FastQCFastQC Report
Thu 9 Feb 2017
SRR2727187.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727187.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8446332
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC927561.098180843471462No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG888031.051379462706415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT643930.7623782726040131No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA602140.7129011741428114No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG508070.6015273849050689No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG419010.496085164542431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT410620.48615185858192644No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC389140.46072070100962165No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT375650.44474927104451967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT373930.44271288412532206No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG356330.42187543658004445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346580.4103319642183139No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT288760.34187621324854384No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC281200.3329255823711405No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG262940.31130673054291497No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT258000.30545803787963816No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT256070.30317302232495713No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC254340.30112479594692704No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA245930.2911678110687574No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT202540.23979639919434853No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC200870.23781920956931366No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT188240.22286597306381042No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC185860.22004818186166492No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC178100.2108607618076107No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT177530.2101859126541557No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC172910.2047160826735203No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT164660.19494852913667138No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA162940.1929121422174738No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162360.19222545360518625No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT150010.17760372194699428No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG149910.17748532735866884No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT144980.17164847415422457No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG143830.17028693638848202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC143550.16995543154117077No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT141410.16742178735100632No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG128810.15250406922200074No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT128420.1520423303275315No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC119250.14118554657808857No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115800.13710093328086084No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG111200.13165478221789056No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109210.12929872991021427No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG107610.1274044164970072No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT107210.12693083814370545No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT103970.12309485348196117No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT102620.12149652653956772No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG101990.12075064063311744No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA98250.11632268302974594No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT95060.11254589566216436No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC94090.11139746815540758No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC92950.11004776984849754No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT91980.10889934234174076No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG90740.10743124944650528No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT89780.10629466139858107No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG87600.10371365937308645No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA87200.10324008101978469No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT86500.10241131890150659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA48600.016.5586432
TATCACG3950.015.924052
AAAAAGT51200.015.6816411
TGGGCTG74550.014.4178436
ACCGTCG19700.014.1802038
GGGCTGT79400.013.90994937
AGTACTC58800.013.8755
AAAGTAC59300.013.8204053
TACCGTC20250.013.7950627
GGTTATC15900.013.7295592
AGGTTAT15450.013.6504851
AAGTACT60800.013.6315794
ATACCGT20900.013.6315786
CAAGACG22050.013.50793654
AAGACGG21700.013.4700465
CGAGCCG21650.013.41570415
CACTCTA22550.013.1263869
ACGAACG11700.013.12393215
CCGTCGT20600.013.0218459
CTTATAC104400.012.98898537
Produced by FastQC (version 0.11.5)