707219	38.0	35.0	39.0	32.0	39.0
13	36.30908070900885	38.0	35.0	39.0	32.0	39.0
14	37.26381385816221	39.0	36.0	41.0	32.0	41.0
15	37.152542079840934	39.0	36.0	41.0	31.0	41.0
16	37.07343774200636	39.0	36.0	40.0	31.0	41.0
17	37.04583271799933	39.0	36.0	40.0	31.0	41.0
18	37.03683251268789	39.0	36.0	40.0	31.0	41.0
19	37.02699071055769	39.0	36.0	40.0	31.0	41.0
20	36.96194876579007	39.0	36.0	40.0	31.0	41.0
21	36.818952407366716	39.0	36.0	40.0	30.0	41.0
22	36.80097865121188	39.0	35.0	40.0	30.0	41.0
23	36.578077240326046	38.0	35.0	40.0	30.0	41.0
24	36.465829512259255	38.0	35.0	40.0	30.0	41.0
25	36.37039823216042	38.0	35.0	40.0	30.0	41.0
26	36.01774269073253	38.0	34.0	40.0	29.0	41.0
27	36.1530522967867	38.0	35.0	40.0	29.0	41.0
28	36.00635546974898	38.0	34.0	40.0	29.0	41.0
29	35.904344498193616	38.0	34.0	40.0	28.0	41.0
30	35.984932481350874	38.0	35.0	40.0	29.0	41.0
31	35.899758128712264	38.0	34.0	40.0	29.0	41.0
32	35.626530020495125	38.0	34.0	40.0	27.0	41.0
33	35.569814535329776	38.0	34.0	40.0	27.0	41.0
34	35.53962276723806	38.0	34.0	40.0	27.0	41.0
35	35.24873733462523	38.0	34.0	40.0	26.0	41.0
36	35.10662417560252	38.0	34.0	40.0	25.0	41.0
37	34.860220727811054	38.0	33.0	40.0	24.0	41.0
38	34.703587007027416	38.0	33.0	40.0	24.0	41.0
39	34.6294351774143	38.0	33.0	40.0	24.0	41.0
40	34.45987530031301	38.0	33.0	40.0	23.0	41.0
41	34.28684818119275	38.0	33.0	40.0	22.0	41.0
42	34.10566263367756	38.0	33.0	40.0	21.0	41.0
43	33.63667546744373	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	4.0
13	13.0
14	26.0
15	76.0
16	228.0
17	501.0
18	998.0
19	1925.0
20	3944.0
21	6974.0
22	11911.0
23	19181.0
24	30055.0
25	44731.0
26	64701.0
27	89941.0
28	121906.0
29	157565.0
30	195641.0
31	235122.0
32	278449.0
33	328572.0
34	391363.0
35	464009.0
36	566936.0
37	717011.0
38	929880.0
39	890873.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.608708807140516	19.017350617915405	11.947090880930169	25.42684969401391
2	16.17018517651381	25.489739830780145	32.324305633097644	26.01576935960839
3	21.629416447247564	27.045028041220775	28.825132281802563	22.500423229729098
4	15.566767641475792	17.623484027129926	31.817510544723675	34.99223778667061
5	13.69214809798759	37.89879946835142	33.48163182975719	14.9274206039038
6	33.35075478969985	31.900643669383744	17.436488525971896	17.31211301494451
7	25.551315253919824	30.99775958390214	23.639235376962613	19.811689785215414
8	27.76041235182457	31.33054421940229	23.46106466590065	17.447978762872488
9	27.82928173412528	13.812129223742648	20.65670846135282	37.701880580779246
10	18.19059385434414	26.88099500408822	32.93067677135149	21.997734370216154
11	33.05514233125741	22.713226739474187	21.06327914792149	23.168351781346917
12	21.547471967784112	27.843149261419097	29.44737003245362	21.16200873834317
13	30.673331484329697	18.82529436978392	25.562265197549227	24.939108948337157
14	22.759511863039258	22.211024143905313	26.388967931793378	28.640496061262056
15	27.98450799093748	24.531205538366223	23.987004145850367	23.497282324845926
16	22.55852276615747	25.178530906576093	29.477356309004527	22.78559001826191
17	24.76965496871702	27.090034470710698	27.11643680189607	21.02387375867621
18	25.085366336847642	21.863435472774622	30.15540635456926	22.895791835808478
19	23.948841431128816	28.33411375694727	27.986687173797936	19.730357638125977
20	23.28278589618445	24.276961534721046	29.35173812345341	23.088514445641096
21	22.44234170307643	27.136877897322663	28.383982825877887	22.036797573723018
22	23.433275581985903	26.96382196256128	24.676346320782923	24.9265561346699
23	20.416764219618408	26.067727562520936	27.53492995998228	25.980578257878378
24	21.576989990166663	30.203258328620773	26.387437100858346	21.83231458035422
25	23.550195045870897	25.73305190057163	24.3597704834184	26.35698257013907
26	22.932657846679177	26.183584449639262	27.47837926426464	23.405378439416914
27	24.58103858774543	25.33141589254647	25.291452200254298	24.7960933194538
28	22.206359611997392	26.62417560251705	28.34906187078346	22.8204029147021
29	23.16568633454239	24.686755971141135	28.514247533561214	23.633310160755258
30	21.694936011266915	28.15399799010903	29.313125164339205	20.837940834284847
31	25.47126180090553	25.3421497188674	24.55092624276457	24.635662237462494
32	19.48805411577404	27.297290969538263	27.791010960749496	25.423643953938196
33	21.92083262795045	26.469399590097503	28.363379642469933	23.24638813948211
34	21.301404402309572	26.150068257049924	28.067118832102068	24.481408508538436
