FastQCFastQC Report
Thu 9 Feb 2017
SRR2727191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6623534
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC568010.8575633491124224No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG515590.7784213080207636No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA364880.5508841654621234No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG279900.4225840767179575No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC273170.41242333775292767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256640.3874668719146003No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG238370.3598834096722384No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT214390.3236791718741083No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG200470.3026632006418326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168630.25459218598409855No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT164840.24887016508105794No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA160490.2423026740709718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152330.22998296679687913No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC147570.22279647088699175No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT145720.22000339999764476No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG141160.2131188576974165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137760.20798564633321123No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC135380.20439239837826756No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT131030.19782490736818137No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT113590.17149455260590496No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC106570.16089598090686935No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC105890.1598693386340283No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT102210.15431339221630022No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC101020.1525167682388284No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA97650.147428849916072No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT95900.14478675583155456No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC93690.14145016844482114No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT91440.1380531903361559No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG82210.12411803125038688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC82030.12384627300169365No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT76870.11605586987248802No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT75870.11454610182419234No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT74380.1122965474322318No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG72930.11010738376220307No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72620.10963935566723142No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.10951857422336778No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT68580.10353989275211692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT30650.016.1761821
CAAGACG18700.015.9278074
AAGACGG18800.015.8430855
AAAAGTA31600.015.63133052
TATCACG2650.015.3584912
AGTACTC35150.014.8947375
AAGTACT36550.014.4254444
AAAGTAC35000.014.32428553
TATACTG5500.013.790915
TACTCCT41650.013.63625437
GTACTCC42000.013.34642836
ACGGACC21650.013.1593538
GACGGAC21800.013.1536697
CACTCTA20400.013.1495099
GGTTATC14750.013.0440682
AACGCTT19650.012.89821830
TGGGCTG47650.012.88982136
TCTATAC4600.012.8695643
ACCGTCG16200.012.7901238
GGGCTGT51300.012.7300237
Produced by FastQC (version 0.11.5)