##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727188.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3442585
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.968239273685327	31.0	31.0	33.0	30.0	34.0
2	31.062750520321213	31.0	31.0	34.0	28.0	34.0
3	31.126931070692518	31.0	31.0	34.0	28.0	34.0
4	34.943023629046195	35.0	35.0	37.0	32.0	37.0
5	34.786836635841965	35.0	35.0	37.0	32.0	37.0
6	34.88812157143542	35.0	35.0	37.0	32.0	37.0
7	34.69249561013018	35.0	35.0	37.0	31.0	37.0
8	34.671966560012315	35.0	35.0	37.0	31.0	37.0
9	36.261954025826526	38.0	35.0	39.0	32.0	39.0
10	36.03612663158644	38.0	35.0	39.0	30.0	39.0
11	36.16627766634666	38.0	35.0	39.0	32.0	39.0
12	36.053307325745045	38.0	35.0	39.0	30.0	39.0
13	36.14737878658043	38.0	35.0	39.0	31.0	39.0
14	37.06072675039251	39.0	36.0	40.0	31.0	41.0
15	36.96014680828505	39.0	36.0	40.0	31.0	41.0
16	36.86029974568529	38.0	36.0	40.0	31.0	41.0
17	36.849176418301944	38.0	36.0	40.0	31.0	41.0
18	36.83104353269418	38.0	36.0	40.0	31.0	41.0
19	36.828011218314145	39.0	36.0	40.0	31.0	41.0
20	36.77722118698594	39.0	36.0	40.0	31.0	41.0
21	36.62041750603108	38.0	35.0	40.0	30.0	41.0
22	36.62690187751355	38.0	35.0	40.0	30.0	41.0
23	36.38096691875437	38.0	35.0	40.0	30.0	41.0
24	36.27146896881268	38.0	35.0	40.0	30.0	41.0
25	36.1825936033533	38.0	34.0	40.0	29.0	41.0
26	35.80313340120868	38.0	34.0	40.0	27.0	41.0
27	35.92876806237174	38.0	34.0	40.0	29.0	41.0
28	35.773923955399795	38.0	34.0	40.0	27.0	41.0
29	35.66152673063991	38.0	34.0	40.0	27.0	41.0
30	35.7238583796769	38.0	34.0	40.0	27.0	41.0
31	35.61957453483356	38.0	34.0	40.0	27.0	41.0
32	35.32022883966554	38.0	34.0	40.0	26.0	41.0
33	35.25528578088849	38.0	34.0	40.0	26.0	41.0
34	35.225030609266	38.0	34.0	40.0	26.0	41.0
35	34.926752135386636	38.0	33.0	40.0	25.0	41.0
36	34.783563804524796	38.0	33.0	40.0	24.0	41.0
37	34.52336514566815	38.0	33.0	40.0	24.0	41.0
38	34.35402669796098	38.0	33.0	40.0	23.0	41.0
39	34.263248402000244	38.0	33.0	40.0	23.0	41.0
40	34.071208989756244	37.0	33.0	40.0	21.0	41.0
41	33.87605970513437	37.0	33.0	40.0	21.0	41.0
42	33.66691018522418	37.0	32.0	40.0	19.0	41.0
43	33.18329075389569	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	6.0
13	7.0
14	17.0
15	50.0
16	173.0
17	340.0
18	733.0
19	1510.0
20	2829.0
21	5319.0
22	8783.0
23	13999.0
24	21560.0
25	31673.0
26	44827.0
27	61900.0
28	81835.0
29	104893.0
30	128868.0
31	153603.0
32	181121.0
33	211660.0
34	251020.0
35	296596.0
36	358376.0
37	452450.0
38	583266.0
39	445167.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.86586968803965	20.05161818807669	12.484542865317778	24.597969258565875
2	16.34460732269501	26.771161786854936	31.579699557164165	25.30453133328589
3	22.837257467862084	27.494891193681493	28.82903399625572	20.838817342200702
4	16.614114103210234	18.46281210195246	31.132332244519745	33.79074155031756
5	13.860282316921731	38.416219207368876	32.902542711363694	14.820955764345689
6	33.08284327039129	31.646422673659476	17.281403364041847	17.98933069190739
7	24.639856386988264	31.668179580170136	23.76554246300382	19.926421569837782
8	27.61361012146396	31.71029909210666	23.727141087293415	16.94894969913597
9	27.910305773132688	14.183440641262306	20.959976296881557	36.946277288723444
10	18.60912657203816	27.406759745946722	32.72892317836742	21.255190503647693
11	32.30740853167024	23.080301575705462	20.869259582552065	23.74303031007223
12	21.303410082830197	28.78970308648879	29.450921328013685	20.45596550266733
13	30.679910590442937	19.11037781202207	25.88996350126431	24.31974809627068
14	22.776750610369824	22.732801078259506	26.90588612917328	27.58456218219739
15	28.362640283391695	24.32523815679206	24.362390471114004	22.94973108870224
16	21.878414040611922	25.59960030035569	30.22423556716828	22.297750091864106
17	24.659667081568067	27.830830611299355	27.382795196051806	20.126707111080773
18	24.587541048369175	21.998614413296984	30.870494119970893	22.543350418362945
19	23.79168560834373	29.025485209515523	28.36075216733937	18.822077014801376
20	23.080737294794464	24.383450227082264	30.02130666345203	22.514505814671242
21	21.746245916949036	27.854098010651878	28.953794895405633	21.44586117699345
22	23.138077926906668	27.647567162466576	24.911599858827017	24.30275505179974
23	20.019258783733733	26.45944835058539	28.148208395725888	25.373084469954993
24	21.142774978686074	30.938379154036866	26.791379152584465	21.127466714692595
25	23.369531906982687	26.32559544644504	24.462170142494667	25.842702504077604
26	22.508841466514262	26.69215139204987	27.90989910198296	22.88910803945291
27	24.0865221918994	25.940826442920073	25.580690091893153	24.391961273287368
28	21.84846561522809	27.137049629856634	28.906504850279656	22.107979904635616
29	22.906769186526983	24.942506866206642	28.963264523606536	23.18745942365984
30	21.389362935119976	28.647484375839667	29.826104511580688	20.137048177459672