35	21.265618977981248	28.060581283520698	29.432962211888615	21.240837526609443
36	23.48341479755211	26.269707197066566	26.835808476841226	23.41106952854009
37	21.87989640777014	26.72847021363196	28.123939674455293	23.26769370414261
38	22.729687674469705	26.026449156602204	28.635255216531537	22.60860795239656
39	23.196411011897258	23.194934210289343	27.77507230924946	25.833582468563936
40	22.297669174828098	25.610063142273624	28.705817517748635	23.386450165149643
41	20.81092617072547	25.238053215285255	29.169569242184657	24.781451371804618
42	22.888497876647445	26.30334945808585	28.420020387066096	22.388132278200608
43	21.78282371671343	23.48318067046793	29.599102392778796	25.13489322003984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1143.0
1	1445.5
2	1748.0
3	8034.5
4	14321.0
5	14321.0
6	20165.0
7	26009.0
8	25811.0
9	25613.0
10	29638.0
11	33663.0
12	33663.0
13	47670.0
14	61677.0
15	79956.5
16	98236.0
17	88381.0
18	78526.0
19	78526.0
20	77600.5
21	76675.0
22	64272.0
23	51869.0
24	53610.0
25	55351.0
26	55351.0
27	58967.5
28	62584.0
29	65804.5
30	69025.0
31	75867.5
32	82710.0
33	82710.0
34	97251.0
35	111792.0
36	135692.0
37	159592.0
38	183350.0
39	207108.0
40	207108.0
41	224928.0
42	242748.0
43	269484.5
44	296221.0
45	353049.0
46	409877.0
47	409877.0
48	629189.5
49	848502.0
50	845453.0
51	842404.0
52	606379.0
53	370354.0
54	370354.0
55	331017.5
56	291681.0
57	281485.5
58	271290.0
59	254159.0
60	237028.0
61	237028.0
62	197745.0
63	158462.0
64	138874.0
65	119286.0
66	101943.0
67	84600.0
68	84600.0
69	71415.0
70	58230.0
71	50562.0
72	42894.0
73	32519.0
74	22144.0
75	22144.0
76	17319.0
77	12494.0
78	11122.5
79	9751.0
80	8088.5
81	6426.0
82	6426.0
83	5531.0
84	4636.0
85	4052.5
86	3469.0
87	2390.0
88	1311.0
89	1311.0
90	946.5
91	582.0
92	408.0
93	234.0
94	239.5
95	245.0
96	245.0
97	130.5
98	16.0
99	14.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5552540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.01478535264472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.33855788024613	30.477440461836242
2	9.032812809056619	6.68695254515701
3	2.9882337674809785	3.3182649446049983
4	1.4799712404169656	2.191232711684853
5	0.8855834510052065	1.638984067540604
6	0.5601099958482278	1.2439410762115726
7	0.42452714867831376	1.099964689928865
8	0.31572767217168013	0.9349273612259935
9	0.23714070144344782	0.7899940946074345
>10	1.4671881759299463	10.027439113340453
>50	0.12078016775618411	3.099331532171425
>100	0.11163302125460786	8.786492219407698
>500	0.019562083881969724	4.992814185376007
>1k	0.015441137109440997	10.381560161904199
>5k	0.0013405488808839448	3.34945060207162
>10k+	0.001390198839435202	10.98121023293103
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	59947	1.07963202426277	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	56266	1.0133380398880514	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	39038	0.7030656240207185	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34049	0.6132148530222205	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	31155	0.5610945621283232	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	27544	0.49606126205304235	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	26885	0.4841928198626214	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	24134	0.43464792689471843	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	21897	0.39436005863982976	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21723	0.39122635766694164	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	20219	0.36413965500473655	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18486	0.3329287137057995	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	18035	0.32480630486227924	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	17591	0.3168099644487028	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17069	0.3074088615300385	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	15893	0.28622936529948456	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	15290	0.27536947054861377	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	15233	0.2743429133333573	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	15053	0.2711011537062318	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	12413	0.2235553458417229	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	12269	0.22096193814002243	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	11721	0.21109258105299558	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	11643	