FastQCFastQC Report
Thu 9 Feb 2017
SRR2727188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3442585
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC418641.216062929455627No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG384311.1163413539534972No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA296440.861097111618159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230200.6686835619164088No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG222690.6468685595272157No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG211790.615206305726656No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT190100.5522013254574687No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC183530.5331168293593331No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG183010.5316063365174716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133590.3880514206620897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131810.38288088747264043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130490.3790465594894534No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA129980.37756511458685843No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC112190.32588883063163293No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT111130.32280974906937665No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC110950.3222868861625784No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT107720.31290440177947676No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG106920.31058056663815126No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT98230.28533790741550313No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC95620.2777563952669288No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT86730.2519327772589493No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC85070.24711081934069892No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC83800.2434217310538447No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC74720.21704620219980042No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71760.20844801217689612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC71670.20818658072349702No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT70720.20542702649317301No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT70520.20484606770784167No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA67820.19700312410586815No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT67510.19610263798860447No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT66800.19404023430067813No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG61930.17989388787785923No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61420.17841244297526423No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT59310.17228332779001826No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG59250.17210904015441886No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT57630.16740327399323474No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG56930.16536991824457492No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC51080.14837687377363232No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT49850.1448039772438444No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT47030.13661245837067204No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG46950.1363800748565395No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA45280.13152906899902253No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT43510.1263875837488399No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG43360.12595186465984137No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG41720.12118800262012414No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT41640.12095561910599158No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG40760.11839940045053354No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT40150.11662747615527286No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.11508793537414472No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT39280.1141003054390814No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG37770.10971406660982953No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37010.1075064232255703No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA36890.1071578479543715No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT36410.1057635468695762No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA34700.10079634925499298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG652.6854304E-622.769235
AAAAAGT23050.020.0650751
AAAAGTA23600.019.6758482
TCGTCGA609.24192E-418.513
TATCACG1603.45608E-1117.343752
AAAGTAC29500.015.9288143
AAGTACT30350.015.909394
GTACCGT700.002593902215.8571436
CAAGACG7950.015.8238984
AGTACTC30150.015.5854075
AAGACGG7800.015.4166675
TCGTTAC1701.4897523E-915.23529423
CGAACGT4450.014.9662914
TAATACT2850.014.9298244
TCGAACG4700.014.5638283
AACGTCT5100.014.14705856
ATTCGAA4750.014.0210521
ATACCGA800.006301988413.8756
TTAGACT2405.456968E-1213.8749994
TATACTG2405.456968E-1213.8749995
Produced by FastQC (version 0.11.5)