##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727190.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5617632
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136237119127774	31.0	31.0	33.0	30.0	34.0
2	31.2120747674465	31.0	31.0	34.0	28.0	34.0
3	31.285263790864192	31.0	31.0	34.0	28.0	34.0
4	35.09450708056348	35.0	35.0	37.0	32.0	37.0
5	34.949332031717276	35.0	35.0	37.0	32.0	37.0
6	35.03791775609367	36.0	35.0	37.0	32.0	37.0
7	34.83912100329819	35.0	35.0	37.0	32.0	37.0
8	34.81641107854698	36.0	35.0	37.0	32.0	37.0
9	36.42644053579871	38.0	35.0	39.0	32.0	39.0
10	36.21499913130657	38.0	35.0	39.0	32.0	39.0
11	36.33539559017038	38.0	35.0	39.0	32.0	39.0
12	36.23976686262112	38.0	35.0	39.0	32.0	39.0
13	36.327681485722096	38.0	35.0	39.0	32.0	39.0
14	37.267918048031625	39.0	36.0	41.0	32.0	41.0
15	37.163156824797355	39.0	36.0	41.0	31.0	41.0
16	37.076328780525316	39.0	36.0	40.0	31.0	41.0
17	37.08405819391516	39.0	36.0	40.0	31.0	41.0
18	37.07907566746985	39.0	36.0	40.0	31.0	41.0
19	37.07097492324168	39.0	36.0	40.0	31.0	41.0
20	37.03721835107746	39.0	36.0	40.0	31.0	41.0
21	36.896702916816196	39.0	36.0	40.0	31.0	41.0
22	36.89625788944523	39.0	35.0	40.0	31.0	41.0
23	36.66490044203679	39.0	35.0	40.0	30.0	41.0
24	36.56101236250434	38.0	35.0	40.0	30.0	41.0
25	36.47995917140888	38.0	35.0	40.0	30.0	41.0
26	36.11513409920764	38.0	35.0	40.0	29.0	41.0
27	36.227450107091386	38.0	35.0	40.0	30.0	41.0
28	36.08066815341411	38.0	35.0	40.0	29.0	41.0
29	35.98024470097009	38.0	35.0	40.0	29.0	41.0
30	36.023173643271754	38.0	35.0	40.0	29.0	41.0
31	35.90272039891541	38.0	35.0	40.0	29.0	41.0
32	35.59324195675331	38.0	34.0	40.0	27.0	41.0
33	35.5281967562133	38.0	34.0	40.0	27.0	41.0
34	35.47858652898588	38.0	34.0	40.0	27.0	41.0
35	35.18234871917562	38.0	34.0	40.0	25.0	41.0
36	35.017477292923424	38.0	34.0	40.0	25.0	41.0
37	34.74221415713952	38.0	33.0	40.0	24.0	41.0
38	34.5736956425768	38.0	33.0	40.0	24.0	41.0
39	34.47007386742314	38.0	33.0	40.0	23.0	41.0
40	34.27560687492524	38.0	33.0	40.0	22.0	41.0
41	34.0801232619011	38.0	33.0	40.0	21.0	41.0
42	33.88129873939767	38.0	33.0	40.0	20.0	41.0
43	33.3957979091546	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	10.0
13	9.0
14	17.0
15	52.0
16	204.0
17	419.0
18	921.0
19	1866.0
20	3705.0
21	6828.0
22	11636.0
23	19063.0
24	30277.0
25	45272.0
26	65087.0
27	91963.0
28	123915.0
29	161097.0
30	199784.0
31	240166.0
32	283299.0
33	332627.0
34	397539.0
35	471977.0
36	574278.0
37	735414.0
38	933093.0
39	887111.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.363051905144374	18.851679853717723	12.0493474830676	24.735920758070304
2	15.373043303655349	26.537266948066375	32.17264854657621	25.91704120170207
3	22.35466830151922	27.943660246879826	28.72594716065417	20.97572429094679
4	15.84997023656943	18.036692328724985	30.93655832208304	35.17677911262254
5	13.434148053841904	38.55261433999237	33.13009823356175	14.883139372603974
6	32.84832470336256	30.912936269232304	17.977735102619754	18.261003924785392
7	24.5697653388474	31.37936055619165	24.032991125086156	20.017882979874795
8	28.016840547761053	31.05532722684576	24.16573388929713	16.762098336096063
9	28.059207153476766	13.884658162015597	21.22020452745926	36.83593015704838
10	18.543418294398776	27.506002529179558	32.841257668711656	21.10932150771001
11	31.67001683271528	23.229485306264277	21.116922575206065	23.98357528581438
12	21.297336671394635	28.812460481569456	29.744899630306865	20.14530321672904
13	30.667690585641775	18.99773783686792	26.05464366480396	24.279927912686343
14	22.559683510774647	22.64176435907514	27.492331288343557	27.306220841806656
15	28.46861453366828	23.856226253339486	24.98310676099823	22.69205245199401
16	21.31296603266287	25.30028666883128	31.26116128646376	22.125586012042085