31	25.48459950879935	25.732175095168312	24.88496289851957	23.89826249751277
32	18.780335126075318	27.99062332520475	28.425267640450418	24.803773908269513
33	21.68414142279711	26.84840025736474	29.080414862668608	22.38704345716954
34	20.66316445345576	26.512606079443206	28.712464615978984	24.11176485112205
35	20.54441647773403	28.91995404616008	29.900902955192098	20.63472652091379
36	23.26086356618646	26.794719665600127	26.972667341547123	22.9717494266663
37	21.210427629237913	27.58523028480052	28.463029961496954	22.74131212446461
38	22.59746672921656	26.61685913347092	28.83301356393524	21.952660573377273
39	22.864678722529728	23.569614112650815	28.09153586621681	25.47417129860265
40	22.037015789007388	26.35037333863942	28.884079841165867	22.72853103118732
41	20.62092874976217	25.8637332121066	28.943773356358665	24.571564681772564
42	22.53248648907725	27.3259774268464	28.40089641940577	21.74063966467059
43	21.79795124884353	24.03159253874632	29.431982071611884	24.738474140798267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	946.0
1	1170.0
2	1394.0
3	5018.5
4	8643.0
5	8643.0
6	13068.5
7	17494.0
8	17935.5
9	18377.0
10	21697.5
11	25018.0
12	25018.0
13	36331.5
14	47645.0
15	60101.0
16	72557.0
17	64156.0
18	55755.0
19	55755.0
20	55273.5
21	54792.0
22	45480.5
23	36169.0
24	35926.0
25	35683.0
26	35683.0
27	37342.0
28	39001.0
29	40739.5
30	42478.0
31	46140.5
32	49803.0
33	49803.0
34	58915.5
35	68028.0
36	81291.5
37	94555.0
38	108321.5
39	122088.0
40	122088.0
41	133023.5
42	143959.0
43	162618.0
44	181277.0
45	219373.5
46	257470.0
47	257470.0
48	413663.5
49	569857.0
50	561467.5
51	553078.0
52	388681.5
53	224285.0
54	224285.0
55	194351.0
56	164417.0
57	157569.0
58	150721.0
59	139821.5
60	128922.0
61	128922.0
62	106952.0
63	84982.0
64	73871.5
65	62761.0
66	53320.0
67	43879.0
68	43879.0
69	37194.5
70	30510.0
71	26850.0
72	23190.0
73	17589.5
74	11989.0
75	11989.0
76	9328.5
77	6668.0
78	5873.5
79	5079.0
80	4292.5
81	3506.0
82	3506.0
83	2943.0
84	2380.0
85	2116.0
86	1852.0
87	1313.5
88	775.0
89	775.0
90	543.0
91	311.0
92	225.5
93	140.0
94	137.5
95	135.0
96	135.0
97	71.0
98	7.0
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3442585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.75926024807698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8931979802693	31.29985835332358
2	8.488230090294232	6.410185780499557
3	2.999694417924699	3.3979872657336747
4	1.4703666327310623	2.2207982538152313
5	0.8885630438823849	1.6775741610389208
6	0.5789426198556387	1.3116267031099533
7	0.42300062967011115	1.118053360256991
8	0.3292226131149498	0.9944961862527482
9	0.23208613385514726	0.7887060655385879
>10	1.4071672400176887	9.69689194747154
>50	0.12812043090774702	3.3907783975205703
>100	0.12776659540627489	10.255035708625039
>500	0.019167872459063418	4.975010734749899
>1k	0.011500723475436875	8.241730023971739
>5k	0.001564724282372364	4.190508899492235
>10k+	0.0014082518541351275	10.03075815859973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	41864	1.216062929455627	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	38431	1.1163413539534972	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	29644	0.861097111618159	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23020	0.6686835619164088	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	22269	0.6468685595272157	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	21179	0.615206305726656	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	19010	0.5522013254574687	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	18353	0.5331168293593331	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	18301	0.5316063365174716	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13359	0.3880514206620897	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13181	0.38288088747264043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13049	0.3790465594894534	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	12998	0.37756511458685843	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	11219	0.32588883063163293	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	11113	0.32280974906937665	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	11095	0.3222868861625784	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	10772	0.31290440177947676	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	10692	0.31058056663815126	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	9823	0.28533790741550313	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	9562	0.2777563952669288	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	8673	0.2519327772589493	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	8507	0.24711081934069892	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	8380	0.2434217310538447	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	