FastQCFastQC Report
Thu 9 Feb 2017
SRR2727189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4043708
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC429071.0610805725833814No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG395140.9771724367832693No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA309250.7647683759559295No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG230210.5693042128660131No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG229930.5686117790898848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219010.5416068618208831No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC194560.48114255529825595No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT192780.47674065486429784No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG192390.47577619353326206No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA137890.3409989049654426No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC125470.3102845210386111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123800.3061546481595605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121890.30143126061525705No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG118940.29413597618819165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116990.2893136695330128No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT112170.2773939166725194No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC111470.2756628322321988No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT105360.2605529380459717No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC99710.24658061363481237No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT92450.22862679501091573No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC88740.21945204747721644No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC87810.21715217814936194No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC86790.21462974082203762No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT85950.21255243949365285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC76640.18952901643738865No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71820.17760926357689527No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG71630.17713939780023683No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT68730.1699677622617657No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT68320.16895384137529218No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT66740.16504653649571135No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT66350.1640820751646756No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT65910.1629939649450455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61510.15211286274874447No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA61390.1518161054161181No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60430.14944204675510694No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT60280.14907110008932395No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG58790.14538636320921294No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC52670.13025173924526698No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT52560.12997971169035943No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG51320.12691321925322005No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT47820.11825779705161699No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG47590.11768901216408305No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG46940.11608157661235677No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA46710.11551279172482287No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT44810.11081413395823833No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG43900.10856372418582153No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT42490.10507682552746142No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG41770.10329628153170307No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT41430.10245546908926163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGT28650.019.694591
AAAAGTA30600.018.741832
AAGTACT37150.015.8358024
AGTACTC37250.015.7932885
AAAGTAC36650.015.6984993
AAGACGG11350.014.9955955
CAAGACG11750.014.6425534
TGCGATT1051.6572591E-414.09523812
GTACCGT1356.576298E-613.7037036
CGCGGTC5550.013.66666810
GTAATAC1907.1413524E-913.6315793
TCTATAC2457.2759576E-1213.5918363
GGGTATA1102.458759E-413.4545451
TGTGCGA1652.5998452E-713.45454510
TTATCTA8000.013.41254
ACACGCT6850.013.2335779
CGAACGA6050.013.14876116
AGGTTAT8050.013.099381
GCGCAAG13350.012.887641
CGCAAGA13700.012.8284672
Produced by FastQC (version 0.11.5)