17	24.53619247398192	27.463173094998034	28.05869092172645	19.941943509293594
18	24.50188976422806	21.606577290929703	31.62989316494922	22.261639779893024
19	23.30563838998354	28.9684692767344	29.343538344982367	18.382353988299695
20	22.565095043605563	24.38470515690597	31.01648523790807	22.033714561580396
21	21.20801077749486	27.654303450279404	30.06923557826501	21.06845019396073
22	22.712132086971877	27.634935859095076	25.362270081059062	24.29066197287398
23	19.378574459843577	26.775374392626645	28.720464423443897	25.12558672408588
24	20.500168042335275	31.3334693336979	27.467516562138638	20.698846061828185
25	22.68518122938633	26.316212952361422	25.027912116706823	25.97069370154542
26	22.07586755415805	26.58232151910271	28.602567772328268	22.739243154410968
27	23.69630121731007	25.891888254695218	26.18396861880593	24.227841909188783
28	21.20824219172776	27.4679615895096	29.486926163906784	21.836870054855854
29	22.465337708130402	24.89472432512489	29.85086954788067	22.789068418864034
30	20.795701818844666	28.979327944585904	30.697258204168588	19.52771203240084
31	25.059936286321356	25.73765956901413	25.398068082779364	23.804336061885152
32	18.021045166361912	28.191042061850975	29.220835398260338	24.56707737352678
33	20.975510677808728	27.311561170258216	29.686601044710653	22.0263271072224
34	19.847152679278388	26.953741362908783	29.27420308058627	23.92490287722656
35	19.69596798081469	29.327944585903808	30.751729554374513	20.224357878906986
36	22.76941601016229	27.035662001355732	27.614215384703023	22.580706603778957
37	20.6259149762747	27.919326150235545	29.101372250798914	22.35338662269084
38	21.985865218654407	27.1995388804393	29.266317195572793	21.548278705333495
39	22.329711166555587	23.963905076017795	28.54099378528177	25.165389972144848
40	21.397770448473665	26.67308930168441	29.374583454380776	22.55455679546115
41	19.87826899305615	26.323903025331667	29.470157532568887	24.3276704490433
42	21.925127883065322	27.632781926619614	28.859312963184486	21.582777227130578
43	21.115605294188015	24.334381461797427	29.9318111261115	24.61820211790306
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1403.0
1	1860.5
2	2318.0
3	10404.0
4	18490.0
5	18490.0
6	26359.0
7	34228.0
8	34570.0
9	34912.0
10	40648.5
11	46385.0
12	46385.0
13	65883.5
14	85382.0
15	110081.0
16	134780.0
17	119731.5
18	104683.0
19	104683.0
20	102180.5
21	99678.0
22	81529.5
23	63381.0
24	63807.5
25	64234.0
26	64234.0
27	66258.5
28	68283.0
29	70111.5
30	71940.0
31	76671.5
32	81403.0
33	81403.0
34	93379.0
35	105355.0
36	125327.5
37	145300.0
38	166613.5
39	187927.0
40	187927.0
41	205753.5
42	223580.0
43	253861.0
44	284142.0
45	348819.0
46	413496.0
47	413496.0
48	674929.0
49	936362.0
50	931200.5
51	926039.0
52	640525.5
53	355012.0
54	355012.0
55	307622.5
56	260233.0
57	248189.5
58	236146.0
59	219234.0
60	202322.0
61	202322.0
62	168944.0
63	135566.0
64	117286.5
65	99007.0
66	83632.0
67	68257.0
68	68257.0
69	57303.5
70	46350.0
71	40105.0
72	33860.0
73	25666.5
74	17473.0
75	17473.0
76	13667.5
77	9862.0
78	8605.5
79	7349.0
80	6122.5
81	4896.0
82	4896.0
83	4137.5
84	3379.0
85	2977.5
86	2576.0
87	1733.0
88	890.0
89	890.0
90	652.5
91	415.0
92	284.5
93	154.0
94	156.0
95	158.0
96	158.0
97	83.0
98	8.0
99	13.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5617632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.371937900001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.69090504874549	28.078747153303585
2	9.184310101337699	6.313650729150624
3	3.1020441543610904	3.198698071102815
4	1.5074130388991196	2.0725082945076934
5	0.94220052703186	1.619262900224365
6	0.6351911295835627	1.3099650036406623
7	0.4474258341395172	1.0765225090129742
8	0.3282791513882082	0.9026872484304415
9	0.2552929536064947	0.7897422192903242
