FastQCFastQC Report
Thu 9 Feb 2017
SRR2727190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5617632
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC744401.3251134997806904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG699421.2450441751969512No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA488130.8689248423535041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT472050.8403006818531367No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG377830.6725787662844416No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG342840.6102927354444008No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC334550.5955356278232536No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT302110.5377888761670397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT295630.5262537667116678No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG287530.5118348798924529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT257460.45830698771297235No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239850.42695925970230875No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT219840.3913392689303963No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA217440.3870670061691474No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC196550.34988051905144374No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT195200.34747737124824124No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT188150.3349275993870727No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG185890.33090455195356333No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC183680.3269705099942467No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC151970.270523238261246No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT147670.26286876748067517No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC140050.24930433321370998No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC138300.24618914161696603No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT134790.23994095732863951No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT127500.2269639591913461No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA126520.2252194518971695No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC126030.22434719825008115No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT125180.22283410518880556No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121040.21546445192565122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC113860.2026832658315817No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG112490.20024451583870218No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT104900.1867334848562526No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG100670.17920362173955146No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT99090.17639104875506262No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT97510.1735784757705738No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT95960.17081930607060059No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92930.16542557433452387No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG92400.1644821163080814No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG91810.1634318517126077No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC88350.15727267289847396No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG79680.1418391236734624No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT77000.1370684302567345No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT74370.13238674231419928No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT74220.13211972589162124No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA72840.12966317480390316No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG72450.1289689321052002No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT70920.12624536459490404No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.12489248138717524No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT66160.11777204345176046No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG65780.11709560184789605No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA63600.113214963173095No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG62640.11150605806859545No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG61770.10995736281764273No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61370.10924531902410126No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT60590.10785683362669538No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC60540.10776782815250269No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA57760.10281912378738942No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC57020.1015018427693377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA36600.019.1571042
AAAAAGT37750.018.4754961
TGGGCTG53500.016.35607536
GGGCTGT56350.016.349637
ACCGTCG9850.015.5888328
AAGTACT47900.015.410234
AGTACTC46900.015.344355
AAGACGG13400.015.3246275
AAAGTAC47200.015.0508473
ATACCGT10900.014.596336
CAAGACG14000.014.4035724
TACCGTC10650.014.2441317
CCGTCGT10550.014.2037919
TAGACAG4450.014.134835
TATCACG3450.013.9420282
TATACCG1203.3048513E-513.8749995
CGTCGTA10900.013.74770610
TACTCCT48650.013.65159237
GTATAAC5150.013.6504861
CGAACGT5650.013.4247794
Produced by FastQC (version 0.11.5)