7472	0.21704620219980042	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	7176	0.20844801217689612	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	7167	0.20818658072349702	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	7072	0.20542702649317301	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	7052	0.20484606770784167	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	6782	0.19700312410586815	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	6751	0.19610263798860447	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	6680	0.19404023430067813	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6193	0.17989388787785923	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6142	0.17841244297526423	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	5931	0.17228332779001826	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	5925	0.17210904015441886	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	5763	0.16740327399323474	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	5693	0.16536991824457492	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	5108	0.14837687377363232	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	4985	0.1448039772438444	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	4703	0.13661245837067204	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	4695	0.1363800748565395	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	4528	0.13152906899902253	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	4351	0.1263875837488399	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	4336	0.12595186465984137	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4172	0.12118800262012414	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	4164	0.12095561910599158	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	4076	0.11839940045053354	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	4015	0.11662747615527286	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3962	0.11508793537414472	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	3928	0.1141003054390814	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	3777	0.10971406660982953	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3701	0.1075064232255703	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	3689	0.1071578479543715	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	3641	0.1057635468695762	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	3470	0.10079634925499298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	2.904793926656858E-5
9	0.0	0.0	0.0	0.0	8.714381779970575E-5
10	0.0	0.0	0.0	2.0333557486598006E-4	8.714381779970575E-5
11	0.0	0.0	0.0	5.228629067982345E-4	8.714381779970575E-5
12	0.0	0.0	0.0	9.295340565301946E-4	8.714381779970575E-5
13	0.0	0.0	0.0	0.0012781093277290177	8.714381779970575E-5
14	2.904793926656858E-5	0.0	0.0	0.002265739262792349	1.1619175706627432E-4
15	2.904793926656858E-5	0.0	0.0	0.0037181362261207783	1.1619175706627432E-4
16	2.904793926656858E-5	0.0	0.0	0.0075815121485744	1.4523969633284292E-4
17	2.904793926656858E-5	0.0	0.0	0.012316326249025079	1.742876355994115E-4
18	2.904793926656858E-5	0.0	0.0	0.015337311932748211	2.0333557486598006E-4
19	2.904793926656858E-5	0.0	0.0	0.023267399352521433	2.0333557486598006E-4
20	2.904793926656858E-5	0.0	0.0	0.029541754234100246	2.0333557486598006E-4
21	2.904793926656858E-5	0.0	0.0	0.04577955228411208	2.0333557486598006E-4
22	2.904793926656858E-5	0.0	0.0	0.0673912190984391	2.0333557486598006E-4
23	2.904793926656858E-5	0.0	0.0	0.09388293970954965	2.6143145339911725E-4
24	2.904793926656858E-5	0.0	0.0	0.13826819090886644	2.6143145339911725E-4
25	2.904793926656858E-5	0.0	0.0	0.16551515794090776	2.6143145339911725E-4
26	2.904793926656858E-5	0.0	0.0	0.2157680928720714	2.6143145339911725E-4
27	2.904793926656858E-5	0.0	0.0	0.30108189049798334	2.6143145339911725E-4
28	2.904793926656858E-5	0.0	0.0	0.4504173462674124	2.6143145339911725E-4
29	2.904793926656858E-5	0.0	0.0	0.5742487113607943	2.6143145339911725E-4
30	2.904793926656858E-5	0.0	0.0	0.7724137530373252	2.6143145339911725E-4
31	2.904793926656858E-5	0.0	0.0	1.128948159595188	2.6143145339911725E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	65	2.6854304E-6	22.76923	5
AAAAAGT	2305	0.0	20.065075	1
AAAAGTA	2360	0.0	19.675848	2
TCGTCGA	60	9.24192E-4	18.5	13
TATCACG	160	3.45608E-11	17.34375	2
AAAGTAC	2950	0.0	15.928814	3
AAGTACT	3035	0.0	15.90939	4
GTACCGT	70	0.0025939022	15.857143	6
CAAGACG	795	0.0	15.823898	4
AGTACTC	3015	0.0	15.585407	5
AAGACGG	780	0.0	15.416667	5
TCGTTAC	170	1.4897523E-9	15.235294	23
CGAACGT	445	0.0	14.966291	4
TAATACT	285	0.0	14.929824	4
TCGAACG	470	0.0	14.563828	3
AACGTCT	510	0.0	14.1470585	6
ATTCGAA	475	0.0	14.021052	1
ATACCGA	80	0.0063019884	13.875	6
TTAGACT	240	5.456968E-12	13.874999	4
TATACTG	240	5.456968E-12	13.874999	5
>>END_MODULE