>10	1.6113339367763606	10.292541427452743
>50	0.13554325495805372	3.2473524284348105
>100	0.12100409156853681	8.75523725023796
>500	0.02201574460988152	5.288280638694358
>1k	0.01375984030578935	8.709818203803204
>5k	0.001534751418722658	3.7934782500386945
>10k+	0.0017464412695809557	14.551507672674827
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	74440	1.3251134997806904	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	69942	1.2450441751969512	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	48813	0.8689248423535041	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47205	0.8403006818531367	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	37783	0.6725787662844416	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	34284	0.6102927354444008	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	33455	0.5955356278232536	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	30211	0.5377888761670397	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29563	0.5262537667116678	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	28753	0.5118348798924529	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25746	0.45830698771297235	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23985	0.42695925970230875	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	21984	0.3913392689303963	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	21744	0.3870670061691474	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	19655	0.34988051905144374	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	19520	0.34747737124824124	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	18815	0.3349275993870727	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	18589	0.33090455195356333	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	18368	0.3269705099942467	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	15197	0.270523238261246	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	14767	0.26286876748067517	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	14005	0.24930433321370998	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	13830	0.24618914161696603	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	13479	0.23994095732863951	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	12750	0.2269639591913461	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	12652	0.2252194518971695	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	12603	0.22434719825008115	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	12518	0.22283410518880556	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12104	0.21546445192565122	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	11386	0.2026832658315817	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	11249	0.20024451583870218	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	10490	0.1867334848562526	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	10067	0.17920362173955146	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	9909	0.17639104875506262	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	9751	0.1735784757705738	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	9596	0.17081930607060059	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9293	0.16542557433452387	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	9240	0.1644821163080814	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	9181	0.1634318517126077	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	8835	0.15727267289847396	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	7968	0.1418391236734624	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	7700	0.1370684302567345	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	7437	0.13238674231419928	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	7422	