0.20968781854790783	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	11328	0.204014739200438	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	11157	0.20093506755466867	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	10743	0.1934790204122798	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	10662	0.19202022858007325	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	10082	0.1815745586704463	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9137	0.1645553206280369	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	9137	0.1645553206280369	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	8829	0.15900830971051086	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	8721	0.1570632539342355	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	8466	0.1524707611291409	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	8108	0.1460232614263022	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	8084	0.14559102680935213	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	7909	0.14243931606075777	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	7419	0.13361452596469364	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	7233	0.1302647076833305	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7065	0.12723906536467994	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	7011	0.12626653747654226	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	6931	0.124825755420042	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	6429	0.11578484801550282	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	6118	0.11018380777085802	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	5891	0.1060955886855385	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5777	0.10404247425502562	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	5708	0.10279979973129416	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	5694	0.1025476628714066	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	5690	0.10247562376858159	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	5612	0.10107086126349381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.8009775706253354E-5
7	0.0	0.0	0.0	0.0	1.8009775706253354E-5
8	0.0	0.0	0.0	0.0	1.8009775706253354E-5
9	0.0	0.0	0.0	0.0	3.601955141250671E-5
10	0.0	0.0	0.0	1.2606842994377347E-4	3.601955141250671E-5
11	0.0	0.0	0.0	3.06166187006307E-4	3.601955141250671E-5
12	0.0	0.0	0.0	6.483519254251208E-4	3.601955141250671E-5
13	0.0	0.0	0.0	8.10439906781401E-4	3.601955141250671E-5
14	0.0	1.8009775706253354E-5	0.0	0.0015668504864440418	3.601955141250671E-5
15	0.0	1.8009775706253354E-5	0.0	0.002593407701700483	3.601955141250671E-5
16	0.0	1.8009775706253354E-5	0.0	0.00547497181470102	7.203910282501342E-5
17	0.0	1.8009775706253354E-5	0.0	0.00907692695595169	7.203910282501342E-5
18	0.0	1.8009775706253354E-5	0.0	0.01149023690058964	9.004887853126677E-5
19	0.0	1.8009775706253354E-5	0.0	0.017577541089303275	9.004887853126677E-5
20	0.0	1.8009775706253354E-5	0.0	0.023106542231123052	9.004887853126677E-5
21	0.0	1.8009775706253354E-5	0.0	0.03592950253397544	9.004887853126677E-5
22	0.0	1.8009775706253354E-5	0.0	0.05266058416508481	9.004887853126677E-5
23	0.0	1.8009775706253354E-5	0.0	0.07484862783518895	1.4407820565002683E-4
24	0.0	1.8009775706253354E-5	0.0	0.10993167091097047	1.4407820565002683E-4
25	0.0	1.8009775706253354E-5	0.0	0.13323632067486232	1.620879813562802E-4
26	0.0	1.8009775706253354E-5	0.0	0.17453273636930125	1.620879813562802E-4
27	0.0	1.8009775706253354E-5	0.0	0.2524790456259658	1.620879813562802E-4
28	0.0	1.8009775706253354E-5	0.0	0.3926311201720294	1.620879813562802E-4
29	0.0	1.8009775706253354E-5	0.0	0.5042917295508001	1.620879813562802E-4
30	0.0	1.8009775706253354E-5	0.0	0.6841553595291524	1.620879813562802E-4
31	0.0	1.8009775706253354E-5	0.0	1.0458456850378386	1.620879813562802E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	3460	0.0	17.911848	2
AAAAAGT	3595	0.0	17.187761	1
TATCACG	250	0.0	17.02	2
TATAACG	190	1.8189894E-12	16.552631	2
TGGGCTG	5125	0.0	15.594146	36
AGTACTC	4170	0.0	15.483213	5
CAAGACG	1350	0.0	15.348148	4
AAGACGG	1355	0.0	15.01845	5
ACCGTCG	1230	0.0	14.890244	8
AAAGTAC	4175	0.0	14.844311	3
GGGCTGT	5525	0.0	14.833484	37
TGCGTAA	100	1.0945625E-4	14.8	10
AAGTACT	4340	0.0	14.791474	4
ATACCGT	1340	0.0	14.772389	6
TCTAGCG	1070	0.0	14.523365	28
TACCGTC	1305	0.0	14.176245	7
CGTCGTA	1330	0.0	13.631579	10
TAGAGTG	1010	0.0	13.554455	5
CTAGCGG	1125	0.0	13.484445	29
CGTTATA	110	2.4590184E-4	13.454545	2
>>END_MODULE