0.13211972589162124	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	7284	0.12966317480390316	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	7245	0.1289689321052002	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	7092	0.12624536459490404	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7016	0.12489248138717524	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	6616	0.11777204345176046	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	6578	0.11709560184789605	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	6360	0.113214963173095	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	6264	0.11150605806859545	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	6177	0.10995736281764273	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6137	0.10924531902410126	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	6059	0.10785683362669538	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	6054	0.10776782815250269	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	5776	0.10281912378738942	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC	5702	0.1015018427693377	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.780109483853695E-5	0.0
5	0.0	0.0	0.0	1.780109483853695E-5	0.0
6	0.0	0.0	0.0	1.780109483853695E-5	0.0
7	0.0	0.0	0.0	1.780109483853695E-5	0.0
8	0.0	0.0	0.0	1.780109483853695E-5	0.0
9	0.0	0.0	0.0	5.340328451561085E-5	3.56021896770739E-5
10	0.0	0.0	0.0	1.780109483853695E-4	3.56021896770739E-5
11	0.0	0.0	0.0	4.4502737096342374E-4	3.56021896770739E-5
12	0.0	0.0	0.0	9.612591212809952E-4	5.340328451561085E-5
13	0.0	0.0	0.0	0.001441888681921493	7.12043793541478E-5
14	0.0	0.0	0.0	0.0025455565619107836	8.900547419268474E-5
15	0.0	0.0	0.0	0.004468074804472775	1.068065690312217E-4
16	0.0	0.0	0.0	0.00922096712636214	1.2460766386975864E-4
17	0.0	0.0	0.0	0.015148731707594943	1.2460766386975864E-4
18	0.0	1.780109483853695E-5	0.0	0.018993768192718925	1.2460766386975864E-4
19	1.780109483853695E-5	1.780109483853695E-5	0.0	0.02778750904295618	1.2460766386975864E-4
20	1.780109483853695E-5	1.780109483853695E-5	0.0	0.035263968875141695	1.2460766386975864E-4
21	1.780109483853695E-5	1.780109483853695E-5	0.0	0.053260875756902554	1.2460766386975864E-4
22	1.780109483853695E-5	1.780109483853695E-5	0.0	0.0769897351766723	1.2460766386975864E-4
23	1.780109483853695E-5	1.780109483853695E-5	0.0	0.11072280989569983	1.6020985354683255E-4
24	1.780109483853695E-5	1.780109483853695E-5	0.0	0.1633250451435765	1.780109483853695E-4
25	1.780109483853695E-5	1.780109483853695E-5	0.0	0.1970047165780884	1.780109483853695E-4
26	1.780109483853695E-5	1.780109483853695E-5	0.0	0.252455127000131	1.780109483853695E-4
27	1.780109483853695E-5	1.780109483853695E-5	0.0	0.3448072070224607	1.9581204322390643E-4
28	1.780109483853695E-5	1.780109483853695E-5	0.0	0.5077762302692664	1.9581204322390643E-4
29	1.780109483853695E-5	1.780109483853695E-5	0.0	0.6317786569145148	1.9581204322390643E-4
30	1.780109483853695E-5	1.780109483853695E-5	0.0	0.8319519683738629	1.9581204322390643E-4
31	3.56021896770739E-5	1.780109483853695E-5	0.0	1.193848226441319	1.9581204322390643E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAAGTA	3660	0.0	19.157104	2
AAAAAGT	3775	0.0	18.475496	1
TGGGCTG	5350	0.0	16.356075	36
GGGCTGT	5635	0.0	16.3496	37
ACCGTCG	985	0.0	15.588832	8
AAGTACT	4790	0.0	15.41023	4
AGTACTC	4690	0.0	15.34435	5
AAGACGG	1340	0.0	15.324627	5
AAAGTAC	4720	0.0	15.050847	3
ATACCGT	1090	0.0	14.59633	6
CAAGACG	1400	0.0	14.403572	4
TACCGTC	1065	0.0	14.244131	7
CCGTCGT	1055	0.0	14.203791	9
TAGACAG	445	0.0	14.13483	5
TATCACG	345	0.0	13.942028	2
TATACCG	120	3.3048513E-5	13.874999	5
CGTCGTA	1090	0.0	13.747706	10
TACTCCT	4865	0.0	13.651592	37
GTATAAC	515	0.0	13.650486	1
CGAACGT	565	0.0	13.424779	4
>>END_MODULE